Clinical Reproduction and Fertility

Healy Dl

Clinical reproduction and fertility

Clin Reprod Fertil

Clinical status of antiprogesterone steroids.

抗孕酮类固醇的临床状况。

Concentrations of pregnancy-associated plasma protein-A (PAPP-A), human chorionic gonadotrophin (hCG) and pregnancy specific beta 1-glycoprotein (SP1) were measured by radioimmunoassay in 164 serum and 29 tissue samples obtained from 87 non-pregnant and 29 pregnant women with either tubal (n = 16) or intrauterine (n = 13) pregnancies. Of the 47 serum samples obtained from patients with a tubal pregnancy only two were positive for PAPP-A, whereas in excess of 94% of the samples were positive for both hCG and SP1. Serial measurements of hCG, and SP1, demonstrated an apparently normal growth rate for the tubally implanted trophoblast for the first 6 weeks of pregnancy. Thereafter, the tubal trophoblast is compromised since the tissue content of PAPP-A and hCG was significantly lower than that in intra-uterine derived trophoblastic tissue. By contrast, the content of both PAPP-A and hCG was significantly greater in the trophoblastic, both intra or extra-uterine derived, than in the endometrial/decidual, tissue. Furthermore, the absence of immunoreactive PAPP-A and the severely depressed circulating levels of PAPP-A in women with tubal pregnancies can be attributed to diminished secretion of PAPP-A into the maternal circulation. Therefore, in conjunction with a positive pregnancy test and the patient's clinical history, a severely depressed or absent serum PAPP-A level may aid in the diagnosis of extrauterine pregnancy.

Sinosich Mj

Ferrier A

Teisner B

Porter R

Westergaard Jg

Saunders Dm

Grudzinskas Jg

Circulating and tissue concentrations of pregnancy-associated plasma protein-A (PAPP-A) in tubal ectopic gestation.

输卵管异位妊娠中妊娠相关血浆蛋白-A（PAPP-A）的循环和组织浓度。

Jones Wr

Occasional comment. Some immunological considerations in spontaneous and recurrent abortion.

偶发性评论。自发性与复发性流产中的一些免疫学考量。

The immunobead test is excellent for the routine screening of semen for sperm antibodies but the polyacrylamide beads currently used are of variable diameter (3-10 micron) which tends to obscure details of regional binding. Thus the use of smaller latex beads of more uniform diameter has been investigated. Latex beads were coated with IgG by exposure to anti D serum for a mixed antiglobulin reaction (MAR). Rabbit antihuman IgG and IgA antibodies were covalently bound to carboxylated latex microspheres (CLM) by the carbodiimide reaction. Polyacrylamide immunobeads (IBT) with antihuman IgG or IgA antibodies covalently attached were obtained commercially. The IgG IBT and MAR were compared in 55 samples. The MAR was positive in 15 of the 31 IBT positive samples but the proportion of sperm with attached beads was lower. CLM and IBT were compared in 106 samples. Both tests were positive in 34 samples with virtually identical proportions of motile sperm with beads attached and the patterns of attachment were similar. These results show that CLM microspheres give equivalent results to IBT and that as they are smaller and have a more uniform size they provide advantage in ease of detection of positive reactions and in determination of the regional blinding pattern.

MacMillan Ra

Baker Hw

Comparison of latex and polyacrylamide beads for detecting sperm antibodies.

乳胶与聚丙烯酰胺珠检测精子抗体的比较。

Publication Information	Publisher: CSIRO，Publishing cycle: ，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 1985，Proportion of original research papers: ，Self Citation Rate:， Gold OA Rate:

Clinical Reproduction and Fertility

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Clinical status of antiprogesterone steroids.

Circulating and tissue concentrations of pregnancy-associated plasma protein-A (PAPP-A) in tubal ectopic gestation.

Occasional comment. Some immunological considerations in spontaneous and recurrent abortion.

Comparison of latex and polyacrylamide beads for detecting sperm antibodies.

Comparison of latex and polyacrylamide beads for detecting sperm antibodies.

Occasional comment. Some immunological considerations in spontaneous and recurrent abortion.

Clinical status of antiprogesterone steroids.

Circulating and tissue concentrations of pregnancy-associated plasma protein-A (PAPP-A) in tubal ectopic gestation.