This study focuses on evaluating microbial activity in Main Dolomite reservoir rocks (Zechstein, Wuchiapingian– Permian) at 100°C. Core samples from two wells, one potentially microbiologically active (C-1 well, 100°C) and the other considered inactive due to high temperature (L-1 well, 155.5°C), were analysed. The core from L-1, treated similarly to C-1, was used to control for contamination. Microbial experiments and molecular analyses were performed on both core samples to ensure accurate results unaffected by laboratory processing contamination. Microbial incubation tests were successfully employed to demonstrate microbial hydrocarbon degradation and methane formation from 13C-labelled acetate. A new method for staining microorganisms from rock samples was introduced to estimate biomass. The results indicated that microorganisms from the C-1 well exhibited both hydrocarbon biodegradation and acetoclastic methanogenesis during a 3-month incubation at 100°C. Fluorescence-stained and countable microorganisms were only observed in the C-1 samples, while no biodegradation or methanogenesis occurred in reference samples from L-1 well. The study provides valuable insights into microbial activities in extreme conditions, emphasizing the importance of proper controls and techniques to ensure accurate interpretation of results.

Andrzej Borkowski

Paweł Zdanowski

Paweł Działak

Tomasz Kowalski

Tomasz Segit

Acta Geologica Polonica

ACTA GEOLOGICA POLONICA 发表关于基础地质学各个方面的原创和评论论文，特别关注沉积学、地层学、古生物学、区域地质学、构造地质学和区域岩相学。所有论文均以英文发表。

Acta Geologica Polonica publishes original and review papers on various aspects of fundamental geology, with a particular focus on sedimentology, stratigraphy, paleontology, regional geology, structural geology, and regional petrology. All papers are published in English.

《Acta Geologica Polonica》发表关于基础地质学各个方面的原创和评论论文，特别关注沉积学、地层学、古生物学、区域地质学、构造地质学和区域岩相学。所有论文均以英文发表。

Category	Quartile
地球科学	4
地球科学, 地质学	4

Deep biosphere in the Main Dolomite (Upper Permian) hydrocarbon reservoir

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