β-Galactosidase (β-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyzes the β-galactosides into monosaccharides. β-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve β-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of β-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of β-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The β-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in color and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation β-Gal-related diseases has boosted the research in this fertile field.

Beatriz Lozano-Torres

Juan F Blandez

Félix Sancenón

Ramón Martínez-Máñez

Analytical and bioanalytical chemistry

分析和生物分析化学的使命是快速发表关于分析和生物分析测量科学的基础和应用主题的优秀和高影响力的研究文章。它的范围很广，从新颖的测量平台及其表征到有效解决重要科学问题的多学科方法。编辑鼓励提交概念、仪器、方法和/或应用方面的创新分析研究，包括：质谱、光谱学和电分析；高级分离； “组学”和成像、生物分析和取样中的分析策略；小型化设备、医疗诊断、传感器；纳米和生物材料的分析表征；化学计量学和高级数据分析。

The mission of "Analytical and Bioanalytical Chemistry" is to rapidly publish outstanding and high-impact research articles on fundamental and applied topics in analytical and bioanalytical measurement science. The scope is broad, ranging from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address significant scientific issues. The editors encourage submissions of innovative analytical research in concepts, instruments, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separation techniques; analytical strategies in omics and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, and sensors; analytical characterization of nanomaterials and biomaterials; chemometrics and advanced data analysis.

《Analytical and Bioanalytical Chemistry》的使命是快速发表关于分析和生物分析测量科学的基础和应用主题的优秀、高影响力的研究文章。期刊的范围广泛，涵盖从新颖的测量平台及其表征，到有效解决重要科学问题的多学科方法。编辑鼓励提交在概念、仪器、方法和/或应用方面的创新分析研究，包括：质谱、光谱学和电分析；高级分离技术；“组学”及成像、生物分析和取样中的分析策略；小型化设备、医疗诊断、传感器；纳米和生物材料的分析表征；化学计量学和高级数据分析。

Analytical and Bioanalytical Chemistry

Anal Bioanal Chem

Chromo-fluorogenic probes for β-galactosidase detection.

In this review, the progress made to date in the design of molecular-based probes for the detection of β-Gal both in vitro and in vivo are assessed.

Category	Quartile
化学	2
化学, 生化研究方法	3
化学, 分析化学	3

Chromo-fluorogenic probes for β-galactosidase detection.

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info

Category	Quartile
CHEMISTRY, ANALYTICAL	1