Acta Biologica Hungarica provides a forum for original research works in the field of experimental biology. It covers cytology, functional morphology, embriology, genetics, endocrinology, cellular physiology, plant physiology, neurobiology, ethology and environmental biology with emphasis on toxicology. Publishes book reviews and advertisements.

Acta Biologica Hungarica is a forum for original research works in the field of experimental biology. It covers cytology, functional morphology, embryology, genetics, endocrinology, cellular physiology, plant physiology, neurobiology, ethology, and environmental biology, with a particular emphasis on toxicology. The journal also publishes book reviews and advertisements.

《Acta Biologica Hungarica》是一个专注于实验生物学领域的原创研究作品的论坛。期刊涵盖细胞学、功能形态学、胚胎学、遗传学、内分泌学、细胞生理学、植物生理学、神经生物学、动物行为学和环境生物学，特别强调毒理学。此外，期刊还会发布书评和广告。

Acta Biologica Hungarica

In the present study, the effect of ascorbic acid (5 mM) on some physiological parameters and three hormones (auxin, abscisic acid, salicylic acid) was determined under heat stress (40 °C) in maize tolerant cv. (MAY 69) and sensitive cv. SHEMAL (SH) at 0 h, 4 h and 8 h. Heat stress reduced total chlorophyll content (CHL), relative water content (RWC) and stomatal conductance ( g _s) in SH but did not lead to changes in MAY 69 at 4 h and 8 h. However, pretreatment with ascorbic acid increased (CHL), (RWC) and ( g _s) in SH under heat stress while it reduced MDA content significantly in both cv. We also observed that heat stress led to a reduction in SA level but increased ABA and IAA levels in SH, whereas it increased SA and IAA levels but did not change ABA level in MAY 69 at 4 h. Furthermore, in SH, ASC application under heat stress increased SA level and decreased IAA and ABA levels at 4 h, but it had no effect on SA and ABA at 8 h.

Burcu Dinler

Emel Demir

Yasemin Kompe

Regulation of auxin, abscisic acid and salicylic acid levels by ascorbate application under heat stress in sensitive and tolerant maize leaves

热胁迫下施用抗坏血酸对敏感和耐受玉米叶片中生长素、脱落酸和水杨酸水平调控的研究

The aim of the study was to analyse the effect of atmospheric cadmium (Cd) pollution of atmospheric origin in maize compared to a control without Cd pollution. The plant parameters investigated were the timing of phenological phases, leaf area index (LAI) and yield, while radiation and water regime parameters were represented by albedo (reflection grade) and evapotranspiration, respectively. In treatments with and without irrigation, Cd caused a significant reduction in LAI, accompanied by lower evapotranspiration. The mean annual albedo in the Cd-polluted treatment only rose to a moderate extent in 2011 (in 2010 there was hardly any change), but changes within the year were more pronounced in certain phases of development. Cd led to greater reflection of radiation by plants during the vegetative phase, so the radiation absorption of the canopy was reduced leading to a lower level of evapotranspiration. In the dry, hot year of 2011 maize plants in the non-irrigated treatments showed a substantial reduction in grain dry matter, but maize yield losses could be reduced by irrigation in areas exposed to Cd pollution.

Angéla Anda

Bernadett Illés

G. Soós

Effect of cadmium pollution of atmospheric origin on field-grown maize in two consecutive years with diverse weather conditions

