ACTA VIROLOGICA 是一份以分子和细胞病毒学为主的国际期刊。 ACTA VIROLOGICA 旨在发表论文，报告主要在细胞和分子水平上对人类、动物和植物病毒的基础和应用研究的原始结果。按照传统，立克次体也包括在内。感兴趣的领域是病毒结构和形态、病毒-细胞相互作用的分子生物学、病毒的分子遗传学、病毒疾病的发病机制、病毒免疫学、疫苗、抗病毒药物和病毒诊断。

Acta Virologica is an international journal focused on molecular and cellular virology. The journal aims to publish original research papers reporting fundamental and applied studies on human, animal, and plant viruses, particularly at the cellular and molecular levels. Traditionally, rickettsiae are also included in the scope of the journal. Areas of interest include virus structure and morphology, molecular biology of virus-cell interactions, molecular genetics of viruses, pathogenesis of viral diseases, virology immunology, vaccines, antiviral drugs, and viral diagnostics.

《Acta Virologica》是一本国际期刊，专注于分子和细胞病毒学。该期刊旨在发表关于人类、动物和植物病毒的基础和应用研究的原创论文，特别是在细胞和分子层面上的研究成果。根据传统，立克次体也被纳入研究范围。感兴趣的研究领域包括病毒的结构与形态、病毒与细胞相互作用的分子生物学、病毒的分子遗传学、病毒疾病的发病机制、病毒免疫学、疫苗、抗病毒药物以及病毒诊断等。

Acta Virologica

S-L Chia

W-S Tan

K Yusoff

N Shafee

Acta virologica

Acta Virol

Plaque formation by a velogenic Newcastle disease virus in human colorectal cancer cell lines.

人结直肠癌细胞系中强毒性新城疫病毒引起的斑块形成。

Duck enteritis virus (DEV) causes a contagious, acute and highly lethal disease in all ages of birds from the order Anseriformes. DEV leads to heavy economic losses to the commercial duck industry due to its high mortality rate and decrease in egg production. With development of molecular biology, more information about DEV genes is reported, nonetheless little information is known about DEV UL29 gene and its product major DNA-binding protein or infected-cell protein 8 (ICP8). The sequence characteristics of DEV UL29 gene was initially showed in our article. Phylogenetic tree analysis provided useful proof that DEV belongs to the subfamily Alphaherpesvirinae. The predicted characteristics of ICP8 amino acid sequence showed that ICP8 possesses good immunogenicity and is a hydrophobic protein. These findings correlate with the experimental results that ICP8 protein forms inclusion bodies in the prokaryotic expression system. By immunofluorescence we have identified ICP8 as nuclear protein. All the fundamental data in this article contribute to understanding the functions of DEV UL29 gene and its product ICP8.

A Cheng

S Zhang

X Zhang

M Wang

D Zhu

R Jia

X Chen

Prokaryotic expression and characteristics of duck enteritis virus UL29 gene

鸭瘟病毒UL29基因的原核表达及特性研究

Background India witnessed three COVID-19 pandemic waves, each with various degrees of severity and clinical signs. The coronavirus strain and immunization status have a significant impact on the severity of COVID-19 infections. The current study intends to evaluate and compare the symptoms, severity, and breakthrough infections in vaccinated and unvaccinated individuals over the three waves of the pandemic. Methods This was a retrospective survey study. A Google based questionnaire was used to collect data on demographics, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection status, re-infections, associated symptoms, severity, and vaccination status over three waves, post-COVID-19 sequelae, and willingness to receive a COVID-19 booster dose in the Indian population. The replies of 3,404 Indian participants were analysed using STATA 11. Results Each wave showed a decrease in the number of symptomatic COVID-19 infections. However, fever and loss of smell/taste were identified as the most common symptoms in each wave. Clinical symptoms such as fever, weariness, and shortness of breath were shown to be considerably higher in vaccinated than unvaccinated individuals. The number of SARS-CoV-2 breakthrough infections increased between the second and third waves. Approximately 36.5% of people with protracted COVID-19 had previously received immunization after recovering from a natural COVID-19 illness. Overall, 34.8% of individuals were hesitant to take the COVID-19 booster dose. Conclusion Increased symptoms in vaccinated individuals during the second wave, emphasizing the potential role of antibody-dependent augmentation. A considerable fraction (36.5%) of those with protracted COVID-19 infections had previously received vaccination after contracting the virus naturally. The fact that vaccine received after COVID-19 infection has been shown to be a risk factor for long-term COVID-19 emphasizes the need for vigilance in this specific subgroup.

