Acta Biochimica et Biophysica Hungarica

Specific staining on polyacrylamide gel of acetylcholinesterase (AchE) from brain and muscle of Heteropneustes fossilis showed one major tissue specific band in each. Eserine inhibited the enzyme competitively in both tissue homogenates. However, the normal level of activity and the pattern of the rate of inhibition with increasing eserine concentrations were different. Muscle showed higher AchE specific activity than brain. There was a hyperbolic increase in the percent of inhibition of AchE activity by eserine in muscle whereas in brain the pattern was biphasic. The apparent Km and Vmax in the two tissue homogenates were also different. The results suggest structural and functional variations of AchE in brain and muscle of H. fossilis.

Ratha Bk

Ramanujan Sn

Acta biochimica et biophysica Hungarica

Variations in acetylcholinesterase from brain and muscle of a freshwater air-breathing teleost, Heteropneustes fossilis.

淡水呼吸空气的辐鳍鱼类异齿鳗脑和肌肉中乙酰胆碱酯酶的变异。

Szent-Györgyi Ag

Acta Biochim Biophys Hung

Regulation of molluscan myosin by light chains.

软体动物肌球蛋白的光链调控。

An approach to ribosomal RNA turnover studies in which cytoplasmic RNA was extracted and subsequently fractionated to isolate ribosomal RNA is reported. The presumption that the pool of 28S and 18S RNAs represented ribosomal RNA, exclusively, proved false and led to erroneous results of ribosomal RNA turnover. Polyadenylated RNAs exhibited a heterogeneous size distribution and, although constituting only 3% (w/w) of the cytoplasmic RNA extract, accounted for fully 10% of radioactivity of the presumptive ribosomal RNA pool. Profiles from the radioactivity data suggested that the discrepant results were due to these polyadenylated RNAs. An additional analytical procedure, an oligo (dT) cellulose column chromatography of the RNA extract prior to the sucrose density gradient fractionation step, performed as described in this paper, proved an effective remedy for this error.

Onyezili Fn

Polyadenylated RNAs as error sources in ribosomal RNA turnover analyses.

多聚腺苷酸化RNA作为核糖体RNA周转分析中的错误来源。

出版信息	出版商: Akademiai Kiado，出版周期: ，期刊类型: journal，开源期刊: 否
基本数据	创刊年份: 1986，原创研究文献占比: ，自引率:， Gold OA占比:

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Variations in acetylcholinesterase from brain and muscle of a freshwater air-breathing teleost, Heteropneustes fossilis.

Regulation of molluscan myosin by light chains.

Polyadenylated RNAs as error sources in ribosomal RNA turnover analyses.

Polyadenylated RNAs as error sources in ribosomal RNA turnover analyses.

Regulation of molluscan myosin by light chains.

Variations in acetylcholinesterase from brain and muscle of a freshwater air-breathing teleost, Heteropneustes fossilis.