酵母发表关于单细胞真菌研究最重要进展的原创文章和评论，包括广泛适用的创新方法。对于那些希望跟上这个快速发展的酵母生物学领域的人来说，这是一本必不可少的读物。涵盖的主题包括：生物化学和分子生物学；生物多样性和分类学；生物技术；细胞和发育生物学；生态与进化；遗传学和基因组学；新陈代谢和生理学；病理学;合成和系统生物学；工具和资源

The journal 'Yeast' publishes original articles and reviews on significant advancements in single-cell fungal research, including widely applicable innovative methods. It is an essential read for those looking to keep up with the rapidly evolving field of yeast biology. Topics covered include biochemistry and molecular biology, biodiversity and taxonomy, biotechnology, cell and developmental biology, ecology and evolution, genetics and genomics, metabolism and physiology, pathology, synthetic and systems biology, as well as tools and resources.

《Yeast》期刊发表关于单细胞真菌研究的重要原创文章和评论，涵盖广泛适用的创新方法。对于希望跟上这一快速发展的酵母生物学领域的研究者来说，这是一本必不可少的读物。期刊涉及的主题包括生物化学与分子生物学、生物多样性与分类学、生物技术、细胞与发育生物学、生态与进化、遗传学与基因组学、新陈代谢与生理学、病理学、合成与系统生物学，以及相关工具和资源。

Yeast

The growing trend in the wine industry is the revaluation of the role of non-Saccharomyces yeasts, promoting the use of these yeasts in association with Saccharomyces cerevisiae. Non-Saccharomyces yeasts contribute to improve wine complexity and organoleptic composition. However, the use of mixed starters needs to better understand the effect of the interaction between these species during alcoholic fermentation. The aim of this study is to evaluate the influence of mixed starter cultures, composed by combination of different S. cerevisiae and Hanseniaspora uvarum strains, on wine characteristics and to investigate the role of cell-to-cell contact on the metabolites produced during alcoholic fermentation. In the first step, three H. uvarum and two S. cerevisiae strains, previously selected, were tested during mixed fermentations in natural red grape must in order to evaluate yeast population dynamics during inoculated fermentation and influence of mixed starter cultures on wine quality. One selected mixed starter was tested in a double-compartment fermentor in order to compare mixed inoculations of S. cerevisiae/H. uvarum with and without physical separation. Our results revealed that physical contact between S. cerevisiae and H. uvarum affected the viability of H. uvarum strain, influencing also the metabolic behaviour of the strains. Although different researches are available on the role of cell-to-cell contact-mediated interactions on cell viability of the strains included in the mixed starter, to our knowledge, very few studies have evaluated the influence of cell-to-cell contact on the chemical characteristics of wine.

Angela Pietrafesa

Angela Capece

Rocchina Pietrafesa

Marina Bely

Patrizia Romano

<scp>Saccharomyces cerevisiae</scp>and<scp>Hanseniaspora uvarum</scp>mixed starter cultures: Influence of microbial/physical interactions on wine characteristics

<scp>酿酒酵母</scp>与<scp>汉森酵母</scp>混合发酵剂：微生物/物理相互作用对葡萄酒特性的影响

A novel series of plasmid vectors named pDUAL have been developed. These vectors enable one to introduce not only multicopies of genes with episomal maintenance but also a single copy with chromosomal integration into the fission yeast, Schizosaccharomyces pombe. The multicopy plasmids can be easily converted to fragments for chromosomal integration by digestion of the plasmids with a certain restriction endonuclease before transformation of the yeast cells. The resultant fragments, lacking the autonomously replicating sequence, are designed for targeting into the chromosomal leu1 locus by homologous recombination. Whether the transformants are the results of episomal maintenance of the plasmid or homologous gene targeting can be readily checked by their requirement for uracil or leucine, or by the PCR diagnostic analysis. Furthermore, we propose the use of pDUAL derivatives for PCR-based chromosomal tagging of a gene to introduce several tags into 5'-terminus of a gene, employing a set of primers. Using these all-in-one vectors, a suitable mode of expression of a cloned gene can be selected for individual analysis without any complicated subcloning processes.