Priya Bhardwaj

Sunita Jetly

Sunita Yadav

Rahul Kaushik

Kumar Naidu

Daman Saluja

Jyoti Taneja

Comparative analyses of symptoms, severity, and breakthrough infections in vaccinated and unvaccinated individuals during three waves of COVID-19 in India

印度三波COVID-19疫情期间接种与未接种疫苗个体症状、严重程度及突破性感染的比较分析

Enterovirus 71 (EV71) is a significant causative agent of hand, foot and mouth disease (HFMD). However, the precise mechanism by which EV71 infection leads to alterations in the immune response remain elusive. To address this knowledge gap, we conducted a study where we introduced the Inner Mongolia EV71 C33λ strain into Vero cells, derived from African green monkey kidney cells. Subsequently, we performed RNA sequencing (RNA-Seq) to investigate the changes in the transcriptome of these infected Vero cells. Our primary objective was to establish a foundational understanding that could inform future research on EV71-associated immune factors. In our study, we identified a total of 942 differentially expressed genes (DEGs) in Vero cells infected with Enterovirus 71 (EV71), with 568 gene exhibiting increased expression and 374 gene showing decreased expression. To elucidate the functional implications of these DEGs, we conducted a comprehensive functional enrichment analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. This analysis revealed three genes that were significantly upregulated, which we subsequently validated using reverse transcription polymerase chain reaction technology (RT-qPCR). The RT-qPCR results were in strong agreement with our RNA-Seq data, confirming the reliability of our findings. This study represents the pioneering RNA-Seq analysis that delves into the cellular response of Vero cells to EV71 infection. Our results not only provide a foundational understanding of the molecular changes induced by EV71 but also offer crucial insights into the mechanisms by which EV71 modulates the host immune system. These insights are pivotal for future research endeavors aimed at developing effective therapeutic strategies against EV71 and related pathogens, as well as for understanding the broader implications of viral infections on host immunity.

Xiong Lai

Yaochun Fan

Hongxia Li

Xiaoling Tian

Xuanzhi Yue

Sheng Gao

Xia Lei

Yuhan Qin

Xiangnan Zhang

Jun Mei

Yu Shi

Huan Li

Duoduo Wu

Wenyu Zhang

Xinrui Jia

Ruichun Fan

Bin Li

Wanjin Xing

A RNA-seq-based study on differentially expressed genes related to the Inner Mongolia EV71 C33λ strain that invades Vero cells

基于RNA-seq的内蒙EV71 C33λ株侵染Vero细胞差异表达基因研究

Background Currently, there are approximately 240 million individuals worldwide suffering from chronic hepatitis B infection, yet a definitive cure remains elusive. In the context of pharmaceutical interventions for this patient population, the potential issue of Hepatitis B virus (HBV) reactivation must be taken into consideration. Nutlin-3 is a small molecule of imidazoline analog, which inhibits tumor growth by suppressing the interaction between human double minute 2 (HDM2) and p53. However, whether it affects HBV replication is not clear. Methods HepG2.2.15 cells were used to assess the impact of Nutlin-3 on HBV replication. The expression of Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg) was detected by ELISA. The expression of pregenomic RNA (pgRNA) and core-DNA was detected by RT-qPCR. The activation of relevant signaling pathways was assessed through Western blotting and confocal microscopy detection of the expression of associated proteins. Results We found that Nutlin-3 significantly enhances the expression of HBsAg and HBeAg, and it also promotes the expression of pgRNA and Core-DNA. Nutlin-3 promotes HBV replication independent of the HDM2. Instead, it activates the autophagy pathway through p53-mediated mTOR inhibition to promote HBV replication. Conclusion When using Nutlin-3 for cancer therapy, it’s important to be aware of the potential side effect of promoting HBV replication in individuals with chronic hepatitis B infection.

