Archives of Andrology

cAMP responsive element modulator (CREM) activator isoforms are involved in mammalian spermatogenesis and spermiogenesis. CREM proteins are highly expressed in postmeiotic germ cells of rodents and primates. Homozygous CREM inactivated mice exhibit round spermatid maturation arrest. The lack of CREM expression at both the mRNA and protein levels is associated with spermatid maturation arrest in infertile patients. Using real-time RT-PCR, we have examined the levels of CREM activator isoform mRNAs: CREMθ1, CREMθ2 and CREMt2 + Ex-γ in gametogenic and interstitial cell fractions from normal human testis, in homogenized tissue samples from spermatogenic arrest and from testicular tumors. We have shown for the first time the presence of CREM activator isoform containing exon γ (CREMτ2 + Exγ) in normal human spermatogenesis. Among the three CREM isoforms, CREMθ1 was expressed in its highest level in the male gonads. In comparison, CREMθ2 mRNA was significantly less suggesting that the P3 promoter is much more active in human testis than the P4 promoter. Minimal-nill levels of mRNA for either of the CREM activator isoforms were detected in lymphocytes or in gonadal tissues from patients with SCOS (Sertoli Cell Only Syndrome). This data underlines the significance of CREMθ1 isoform in the regulation of transcription during post-meiotic germ cell differentiation.

Dorota Fiszer

Małgorzata Białas

Natalia Rozwadowska

Włodzimierz Kosicki

Piotr Jedrzejczak

Maciej Kurpisz

Crem Activator Isoforms in Normal and Impaired Human Spermatogenesis Analyzed by Real Time RT-PCR

实时RT-PCR分析正常与受损人类精子发生中的Crem激活剂异构体

A German version of the Sexual Behaviour Questionnaire (SBQ-G) is a short self-rating instrument covering the four domains of sexual function using colloquial words. Frequency of sexual dysfunction was counted and chance-corrected retest reliability was calculated. Eleven women and 12 men participated. Reception of the SBQ-G was favourable and more females than males reported at least moderate sexual dysfunction. Sexual arousal and ability to enjoy sex showed the lowest retest stability. The SBQ-G is a recommendable clinical instrument with high practicability and satisfactory retest reliability. However, gender differences and a high variability of sexual functioning in healthy subjects should be kept in mind.

M. J. Müller

Development and Retest Reliability of a German Version of the Sexual Behaviour Questionnaire (SBQ-G)

《性行为问卷德文版（SBQ-G）的编制及重测信度研究》

Conventional semen analysis is rapidly losing its place as the gold standard of diagnosis and the cornerstone of treating the infertile male in modern times. Recent technology allows scientists to analyze sperm fertilizing potential and subsequent embryonic growth by studying factors that have previously escaped traditional parameters. It has become increasingly evident that nuclear DNA arrangement is essential to the fertilizing potential of sperm. A vast array of tests are now available to examine the genetic makeup of individual spermatozoa, ranging the entire gamut from simple bench top assays performed routinely to complex flow cytometric assays requiring highly-skilled technologists. Future research to compare these new tests to those more commonly in use, correlating them with reproductive outcome promises to fill the current void in the field of male infertility, paring innovative diagnostic (and prognostic) technological standards to the already existing sophisticated assortment of successful treatment modalities.

Dennis E. Marchesi

Huai L. Feng

Avner Hershlag

Current Assessment of Sperm DNA Integrity

精子DNA完整性的当前评估

In vitro maturation (IVM) of immature oocytes for infertile patients is an attractive treatment. It can avoid side effects of ovarian stimulation with gonadotropins. However, at the present the successful results of IVM treatment are lower than conventional in vitro fertilization (IVF) treatment. The key issue may be the IVM medium for immature oocyte maturation. In the present study, we compared 20% (v/v) human follicular fluid (hFF) and 20% (v/v) human umbilical cord serum (hUS) as a supplement to IVM medium. A total of 47 patients with polycystic ovary syndrome (PCOS) underwent 47 IVM treatment cycles. Immature oocytes (349) collected from 32 patients were cultured in IVM medium supplemented with hFF, and immature oocytes (160) collected from 15 patients were culture in IVM medium supplemented with hUS. The results indicate that the final maturation rate of oocytes cultured in IVM medium supplemented with hUS (93.8%) is significantly higher than those cultured in IVM medium supplemented with hFF (77.1%). The percentage of high-quality embryos produced from IVM medium supplemented with hUS (50.0%) is significantly higher than IVM medium supplemented with hFF (23.8%). In addition, the results also indicate that the final maturation rate of oocytes is higher in IVM medium supplemented with hUS and the time course of oocyte maturation is hastened. Following transfer 6 out of 15 patients (40.0%) become pregnant when IVM medium was supplemented with hUS, and 7 out of 31 patients (22.6%) were pregnant when IVM medium was supplemented with hFF. These results suggest that IVM medium containing hUS appears to be a more effective means to stimulate in vitro oocyte maturation and is capable of achieving a promising clinical outcome.

Z.-G. Zhang

J.-H. Zhao

Z.-L. Wei

L. Cong

P. Zhou

Y.-X. Cao

Human Umbilical Cord Blood Serum in Culture Medium on Oocyte Maturation<i>In Vitro</i>

人脐带血清在体外培养基中对卵母细胞成熟的影响

The effect of sample collection site on semen parameters in ten men aged between 22 and 24 years was investigated. Sperm was collected at two sites: in a university hospital restroom for general use and in a one-person hospital room. Samples were collected from the same individual twice, with an interval of two weeks between collections. Semen parameters for the two sites were compared. Samples were collected after a minimum of three days and not longer than seven days of sexual abstinence. Sperm concentration did not differ significantly between the university hospital restroom location (86.8 ± 25.4 × 106/ml; mean ± standard deviation) and the private hospital room (97.1 ± 72.0 × 106/ml). There was no difference in the total motile sperm count or daily sperm production between the collection sites. These results suggest that the collection site has little effect on semen parameters.

S. Oshio

M. Yotsukura

Y. Tohyama

M. Iwabuchi

Y. Adachi

Y. Ashizawa

H. Matsuda

T. Iiyama

H. Shimizu

H. Tomomasa

S. Yoshida

K. Takeda

T. Umeda

Effect of Sample Collection Site on Semen Parameters of Healthy Young Volunteers

健康年轻志愿者精液参数的样本采集部位影响

出版信息	出版商: Taylor and Francis Ltd.，出版周期: ，期刊类型: journal，开源期刊: 否
基本数据	创刊年份: 1996，原创研究文献占比: ，自引率:， Gold OA占比:

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Crem Activator Isoforms in Normal and Impaired Human Spermatogenesis Analyzed by Real Time RT-PCR

Development and Retest Reliability of a German Version of the Sexual Behaviour Questionnaire (SBQ-G)

Current Assessment of Sperm DNA Integrity

Human Umbilical Cord Blood Serum in Culture Medium on Oocyte MaturationIn Vitro

Effect of Sample Collection Site on Semen Parameters of Healthy Young Volunteers

Crem Activator Isoforms in Normal and Impaired Human Spermatogenesis Analyzed by Real Time RT-PCR

Development and Retest Reliability of a German Version of the Sexual Behaviour Questionnaire (SBQ-G)

Current Assessment of Sperm DNA Integrity

Human Umbilical Cord Blood Serum in Culture Medium on Oocyte MaturationIn Vitro

Effect of Sample Collection Site on Semen Parameters of Healthy Young Volunteers