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Asthma is an increasingly common disease characterized by allergic airway inflammation, hyperresponsiveness, mucus overproduction, and peribronchial fibrosis. The epithelial cytokines IL25 have been implicated in asthma pathogenesis because they promote Th2-type cytokine synthesis. The current study aimed to assess IL-25 expression and measure the serum level of this cytokine in asthmatic Iraqi patients. The study was conducted on sixty (60) asthmatic patients (32 female and28 male) and thirty as apparently healthy subjects (control) (16 female and 14 male) with ages ranging from 15-60 years recruited Alzahra’a Center for Asthma and Allergy in Baghdad during the period between November /2021 to March /2022. Written informed consent was obtained from all patients and a healthy control group. Moreover, the study design was approved by the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies/ University of Baghdad. All patients were diagnosed according to clinical examination by a chest physician and selected according to the global initiative of asthma criteria. The healthy control group are non-smoking subjects with no history of obstructive lung disease or atopy. The RNA was extracted from the whole blood of asthma patients and healthy control without Trizol subjects using the protocol in Easy Pure t® blood RNA Kit (ER-401). The acceptable purity of asthmatic patients and control healthy group RNA ranges between 1.84 and 1.99. IL-25 gene expression showed the Ct of asthma patient group (25.179) and control group (27.393), and the2-ΔΔCt of asthma patient group (288.81) and control group (58.48) and the fold of gene expression was statistically significantly (P≤0.01) the calculation of the gene expression it was significant in the patient group than control group4.938 times with ratio (4.938:1) . ELISA estimated IL-25concentration, the results were statistically significant (P≤0.001), the level of IL-25 in asthma patient group (29.30± 5.107317pg/ml) while the level of IL-23 in Control group (15.16±0.085248pg/ml). Keywords: IL-25, gene expression, Elisa, Asthma

Basima Q. Hasan ALSaadi

Mayada Hussain Ali

Association of serum level and gene expression of IL-25 in a sample of Iraqi asthmatic patients

伊拉克哮喘患者血清水平与IL-25基因表达的关联

El objetivo de este estudio fue evaluar el potencial funcional del fruto de Macha Macha (Vaccinium floribundum Kunth), a partir de diferentes formulaciones de una bebida isotónica con aceptación sensorial y calidad nutricional, evaluando la composición físico- química de la fórmula optimizada. La metodología de la investigación fue implementada en tres fases experimentales: en la primera fase, se elaboraron diferentes formulaciones de la bebida isotónica con 5% (T1), 10% (T2) y 15% (T3) de pulpa, más el testigo (T4); para la segunda fase, se evaluaron las características físico-químicas de las distintas formulaciones, y por último, las formulaciones se evaluaron mediante análisis sensorial de escala hedónica de 9 puntos con 60 panelistas tipo afectivos para determinar la fórmula optimizada. Los resultados demuestran que la concentración de pulpa aplicando tratamiento térmico a la bebida isotónica no afecta las características físico-químicas de las formulaciones, los cuales se encuentran dentro de los parámetros normales reportados para las bebidas isotónicas. Mientras que, la evaluación sensorial mostró que la bebida isotónica de mayor aceptabilidad y mejor formulada y parecida al control, fue el tratamiento tres con (10 % de pulpa). Se concluye que, la elaboración de bebidas isotónicas con pulpa de frutos tiene cada vez más mayores preferencias por los consumidores, libres de aditivos y que contengan compuestos bioactivos que puedan mejorar el rendimiento de los atletas. Palabras clave: formulación, bebida isotónica, análisis sensorial, pulpa, potencial funcional.

Luis Solorzano

Jhunior Marcia

Roberto Chuquilín

Franklin Areche

Alejandro Herrera

Jenny Ruiz

Evaluación de la Pulpa de Macha Macha (Vaccinium floribundum Kunth), en el Desarrollo de una Bebida Isotónica

马查浆果（Vaccinium floribundum Kunth）果浆在开发等渗饮料中的评估

Microorganisms with a high pathogenic potential for humans and animals can spread through water. This represents a severe threat to public health, particularly in low- and middle-income countries where sanitation conditions are often limited. Due to their constant presence in the intestine and feces of both people and animals, total coliforms, thermotolerant coliforms, and intestinal Enterococcus have historically been employed as indicators of fecal contamination to evaluate water quality for human use and consumption. Under no circumstances should water sources for human use and consumption have bacteria indicating fecal contamination. The objective of this study was to evaluate the microbiological quality of surface water from six sub-basins located in the upper basin of the Cholulteca River in Honduras and determine the Escherichia coli phylogroups isolated in these samples. Our results show high rates of fecal contamination, suggesting that surface waters in the upper Choluteca River basin are unsafe for human consumption. Phylogroups B1 and D were the most frequent among 29 E. coli isolates, while phylogroups C and F were the least frequent. Measures must be taken to raise awareness about sanitation and good practices for managing household waste and those generated by agro-industrial and livestock activities in the area. Surface water in the Choluteca River basin must be adequately treated before consumption because it may impact people's health. In addition, we encourage prompt decision-making by the health authorities. In conclusion, this study assessed the microbiological quality of water in the upper Choluteca River basin. Our findings show high rates of fecal contamination and the predominance of E. coli strains from phylogroups associated with fecal contamination, suggesting that these waters are unsafe for human consumption. Keywords: fecal contamination; E. coli; phylogroups; surface water; Choluteca River basin; Honduras