Shaowei Han

Yuanyuan Li

Junnan Wang

Yong Zhang

Nutlin-3 promotes HBV replication by activating mTOR-mediated autophagy

Nutlin-3通过激活mTOR介导的自噬促进HBV复制

The global pandemic of COVID-19 caused by SARS-CoV-2 has had a devastating impact. Although many survived the acute effects of the pandemic, a significant number of survivors, including those with only mild symptoms, are now experiencing a prolonged and debilitating post-viral syndrome known as LC/PASC (long COVID/post-acute sequelae of SARS-CoV-2). Typical symptoms of LC/PASC include fatigue, breathlessness, chest pain, impaired cognition, difficulty sleeping, fever and gastrointestinal symptoms. Anxiety and depression can also last for weeks to months and range from mild to disabling. The association between neuropsychiatric symptoms and SARS-CoV-2 infection raises questions about the possible routes of SARS-CoV-2 entry to the central nervous system (CNS) and long-term effects of the virus on the CNS, their molecular basis, and the potential risk of neuronal damage associated with the subsequent development of neurodegenerative diseases.

Weronika Daria Krahel

Michalina Bartak

Joanna Cymerys

Acute and long-term SARS-CoV-2 infection and neurodegeneration processes—circulus vitiosus

急性与长期SARS-CoV-2感染及神经退行性过程——恶性循环

Enterovirus (EV) infections occur frequently in humans. In some geographical areas they are more common. These viruses cause diseases with varying degrees of severity, from a simple respiratory tract infection to severe diseases. Since EVs include more than 70 serotypes currently circulating in the population, a methodology that detects most of them is needed. ELISA is a rapid, sensitive, and economical diagnostic method for the identification of EV serotypes and can also be used as a retrospective diagnostic tool or in the investigation of outbreaks of infection. Commercial EV-ELISAs often appear and gradually disappear from the market supply. We have used the KTL-510 peptide, a synthetic viral protein of poliovirus VP1, as an antigen in a peptide-based ELISA for the detection of a broader spectrum of anti-EV antibodies. We aimed to design, optimize, and standardize this in-house ELISA with the peptide, and implement the method for routine detection of anti-EV IgG in human sera. For determining the cut-off value, we used 100 patients’ sera which were previously tested negative for IgG antibodies against EVs using a commercial ELISA kit available. We monitored patients’ sera samples sent for serological testing of anti-coxsackievirus antibodies to the National Reference Center for the Identification of Enteric Viruses between 2018–2022. These serum samples were examined using a standard virus neutralization test as well as the newly developed ELISA method.

Michaela Pellerova

Katarina Albertova

Vanesa Simkova

Maria Borsanyiova

Brigita Benkoova

Renata Kissova

Katarina Pastuchova

Sisko Tauriainen

Jochem M. D. Galama

Shubhada Bopegamage

Detection of anti-enterovirus IgG in human sera by ELISA method using the KTL-510 peptide

通过使用KTL-510肽的ELISA方法检测人血清中的抗肠道病毒IgG

Abbreviation	Acta Virol.
Journal Impact	1.10
Quartiles(Global)	VIROLOGY(Q4)

ISSN	0001-723X, 1336-2305
h-index	36

Publication Information	Publisher: Frontiers Media SA，Publishing cycle: Bimonthly，Journal Type: journal，Open Access Journals: Yes
Basic data	Year of publication: 1957，Proportion of original research papers: 97.44%，Self Citation Rate:18.20%， Gold OA Rate: 34.43%
Journal Fees	$Details
Average review cycle	网友分享经验：>12周，或约稿
Average recruitment ratio	网友分享经验：容易

Acta Virologica

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Plaque formation by a velogenic Newcastle disease virus in human colorectal cancer cell lines.

Prokaryotic expression and characteristics of duck enteritis virus UL29 gene

Comparative analyses of symptoms, severity, and breakthrough infections in vaccinated and unvaccinated individuals during three waves of COVID-19 in India

A RNA-seq-based study on differentially expressed genes related to the Inner Mongolia EV71 C33λ strain that invades Vero cells

Nutlin-3 promotes HBV replication by activating mTOR-mediated autophagy

Comparative analyses of symptoms, severity, and breakthrough infections in vaccinated and unvaccinated individuals during three waves of COVID-19 in India

Nutlin-3 promotes HBV replication by activating mTOR-mediated autophagy

A RNA-seq-based study on differentially expressed genes related to the Inner Mongolia EV71 C33λ strain that invades Vero cells

Acute and long-term SARS-CoV-2 infection and neurodegeneration processes—circulus vitiosus

Detection of anti-enterovirus IgG in human sera by ELISA method using the KTL-510 peptide