Akihisa Matsuyama

Atsuko Shirai

Yoko Yashiroda

Ayako Kamata

Sueharu Horinouchi

Minoru Yoshida

pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast

pDUAL，一种适用于多种用途、多拷贝的载体，能够在裂殖酵母中进行染色体整合

Abstract At Chx17p is a putative K + /H + exchanger from Arabidopsis thaliana , expressed in the roots and probably involved in K + acquisition and homeostasis. AtCHX17 cDNA complements the phenotypes of the kha1 Δ mutation in S. cerevisiae cells: a growth defect at increased pH and hygromycin sensitivity. The localization of GFP‐tagged At Chx17 protein in yeast cells is similar to that of Sc Kha1p: a bold dotted pattern inside the cells resembling the Golgi fluorescence markers. These results show that (a) the proteins At Chx17 and Sc Kha1 could have similar functions and (b) S. cerevisiae kha1 deletion mutants could serve for the heterologous expression and characterization of plant transporters. The results of this work are evidence that a S. cerevisiae strain with deletions of genes encoding alkali–metal–cation/H + antiporters (i.e. Nha1p, Nhx1p, Kha1p) could be an ideal tool for expression and functional analysis of any type of similar plant antiporters (plasma membrane, endosomal/prevacuolar and Golgi). Copyright © 2006 John Wiley &amp; Sons, Ltd.

Lydie Maresova

Hana Sychrova

Arabidopsis thaliana CHX17 gene complements the kha1 deletion phenotypes in Saccharomyces cerevisiae

拟南芥CHX17基因补全了酿酒酵母kha1缺失表型

Abstract The understanding of the controlling factors of calcium homeostasis in Aspergillus fumigatus is very poor, although this ion is involved in several important events of these particular cells. We have cloned, identified and expressed for functional complementation a PMR1‐like Ca 2+ ‐ATPase gene from A. fumigatus . The Afpmr1 gene encodes a protein of 1061 deduced amino acids, containing all the conserved subdomains found in other P‐type ATPases: the phosphatase region, phosphorylation site, FITC labelling site, ATP binding domain; E 386 , N 871 , D 875 amino acid residues for calcium ion interaction and Q 880 , a residue that alters ion selectivity in PMR1. The expressed Af PMR1 in S. cerevisiae K616 strain functionally complemented the deficient growth in EGTA (5–20 m M )‐ and MnCl 2 (4 m M )‐containing medium. These results demonstrate the first evidence of a Ca 2+ ‐ATPase in A. fumigatus and strongly suggest a role for this enzyme in calcium and manganese homeostasis. Copyright © 2005 John Wiley &amp; Sons, Ltd.

F. M. Soriani

V. P. Martins

T. Magnani

V. G. Tudella

C. Curti

S. A. Uyemura

A PMR1‐like calcium ATPase of Aspergillus fumigatus: cloning, identification and functional expression in S. cerevisiae

烟曲霉中一种类似PMR1的钙ATP酶：克隆、鉴定及在酿酒酵母中的功能表达

Abstract Reporter proteins are essential tools in the study of biological processes and are employed to monitor changes in gene expression and protein levels. Luciferases are reporter proteins that enable rapid and highly sensitive detection with an outstanding dynamic range. Here we evaluated the usefulness of the 19 kDa luciferase NanoLuc (Nluc), derived from the deep sea shrimp Oplophorus gracilirostris , as a reporter protein in yeast. Cassettes with codon‐optimized genes expressing yeast Nluc (yNluc) or its destabilized derivative yNlucPEST have been assembled in the context of the dominant drug resistance marker kanMX. The reporter proteins do not impair the growth of yeast cells and exhibit half‐lives of 40 and 5 min, respectively. The commercial substrate Nano‐Glo® is compatible with detection of yNluc bioluminescence in &lt; 50 cells. Using the unstable yNlucPEST to report on the rapid and transient expression of a heat‐shock promoter (P CYC1–HSE ), we found a close match between the intensity of the bioluminescent signal and mRNA levels during both induction and decay. We demonstrated that the bioluminescence of yNluc fused to the C‐terminus of a temperature‐sensitive protein reports on its protein levels. In conclusion, yNluc and yNlucPEST are valuable new reporter proteins suitable for experiments with yeast using standard commercial substrate. © 2016 The Authors. Yeast published by John Wiley &amp; Sons Ltd.