大气来源镉污染对连续两年不同天气条件下田间种植玉米的影响

The present in vivo pot culture study showed hexavalent chromium (Cr^+6) induced phytotoxic impacts and its translocation potential i. 21 days old sesban ( Sesbania sesban L. Merrill.) seedlings. Cr^+6 showed significant growth retardation i. 21 days old sesban ( Sesbania sesban L. Merrill.) seedlings. Germination of seeds a. 10,000 mg L^-1 of Cr^+6 exhibi. 80% inhibition in germination. Seedling survival wa. 67% afte. 7 days of seedling exposure t. 300 mg kg^-1 of Cr^+6. Shoot phytotoxicity was enhanced fro. 6% t. 31% with elevated supply of Cr^+6 fro. 10 mg kg^-1 t. 300 mg kg^-1. Elevated supply of Cr^+6 exhibited increasing and decreasing trends in % phytotoxicity and seedling tolerance index, respectively. Elevated supply of chromium showed decreased chlorophyll and catalase activities. Peroxidase activities in roots and leaves were significantly higher at increased supply of Cr^+6. Cr bioconcentration in roots was nearl. 10 times more than stems whereas leaves showed nearly double accumulation than stems. Tissue specific chromium bioaccumulation showe. 53 an. 12 times more in roots and shoots respectively a. 300 mg kg^-1 Cr^+6 than control. The present study reveals potential of sesban for effective Cr translocation from roots to shoots as evident from their translocation factor and Total Accumulation Rate values.

Monalisa Mohanty

Chinmay Pradhan

Hemanta Patra

Chromium translocation, concentration and its phytotoxic impacts inin vivogrown seedlings ofSesbania sesbanL. Merrill.

铬在田间生长的大豆苗中的转运、浓度及其植物毒性影响。

Chitosan was obtained from cuticles of the housefly (Musca domestica) larvae. Antibacterial activities of different Mw chitosans were examined against six bacteria. Antibacterial mechanisms of chitosan were investigated by measuring permeability of bacterial cell membranes and observing integrity of bacterial cells. Results show that the antibacterial activity of chitosan decreased with increase in Mw. Chitosan showed higher antibacterial activity at low pH. Ca2+ and Mg2+ could markedly reduce the antibacterial activity of chitosan. The minimum inhibitory concentrations of chitosans ranged from 0.03% - 0.25% and varied with the type of bacteria and Mw of chitosan. Chitosan could cause leakage of cell contents of the bacteria and disrupt the cell wall.

Y J Jing

Y J Hao

H Qu

Y Shan

D S Li

R Q Du

Acta biologica Hungarica

Acta Biol Hung

Studies on the antibacterial activities and mechanisms of chitosan obtained from cuticles of housefly larvae.

家蝇幼虫几丁质抗菌活性及机制研究。

The housefly is an important resource insect and the housefly larvae are ideal source of food additives. The housefly larvae protein hydrolysates were obtained by enzymatic hydrolysis by alcalase and neutral proteinase. Their antioxidant activities were investigated, including the superoxide and hydroxyl radicalscavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, reducing power and metal chelating activity. The antioxidant activities of both hydrolysates increased with their increasing concentrations. The alcalase hydrolysate (AH) showed higher scavenging activities against hydroxyl radical and superoxide anion radical at low concentrations and higher metal-chelating activity than the neutral proteinase hydrolysate (NPH). The NPH exhibited higher scavenging activity against DPPH free radical and higher reducing power than the AH. Both hydrolysates showed more than 50% superoxide anion radical-scavenging activity at 10 μg/mL. These results indicate that both housefly larvae protein hydrolysates display high antioxidant activities and they could serve as potential natural antioxidant food additives.