Victoria Maldonado

Keylin Mendoza

Luis Rivera

Tania Peña

Marcio Chirinos-Escobar

Lourdes Enríquez

Gustavo Fontecha

Bryan Ortiz

High microbiological contamination in surface waters in the upper basin of the Choluteca River in Honduras

洪都拉斯乔卢特卡河上游流域地表水高度微生物污染

Understanding dental composite resins' physical and mechanical properties is vital for advancing dental materials science, enhancing durability, and improving patient care. The present study aims to quantitatively investigate the impact of compressive forces and thermocycling aging on the strength of mono incremental dental composite resins, focusing on three materials: 3M Filtek One Bulk Fill, Opus Bulk Fill APS A2 from FGM, and Tetric N-Ceram Bulk Fill VIA from Ivoclar Vivadent. Using a mixed quasi-experimental approach, 84 samples were divided into a control group and an experimental group, comprising 14 samples of each material. The samples underwent a thermocycling process involving temperature cycles between 5°C, 37°C, and 55°C, totaling 10,800 cycles, simulating one year of aging. Subsequently, compressive strength tests were conducted using a universal press to identify the fracture point of the material and calculate its strength. Our results reveal differences in the material before and after thermocycling; however, these differences were not statistically significant concerning strength. Thus, it is concluded that one year of thermocycling aging does not induce substantial changes in the resin that would compromise its strength. Future research should consider extended thermocycling tests spanning multiple years to pinpoint the potential onset of issues affecting material strength durability. Keywords: compressive strength, dental composite resins, thermocycling, aging, mono incremental.

Cristian Roberto Sigcho Romero

Henry Fabricio Mejía Mosquera

Sandra Marcela Quisiguiña Guevara

Yudy Jacqueline Alvarado Aguayo

Influence of compressive forces and aging through thermocycling on the strength of mono incremental dental composite resins

压缩力和热循环老化对单步增量牙科复合树脂强度影响的探讨

The current study was conducted between the beginning of October 2022 and the end of March 2023 to identify the prevalence of Cryptosporidium parivum infection among children under five years using microscopic and molecular methods. The study recorded 43(37.71%) infected children diagnosed using the Modified Zeihl Neelsen Stain and Gimsa stain. On the other hand, a molecular examination of stool samples using PCR showed that 25(21.92%) children out of 115 collected stool samples were infected with this parasite. DNA Sequencing was performed for specimens diagnosed with Cryptosporidium parasite, and the new isolates were registered for the first time in Nineveh governorate in NCBI under the accession number 757376. Keywords: Cryptosporidium disease, Genetic map.

Hibba Alobide

Firas Alkhashab

Hiyam Altaee

Genetic map of the isolated Cryptosporidium parasite from children with diarrheal in the city of Mosul

摩苏尔市腹泻儿童分离隐孢子虫的遗传图谱

La medicina es una amalgama de arte y ciencia, que parte del subjetivismo de una entrevista (anamnesis) y pasa al objetivismo del examen físico, la analítica, la imagen y se soporta con técnicas de monitoreo de los sistemas que cada vez son más sofisticados y que se alimentan actualmente de algoritmos clínicos derivados de machine learning e inteligencia artificial. Pese a los avances descritos, si el primer punto que es la extracción correcta de datos en la entrevista clínica es deficiente, todo lo subsiguiente puede desvanecerse El motivo de relatar este caso clínico es para reflexionar, sobre como un dato extraído con intuición clínica, revelo un antecedente destacado, que formuló un diagnóstico impensado y que con la terapeútica adecuada cambió un rumbo trágico inminente en un paciente jóven, que pese a estar con un monitoreo avanzado y en ventilación mecánica no tenía diagnóstico y su pronóstico era fatal a corto plazo. Palabras claves: anamnesis, exploración física, analítica, diagnóstico por imagen, seguimiento, aprendizaje automático, inteligencia artificial

Jorge Luis Vélez-Páez Vélez-Páez

Jhonny Carranza

Manuel Luis Avellanas Chavala

La anamnesis, una herramienta diagnóstica poderosa en tiempos de medicina tecnológica e inteligencia artificial. Análisis y reflexión de un caso

病史：在科技与人工智能时代的强大诊断工具——案例分析与反思

Many chemicals and their harmful effects and diseases have been linked as causative agents of diseases. The research included knowledge of the effect of continuous exposure to environmental pollutants on the blood components of individuals living in Mosul (an urban area) and Al-Sheikhan district (a rural area). Eighty blood samples were collected from males only, non-smokers, alcohol abusers and those free of chronic diseases. Their ages ranged Between (21-55) years old; the samples were divided into two groups. The first group included (40) blood samples from Mosul, while the second group included (40) samples from individuals from the Al-Sheikhan district in the same age groups. The results showed a significant increase in Red Blood cell count (RBCS), Hemoglobin concentration (Hb) and Packed Blood Cell Volume (PCV) in the group of males living in the city of Mosul compared with the group of males living in the Al-Sheikhan district. In contrast, the results showed a significant increase in White Blood cell count (WBCS), Erythrocyte Sedimentation Rate (ESR) and Platelet Count (PLT) in a group of males living in Al-Sheikhan district compared with a group of males living in Mosul city. Keywords: Environmental Areas, Environmental Pollution, Blood Components, Rural, Urban.