Anna E. Masser

Ganapathi Kandasamy

Jayasankar Mohanakrishnan Kaimal

Claes Andréasson

Luciferase NanoLuc as a reporter for gene expression and protein levels in Saccharomyces cerevisiae

用于酿酒酵母基因表达和蛋白质水平检测的纳米荧光素酶报告系统

ABSTRACT Biodiversity gaps in microorganisms, such as yeasts, blur our understanding of microbial diversity, introducing biases in their biogeography, ecology, and taxonomy. The genus Carlosrosaea is a potential plant growth booster, yet it is still a little‐known yeast group. Considering that databases like GBIF and GenBank are powerful tools for exploring biodiversity data, we aimed to map the geographic distribution, ecological patterns, and taxonomic potential of the genus Carlosrosaea . We found 176 records of the genus, with about 70% associated with plant material, mostly leaves. Furthermore, 55% of the records pertained to the tropical region and only 12% to the temperate. The data indicates the existence of more than a dozen possible new species of the genus, cataloged yet undescribed. This study advances our understanding of the geographic, ecological, and taxonomic aspects of Carlosrosaea . It also highlights how public databases and literature reviews provide accessible ways to analyze information about microbial groups with limited data.

Ciro Ramon Félix

Hector M. C. Navarro

Melissa Fontes Landell

The Hidden Global Diversity of the Yeast Genus Carlosrosaea: A Biodiversity Databases Perspective

酵母属Carlosrosaea的隐秘全球多样性：生物多样性数据库视角

ABSTRACT The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isolation of H. pylori and yeast, rapid urease test (RUT), staining with Gram's and hematoxylin &amp; eosin (H&amp;E), and immunohistochemistry (IHC) methods. In the other three groups, DNAs extracted from H. pylori and yeasts were used for the amplification of H. pylori ‐specific genes. Wet mounts of yeasts in Group 2 were examined to observe intracellular bacteria and released EVs. Among 221 patients, 65 (29.3%) had oral yeast, 35 (15.8%) H. pylori , and 31 (14%) gastric yeast. Culture of oral yeasts showed a significant correlation with the detection of H. pylori by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&amp;E (4.9%), and culture (4%) ( p &lt; 0.05). Gram‐stained biopsies showed the occurrence of yeast and H. pylori , and the release of EVs from yeast. Detection of similar H. pylori genes in oral and gastric yeasts from patients in Group 2 showed their common source. Oral yeasts in Groups 3 and 4 also carried H. pylori genes. Wet mount preparations of yeasts showed intracellular bacteria inside the yeast vacuole and the release of EVs that could carry H. pylori . Oral yeast protects its intracellular H. pylori and releases it inside EVs to safely reach gastric mucosa. Yeast, as the environmental reservoir of H. pylori , plays a crucial role in bacterial reinfection after successful eradication.

Sara Kadkhodaei

Atousa Hatefi

Shahrzad Pedramnia

Elham Godini

Saman Khalili‐Samani

Parastoo Saniee

Abdolfattah Sarrafnejad

Ali‐Hatef Salmanian

Masoud Sotoudeh

David Y. Graham

Reza Malekzadeh

Farideh Siavoshi

Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori

口腔酵母在补充酵母和幽门螺杆菌胃黏膜中的作用

ABSTRACT Saccharomyces cerevisiae is an excellent model to study the effect of external cues on cell division and stress response. 5‐Fluorocuracil (5‐FU) has been used to treat solid tumors since several decades. The drug was initially designed to interfere with DNA replication but was later found to exert its antiproliferative effect also via RNA‐dependent processes. Since 5‐FU inhibits the activity of the 3′−5′‐exoribonuclease Rrp6 in yeast and mammals, earlier work has compared the effect of 5‐FU treatment and RRP6 deletion at the transcriptome level in diploid synchronized yeast cells. To facilitate interpreting the expression data we have developed an improved 5‐F luoro u racil R NA (5‐FU R ) expression viewer. Users can access information via genome coordinates and systematic or standard names for mRNAs and Xrn1‐dependent‐, stable‐, cryptic‐, and meiotic unannotated transcripts (XUTs, SUTs, CUTs, and MUTs). Normalized log2‐transformed or linear data can be displayed as filled diagrams, line graphs or color‐coded heatmaps. The expression data are useful for researchers interested in processes such as cell cycle regulation, mitotic repression of meiotic genes, the effect of 5‐FU treatment and Rrp6 deficiency on the transcriptome and expression profiles of sense/antisense loci that encode overlapping transcripts. The viewer is accessible at http://5fur.genouest.org .

