Journal of Microcolumn Separations

Journal of Microcolumn SeparationsVolume 13, Issue 6 p. 255-256 Microcolumn abstracts Douglas E. Raynie, Douglas E. Raynie The Procter & Gamble Company, Miami Valley Laboratories, P. O. Box 538707, Cincinnati, OH 45253-8707, USASearch for more papers by this author Douglas E. Raynie, Douglas E. Raynie The Procter & Gamble Company, Miami Valley Laboratories, P. O. Box 538707, Cincinnati, OH 45253-8707, USASearch for more papers by this author First published: 16 October 2001 https://doi.org/10.1002/mcs.1051AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume13, Issue62001Pages 255-256 RelatedInformation

Douglas E. Raynie

Microcolumn abstracts

微柱摘要

Capillary electrochromatography has been coupled to an ion trap mass spectrometer via a sheath flow electrospray interface. Peptide analysis was performed on 25 cm columns packed with C18-bonded silica particles, and very fast separations were obtained. Full mass spectrometry and tandem mass spectrometry (MS/MS) detection were evaluated in terms of sensitivity and scanning speed. The separation of seven selected peptides and their detection by MS/MS in selected reaction monitoring mode was demonstrated. The performance of this sheath flow interface also was compared with the previously reported sheathless interface. Despite the loss in sensitivity (20–40-fold), the sheath flow interface was shown to be superior in terms of ruggedness, and allowed the use of higher electric fields to achieve faster analysis times. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 243–249, 2001

Marco Gaspari

Marjan Guček

Karin Walhagen

Rob J. Vreeken

Elwin R. Verheij

Ubbo R. Tjaden

Jan Van der Greef

Ion trap mass spectrometry as detector for capillary electrochromatography of peptides: Possibilities and limitations

离子阱质谱作为肽毛细管电色谱检测器的潜力与局限

Journal of Microcolumn SeparationsVolume 13, Issue 4 p. 176-177 Microcolumn abstracts Douglas E. Raynie, Douglas E. Raynie The Procter & Gamble Company, Miami Valley Laboratories, P. O. Box 538707, Cincinnati, OH 45253-8707, USASearch for more papers by this author Douglas E. Raynie, Douglas E. Raynie The Procter & Gamble Company, Miami Valley Laboratories, P. O. Box 538707, Cincinnati, OH 45253-8707, USASearch for more papers by this author First published: 02 July 2001 https://doi.org/10.1002/mcs.1038AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume13, Issue42001Pages 176-177 RelatedInformation

微柱文摘

Journal of Microcolumn SeparationsVolume 13, Issue 6 p. 255-256 Microcolumn abstracts Douglas E. Raynie, Douglas E. Raynie The Procter & Gamble Company, Miami Valley Laboratories, P. O. Box 538707, Cincinnati, OH 45253-8707, USASearch for more papers by this author Douglas E. Raynie, Douglas E. Raynie The Procter & Gamble Company, Miami Valley Laboratories, P. O. Box 538707, Cincinnati, OH 45253-8707, USASearch for more papers by this author First published: 16 October 2001 https://doi.org/10.1002/mcs.1051AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume13, Issue62001Pages 255-256 RelatedInformation

Abstract A new gradient system for CEC was designed and adapted to a home‐built CEC system. Chromatographic separations are initiated with a predetermined mobile phase composition and, after a desired time period, a syringe pump delivers pure organic solvent at a preset flow rate into a 2 mL vial used as the inlet buffer reservoir. Mixing is achieved by the use of a microstir bar in the buffer reservoir, which in turn is driven by an air‐actuated stir plate. In this way, a convex‐shaped gradient is generated, and the mobile phase is introduced into the separation column by electro‐osmotic flow. Use of this system significantly reduced analysis time while still retaining acceptable reproducibility. Nonaqueous CEC was used for isocratic and gradient separation (&lt;3.5% RSD in retention times). Demonstrated applications include the analysis of triglycerides in different vegetable oil samples. This system is expected to be well suited for generating other types of gradients, such as pH and ionic strength gradients, in electroseparation techniques. © 2001 John Wiley &amp; Sons, Inc. J Micro Sep 13: 351–360, 2001

Juan Carlos Medina

M. Carmen Alonso

Damia Barcelo

Milton L. Lee

Simple gradient system For capillary electrochromatography

毛细管电色谱的简单梯度系统

Retention of nonporous micron-sized silica gel particles in gravitational field-flow fractionation (GFFF) was investigated with respect to the activity of the hydrodynamic lift-forces and surface forces. The hydrodynamic lift-forces act near the channel wall in the same direction as repulsive electrostatic forces against the gravity so that the particles are focused. Although a complete quantitative description of the hydrodynamic lift-forces, which would enable prediction of the retention time, is not yet available, the experiments show that the magnitude of both forces is comparable with the particle effective weight and that the forces can be used for efficient separation of micron-sized particles. Higher ionic strengths of the carrier liquid attained by addition of NaCl decrease the repulsive electrostatic force and dramatically influence retention of the particles, yet to be reported in GFFF. © 1996 John Wiley & Sons, Inc.

Jiří Pazourek

Karl-Gustav Wahlund

Josef Chmelík

Effect of flowrate and ionic strength on retention of nonporous micron-sized silica gel particles in gravitational field-flow fractionation

流量和离子强度对非孔微米级硅胶颗粒在重力场流分级中保留的影响

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Journal of Microcolumn Separations

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Microcolumn abstracts

Ion trap mass spectrometry as detector for capillary electrochromatography of peptides: Possibilities and limitations

Microcolumn abstracts

Microcolumn abstracts

Simple gradient system For capillary electrochromatography

Effect of flowrate and ionic strength on retention of nonporous micron-sized silica gel particles in gravitational field-flow fractionation

Microcolumn abstracts

Ion trap mass spectrometry as detector for capillary electrochromatography of peptides: Possibilities and limitations

Microcolumn abstracts

Microcolumn abstracts