Banko Janakari

Mitra Pathak

S. K. Kasaju

Short article Euphorbia umbellata, a newly recorded naturalized species from Nepal

短文：大戟属新归化种——尼泊尔新记录的伞形大戟

Wood anatomical characters were investigated for Juniperus squamata Buch.-Ham. ex. D. Don from high mountains of Trans-Himalayan Zone of Manang District of Central Nepal. We studied the anatomical features and the inter-relationship between the anatomical parameters of Himalayan Juniper from 30 different wood samples collected at 4600 m above the mean sea level (msl). Wood samples were boiled at 100ºC in oven, and sectioning was done using the KD-3390 Semi-automatic Microtome. The sections were then dehydrated in alcohol stained with 1% Safranin and fast green solutions, and permanent slides were prepared and observed under microscope. J. squamata is a softwood species and is characterized by the presence of distinct narrow annual growth rings with gradual to abrupt transition from earlywood to latewood. Both earlywood and latewood tracheids comprising square to polygonal cells, circular bordered pits and few resin cells arranged in loose tangential bands. The rays were found to be exclusively uniseriate and homogenous; most of the ray cells contained prismatic crystals while cupressoid pits were present in the ray cells.The annual-ring-width showed a positive correlation with both the earlywood and latewood width but a negative correlation with tracheids length. In softwood species like Junipers, tracheid length is an important characteristic, not only for wood and fiber quality, but also for the tree's hydraulic architecture. Furthermore, this is also coupled with the acclimatization of the species in harsh climatic condition of the arid trans-Himalayan region. Dwarf individuals with reduced growth ring dimensions and increased tracheid length ensure effective water transportation towards the shoot system. Therefore, this intra-specific variation in wood anatomical features of J.squamata is due to variation in micro-habitat types.

Pratikshya Chalise

Achyut Tiwari

Wood anatomical features of Juniperus squamata Buch.-Ham. ex. D. Don from high mountains of Trans-Himalayan Zone of central Nepal

尼泊尔中部喜马拉雅山脉地区高山上的刺柏属植物Juniperus squamata Buch.-Ham. ex. D. Don的木材解剖特征

Kiran Kumar Pokharel

Rajendra KC

Enhancing the Effectiveness of Nepal’s Participation in Multilateral Environmental Agreements

增强尼泊尔参与多边环境协定的有效性

This research aims to develop a protocol for inducing somatic embryogenesis and plant regeneration through callus in Piper longum. Leaf and nodal explants were cultured in the Murashige and Skoog medium added with 2, 4-dichlorophenoxyacetic acid (2, 4-D) or α-naphthaleneacetic acid (NAA), Kinetin (KN), and 10% coconut water (CW). The maximum frequency of embryogenic/nodular callus development (66.66%) and the number of embryos (28.33±3.511) per 0.2-0.3 g embryogenic callus developed from the leaf explant was obtained in the MS + 1.5 mg/L 2, 4-D + 1.0 mg/L KN + 10% CW. Similarly, the maximum frequency of embryogenic/nodular callus production (50%) and the number of embryos (12.66±2.51) per 0.2-0.3 g embryogenic callus developed from the stem explant were obtained in the MS + 1.0 mg/L NAA + 10% CW. Somatic embryo differentiation, maturation, and conversion were obtained when the nodular calli with various stages of embryos were transferred to the MS + 0.25 -1.5 mg/L thidiazuron + 10% CW. Somatic embryos were also transformed into seedlings after being transplanted to the MS medium with no growth regulators. This study developed a technique for micropropagation of P. longum using somatic embryos derived from leaf and nodal explants, which could serve as the foundation for an alternate method of micropropagation and ex-situ germplasm conservation.

Chandra Bahadur Thapa

Krishna Kumar Pant

Hari Datta Bhattarai

Bijaya Pant

Indirect somatic embryogenesis and plant regeneration through leaf and nodal cultures of Piper longum L.

胡椒（Piper longum L.）叶片和节段培养中的间接体胚发生与植株再生

Hyperaccumulator plants, such as Ipomoea carnea, are known for their ability to accumulate heavy metals in their aerial parts. This study aimed to determine whether I. carnea is an iron hyperaccumulator. Ten sites within the Ramgram Municipality, Nawalparasi (west) district were selected randomly- five near industrial areas and five away from industries. Two plants from each site were harvested along with the soil samples nearby, following the standard protocols for collection. After sun-drying, the plant samples for four weeks and soil samples for three days, iron concentrations in roots, shoots, leaves, and soil were measured with an atomic absorption spectrophotometer using ashing and wet acid digestion. The results showed a significant correlation (r = 0.728, p &lt; 0.05) between the root iron concentration and the total iron in the plant. The analysis of variance revealed differences in iron accumulation in the roots, stems, and leaves of I. carnea. In the industrial areas, the biological absorption coefficient was 0.12 as compared to 0.08 in the non-industrial areas, indicating I. carnea is a low accumulator. The bioconcentration factor and the translocation factor were observed to be higher in the industrial areas. Overall, I. carnea was not found to be an effective iron hyperaccumulator in the study area, as indicated by the biological absorption coefficient, bio-concentration factor, and translocation factor values. In addition, the soil iron concentration was within the acceptable limit in the study area.

Suyog Raj Regmi

Balkrishna Ghimire

Evaluating the potentiality of naturally growing Ipomoea carnea Jacq. as an iron hyperaccumulator in Ramgram Municipality, Nawalparasi (west), Nepal

评估尼泊尔纳瓦帕拉西（西部）拉姆格拉姆自治市自然生长的三裂叶薯（Ipomoea carnea Jacq.）作为铁超富集植物的潜力

Publication Information	Publisher: Forest Research and Training Centre (FRTC)，Publishing cycle: ，Journal Type: journal，Open Access Journals: Yes
Basic data	Year of publication: 2019，Proportion of original research papers: ，Self Citation Rate:， Gold OA Rate:
Average review cycle	8 weeks

Banko Janakari

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Short article Euphorbia umbellata, a newly recorded naturalized species from Nepal

Wood anatomical features of Juniperus squamata Buch.-Ham. ex. D. Don from high mountains of Trans-Himalayan Zone of central Nepal

Enhancing the Effectiveness of Nepal’s Participation in Multilateral Environmental Agreements

Indirect somatic embryogenesis and plant regeneration through leaf and nodal cultures of Piper longum L.

Evaluating the potentiality of naturally growing Ipomoea carnea Jacq. as an iron hyperaccumulator in Ramgram Municipality, Nawalparasi (west), Nepal

Short article Euphorbia umbellata, a newly recorded naturalized species from Nepal

Wood anatomical features of Juniperus squamata Buch.-Ham. ex. D. Don from high mountains of Trans-Himalayan Zone of central Nepal

Enhancing the Effectiveness of Nepal’s Participation in Multilateral Environmental Agreements

Indirect somatic embryogenesis and plant regeneration through leaf and nodal cultures of Piper longum L.

Evaluating the potentiality of naturally growing Ipomoea carnea Jacq. as an iron hyperaccumulator in Ramgram Municipality, Nawalparasi (west), Nepal

ISSN	1016-0582, 2631-2301
h-index	4