Ugo Szachnowski

Oliver Sallou

Mateo Boudet

Anthony Bretaudeau

Maxime Wery

Antonin Morillon

Michael Primig

The 5‐Fluorouracil RNA Expression Viewer (5‐FUR) Facilitates Interpreting the Effects of Drug Treatment and RRP6 Deletion on the Transcriptional Landscape in Yeast

5‐氟尿嘧啶RNA表达查看器（5‐FUR）有助于解释药物处理和RRP6缺失对酵母转录组景观的影响

ABSTRACT Yeasts in the subphylum Saccharomycotina are found across the globe in disparate ecosystems. A major aim of yeast research is to understand the diversity and evolution of ecological traits, such as carbon metabolic breadth, insect association, and cactophily. This includes studying aspects of ecological traits like genetic architecture or association with other phenotypic traits. Genomic resources in the Saccharomycotina have grown rapidly. Ecological data, however, are still limited for many species, especially those only known from species descriptions where usually only a limited number of strains are studied. Moreover, ecological information is recorded in natural language format limiting high throughput computational analysis. To address these limitations, we developed an ontological framework for the analysis of yeast ecology. A total of 1,088 yeast strains were added to the Ontology of Yeast Environments (OYE) and analyzed in a machine‐learning framework to connect genotype to ecology. This framework is flexible and can be extended to additional isolates, species, or environmental sequencing data. Widespread adoption of OYE would greatly aid the study of macroecology in the Saccharomycotina subphylum.

Marie‐Claire Harrison

Dana A. Opulente

John F. Wolters

Xing‐Xing Shen

Xiaofan Zhou

Marizeth Groenewald

Chris Todd Hittinger

Antonis Rokas

Abigail Leavitt LaBella

Exploring Saccharomycotina Yeast Ecology Through an Ecological Ontology Framework

通过生态本体框架探索酵母菌生态学

ABSTRACT Engineering the glycerol‐3‐phosphate pathway could enhance erythritol production by accelerating glycerol uptake. However, little work has been conducted on the alternative dihydroxyacetone (DHA) pathway in Yarrowia lipolytica . Herein, this route was identified and characterized in Y. lipolytica by metabolomic and transcriptomic analysis. Moreover, the reaction catalyzed by dihydroxyacetone kinase encoded by dak2 was identified as the rate‐limiting step. By combining NHEJ‐mediated insertion mutagenesis with a push‐and‐pull strategy, Y. lipolytica strains with high‐yield erythritol synthesis from glycerol were obtained. Screening of a library of insertion mutants allows the identification of a mutant with fourfold increased erythritol production. Overexpression of DAK2 and glycerol dehydrogenase GCY3 together with gene encoding transketolase and transaldolase from the nonoxidative part of the pentose phosphate pathway led to a strain with further increased productivity with a titer of 53.1 g/L and a yield 0.56 g/g glycerol, which were 8.1‐ and 4.2‐fold of starting strain.

Feng Liu

Jing‐Tao Tian

Ya‐Ting Wang

Lingxuan Zhao

Zhijie Liu

Jun Chen

Liu‐Jing Wei

Patrick Fickers

Qiang Hua

Improving an Alternative Glycerol Catabolism Pathway in Yarrowia lipolytica to Enhance Erythritol Production

改进Yarrowia lipolytica中替代甘油分解代谢途径以提高赤藓糖醇产量

Abbreviation	Yeast.
Journal Impact	2.27
Quartiles(Global)	MYCOLOGY(Q3)

ISSN	0749-503X, 1097-0061
h-index	111

Publication Information	Publisher: John Wiley and Sons Ltd，Publishing cycle: Monthly，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 1985，Proportion of original research papers: 76.67%，Self Citation Rate:9.10%， Gold OA Rate: 25.78%
Average review cycle	网友分享经验：平均3.0个月
Average recruitment ratio	网友分享经验：较易

Yeast

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Saccharomyces cerevisiaeandHanseniaspora uvarummixed starter cultures: Influence of microbial/physical interactions on wine characteristics