Our previous research shown that tumor necrosis factor-alpha-induced protein 8 (TNFAIP8) is elevated in the plasma extracellular vesicles and vitreous humor in diabetic retinopathy (DR). TNFAIP8 also significantly increases the viability of human retinal microvascular endothelial cells (HRMECs) and promotes cell migration and tube formation in vitro. To comprehensively explore its role in DR, we investigated the effect of TNFAIP8 on DR development using an animal model in this study. A TNFAIP8-overexpressing adeno-associated virus (AAV) vector and streptozotocin-induced mouse model was used. The AAV-TNFAIP8 vector was injected into the mice intravitreally, and the effect was evaluated. The evaluation included analysis of retinal structure and function using electroretinography, optical coherence tomography, and histological assessment. The influence of TNFAIP8 on the avascular area, retinal leukostasis, and the expression levels of inflammatory factors was also determined. TNFAIP8 significantly decreased a/b-wave amplitude and retinal thickness in diabetic mice. Histological assessment showed that TNFAIP8 aggravated pathological abnormalities with distorted organization of the retina. TNFAIP8 also significantly increased the avascular area, leukostasis, and the expression of inflammatory factors, such as TNFα, IL1β, ICAM1, and GFAP, in the retina. The results of this study support the role of TNFAIP8 in DR pathogenesis. A mechanistic understanding of TNFAIP8 may offer novel therapeutic strategies.

Fuhua Yang

Hui Zhang

Xinyue Yu

Qingqin Tao

Chuan Zhao

Jinying An

Xiaomin Zhang

Xiaorong Li

Experimental eye research

EXPERIMENTAL EYE RESEARCH 的主要目标是发表关于眼睛和眼组织实验生物学各个方面的原创研究论文，旨在确定正常功能和/或疾病的机制。包括细胞生物学、发育生物学、遗传学、分子生物学、生理学、生物化学、生物物理学、免疫学或微生物学等学科的眼组织研究是最受欢迎的。纯临床或眼科外科领域的手稿不适合提交给 EXPERIMENTAL EYE RESEARCH，如果收到，将不经审查退回。

The primary aim of Experimental Eye Research is to publish original research papers on various aspects of experimental biology of the eye and its tissues, with the goal of elucidating the mechanisms of normal function and/or disease. The journal particularly welcomes studies on ocular tissues that involve disciplines such as cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology, or microbiology. Please note that manuscripts focused solely on clinical or ophthalmic surgical fields are not suitable for submission to Experimental Eye Research and will be returned without review if received.

《Experimental Eye Research》的主要目标是发表关于眼睛及其组织实验生物学各个方面的原创研究论文，旨在揭示正常功能和疾病机制。该期刊特别欢迎涉及细胞生物学、发育生物学、遗传学、分子生物学、生理学、生物化学、生物物理学、免疫学和微生物学等学科的眼组织研究。请注意，纯临床或眼科外科领域的手稿不适合提交给《Experimental Eye Research》，如果收到此类稿件，将不经审查直接退回。

Experimental Eye Research

Exp Eye Res

TNFAIP8 overexpression aggravates retinal pathophysiological features of diabetic retinopathy.

The results of this study support the role of TNFAIP8 in DR pathogenesis and suggest that a mechanistic understanding of TN FAIP8 may offer novel therapeutic strategies.

Category	Quartile
医学	2
医学, 眼科学	2

TNFAIP8 overexpression aggravates retinal pathophysiological features of diabetic retinopathy.

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