Huan Zhang

Pan Wang

Ai-Jun Zhang

Xuan Li

Ji-Hong Zhang

Qi-Lian Qin

Yi-Jun Wu

Antioxidant activities of protein hydrolysates obtained from the housefly larvae

家蝇幼虫蛋白水解物的抗氧化活性

We aimed to examine the response of three tomato introgression lines (IL925.3, IL925.5 and IL925.6) to NaCl stress. These lines originated from a cross between M82 (Solanum lycopersicum) and the wild salttolerant tomato Solanum pennellii, each line containing a different fragment of the S.pennellii genome. Salt-sensitive phenotypes related to plant growth and physiology, and the response of antioxidants, pigments and antioxidant enzymes were measured. In general, salt stress decreased the fresh weight of leaves, leaf area and leaf number and an increase of Na+ accumulation in aerial parts was observed, which caused a reduction in the absorption of K+ and Ca2+. Salt stress also induced a decrease in chlorophyll, carotenoids and lipid peroxidation (MDA) and an increase in anthocyanins and reduced ascorbate, although some differences were seen between the lines, for example for carotenoid levels. Guaiacol peroxidase, catalase and glutathione reductase activity enhanced in aerial parts of the lines, but again some differences were seen between the three lines. It is concluded that IL925.5 might be the most sensitive line to salt stress as its dry weight loss was the greatest in response to salt and this line showed the highest Na+ ion accumulation in leaves.

Fedia Rebah

Chayma Ouhibi

K. H. Alamer

Najoua Msilini

Mouhiba Ben Nasri

Rebecca Stevens

Houneida Attia

Comparison of the responses to NaCl stress of three tomato introgression lines

三种番茄渐渗系对NaCl胁迫响应的比较

Traditional medicines are composed of herbal formulations and their active ingredients and constituents which play a crucial role in the treatment of various human ailments. Astragalus eremophilus and Melilotus indicus (L.) All. (syn. Melilotus parviflora Desf.) are used traditionally as antiperspirant, tonic, diuretic, laxative and narcotic agents. The current study was designed to investigate the Astragalus eremophilus and Melilotus indicus (L.) All. (syn. Melilotus parviflora Desf.) methanol extracts for their antioxidant, antibacterial and antifungal activities. Fine powder of A. eremophilus and M. parviflora was extracted with 70% methanol to get crude methanol extract. Extract was characterized for antioxidant, antibacterial and antifungal activities. Antioxidant activity of various concentrations (3 mg/ml, 1.5 mg/ ml, 0.75 mg/ml, and 0.38 mg/ml) of both plant extracts was analyzed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical. Salmonella typhemorium, Klebsiella pneumoniae (gram-negative) and Staphylococcus aureus, Enterococcus faecalis (gram-positive) bacterial strains were used for assessment of antibacterial activities. Antifungal activities of 7.5 mg/ml, 5.0 mg/ml, 2.5 mg/ml (A. eremophilus and M. parviflora) were conducted using Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Candida albicons. At high concentration (3 mg/ml), all the tested fractions of A. eremophilus and M. parviflora methanol extracts showed potent antioxidant activities, ranging between 83.8 and 63.33%. Antibacterial activities revealed that A. eremophilus showed a maximum zone of inhibition (8.1 ± 0.1) on Salmonella typhenorium followed by Enterococcus faecalis (7.2 ± 0.1), Klebsellesa pneumonia (6.1 ± 0.6), and Staphylococcus aureus (5.1 ± 0.4), and at highest concentration (7.5 mg/ml), however, maximum zone of inhibition of Melilotus parviflora was at 7.5 mg/ml followed by 5.0 mg/ml and 2.5 mg/ml against Klebsiella pneumonia, Staphylococcus aureus, Salmonella typhemorium and Enterococcus faecalis. Antifungal assessment of both plant extracts showed that the higher concentration (7.5 mg/ml) has significant inhibitory effect as compared to control. The results can lead to the conclusion that A. eremophilus and M. parviflora methanol extracts are indeed sources of potential therapeutic compounds against antibacterial, antifungal and free radical associated disorders.

Muhammad Nadeem Khan

Muhstaq Ahmed

Muhammad Wasim Khan

Rahmat Ali Khan

In vitro pharmacological effects of Astragalus eremophilus and Melilotus parviflora

Astragalus eremophilus 和 Melilotus parviflora 的体外药理作用

Drought is one the critical abiotic factors that reduces the germination, growth and yield of crops. Therefore the present project was designed with the objective to screen the best drought tolerant hybrid of Vigna radiata. Genetic variations for drought tolerance among these hybrids were assessed by simple and efficient technique. Seven hybrids of V. radiata (9801, 7002, 9706, 08003, 07007, 97012 and 08007) were used for screening against three levels of drought stress (zero, control), 5% (–0.05 MPa) and 10% (–0.1 MPa) induced by Polyethyleneglycol (PEG6000). A higher proline content was observed in 07007 (6.10 μg/g fresh weight) as compared to all other hybrids. Treated seedlings of each hybrid were compared with their respective control to evaluate the differences in their growth under drought. Different parameters such as percentage of germination, germination stress tolerance index (GSI), shoot and root weights (bath fresh and dry) and lengths, root length stress index (RLSI), dry matter stress index (DMSI) and plant height stress index (PHSI) showed considerable variations. Germination percentage, shoot weights, PHSI and DMSI decreased in all hybrids along with the increase of PEG induced drought stress (5% and 10% PEG). In contrary, root weights and RLSI were increased under drought. Overall 07007 showed a better performance, and can therefore be classified as a drought tolerant hybrid.

Shehzadi Saima

Guishuang Li

Guang Wu

Effects of drought stress on hybrids of Vigna radiata at germination stage

绿豆杂交种在萌发阶段干旱胁迫的影响

During the study of inhibition of amyloid fibril formation, α-chymotrypsin protein was developed in 55% ethanol at pH 7.0. We investigated the inhibitory effect of different spices on amyloid fibril formation using turbidity measurements and Congo red binding assays. We found that all spices except the black pepper and caraway seed prevented fibril formation. The highest inhibition was measured with the clove, which reduced the amount of aggregates by 90%. We studied the inhibitory effect of the cloves at different concentrations on aggregation, it was found that the inhibitory activity of clove is dependent on concentration. We have measured the total phenolic content of the spice extracts too. Based on all these findings we have come to the following conclusion: Our results indicate that spices can contain other compounds too – not only phenolic compounds – which influence the formation of amyloid fibrils, and the effectiveness of various phenolic compounds are different.

Márta Kotormán

Alexandra Varga

Phanindra Babu Kasi

János Nemcsók

Inhibition of the formation of amyloid-like fibrils with spices, especially cloves

香料，尤其是丁香对淀粉样纤维形成的抑制作用

The major aim of the present study was to investigate the influence of juglone (JU; 5-hydroxy-1,4-naphthoquinone) treatments on the expression level of Cat1, Cat2 and Cat3 genes, encoding the respective catalase isozymes in maize (Zea mays L.) and wheat (Triticum aestivum L.) seeds. In parallel, germination efficiency, catalase (CAT) activity and hydrogen peroxide (H2O2) content in juglone-exposed cereal seeds were assessed. Juglone applications significantly stimulated abundance of three target catalase transcripts as well as induced CAT activity and generation of H2O2 in both maize and wheat kernels. Furthermore, germination process of juglone-affected maize seeds was more severe suppressed than in case of wheat kernels. The role of juglone in triggering the oxidative stress as well as antioxidative responses in seeds of the studied model cereal species are discussed.

Hubert Sytykiewicz

Agnieszka Kozak

Bogumił Leszczyński

Cezary Sempruch

Iwona Łukasik

Iwona Sprawka

Katarzyna Kmieć

Monika Kurowska

Aldona Kopczyńska

Paweł Czerniewicz

Transcriptional profiling of catalase genes in juglone-treated seeds of maize (Zea maysL.) and wheat (Triticum aestivumL.)

过氧化氢酶基因在胡桃醌处理后的玉米（Zea mays L.）和小麦（Triticum aestivum L.）种子中的转录谱分析

Publication Information	Publisher: Akademiai Kiado，Publishing cycle: Quarterly，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 1983，Proportion of original research papers: 0.00%，Self Citation Rate:N.A.， Gold OA Rate: 0.00%
Average review cycle	网友分享经验：较慢,6-12周
Average recruitment ratio	网友分享经验：容易

Acta Biologica Hungarica

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