Raneen Mohammed AL-Shuraifi

Zahra Izideen Yonis Dallalbashi

The Effect of Environmental Pollution on Blood Components of Individuals in Different Environmental Areas (Rural and Urban)

不同环境区域（农村和城市）环境污染对个体血液成分的影响

El raquis de la palma africana (Elaeis guineensis) es considerado uno de los subproductos obtenidos en las plantas extractoras de aceite, el mismo provoca contaminación ambiental, sin embargo, con los avances biotecnológicos se puede aprovechar este residuo, al utilizarlo como sustrato para el crecimiento de microorganismos y a su vez aumentar la concentración de proteínas en el mismo. Fueron formulados cuatro medios a partir del raquis previamente secado y triturado e inoculado Aspergillus niger a una concentración de 5000 y 50000 conidios/g de medio por un tiempo de 8 y 12 días. Mediante un diseño factorial 22 , se determinó que la concentración del inóculo y el tiempo de fermentación influyeron significativamente sobre el enriquecimiento proteico del raquis, resultando que en el cultivo donde se inoculó una concentración de 50000 conidios/g con una duración de 8 días se alcanzaron valores de 15,5 mg/ml de proteínas. Palabras claves: hongo filamentoso, fermentación microbiana, fermentación en estado sólido

Lisbeth Cristina Dumas

Danae Fernández

Incremento proteico del raquis de la Palma Africana (Elaeis guineensis) utilizando Aspergillus niger

利用黑曲霉提高非洲棕榈（Elaeis guineensis）茎部蛋白质含量

Research on bioprospecting bioactive secondary metabolites from marine bacteria has rapidly expanded in the past few decades. The complexity of the environment in which bacterial communities live involves complex interactions with other communities competing for resources for survival. Previously, the ability of Pseudovibrio denitrificans has been demonstrated to inhibit the growth and virulence of different patho-gens such as Vibrio parahaemolyticus. The antagonism of these bacteria has been studied, considering the latter's importance in producing farmed shrimp. The molecules produced by P. denitrificans may be fun-damental for its antibacterial effect. In the present work, we examine LC/MS profiles of the interaction under two different circumstances: direct and delayed antagonism. The results show marked differences in the chemical composition between both interactions and the tentative identification of antibacterial and antibiofilm compounds. The observed profile could be part of P. denitrificans chemical arsenal to halt V. parahaemolyticus growing. The presented data is relevant to designing future studies on bacterial antago-nism with relevant implications for the aquaculture industry. Keywords: vibriosis, probiotics, chemometrics, white shrimp.

Ricardo Palma

Bolivar Chalén

Noroska G.S. Mogollón

Jenny Rodríguez

Cristian Quiroz-Moreno

Initial analysis of profiles from Pseudovibrio denitrificans and Vibrio parahaemolyticus antagonism by LC-MS-based metabolomics

基于LC-MS代谢组学的假单胞菌和副溶血性弧菌拮抗作用初步分析

El objetivo del estudio es determinar el efecto inhibitorio de Atorvastatina cálcica sobre el crecimiento bacteriano in vitro, el cual serviría para evidenciar un efecto pleiotrópico de este compuesto. Se realizó mediante la técnica de difusión en papel, en las que se enfrentaron a 3 cepas ATCC: Pseudomonas aerugi-nosa, Escherichia coli y Staphylococcus aureus, con 6 repeticiones para cada una. El sistema de ensayo estuvo formado por concentraciones de Atorvastatina cálcica de 250 μg y 500 μg; también se utilizó control positivo: Aztreonam 30 μg, Trimetoprima/sulfametoxazol 25 μg y Cefoxitina 30 μg y control negativo etanol. Los datos fueron procesados en Stata v17. Los resultados mostraron halos de inhibición de 6 mm en las concentraciones de 250 μg y de 500 μg de Atorvastatina para todas las muestras experimentales. In vitro Atorvastatina a concentraciones de 250 μg y 500 μg, no presentó efecto inhibidor frente a las cepas bacte-rianas estudiadas. Palabras clave: Antibacterianos, Atorvastatina, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, pleiotrópico

José Yareta Yareta

Lucely Aycachi-Centeno

Vasti Hurtado-Pérez

Stefani Laureano-Condori

Alberto Salazar-Granara

Pool Marcos Carbajal

Potencial efecto pleiotrópico de la Atorvastatina: inhibidor del crecimiento bacteriano, estudio in vitro

阿托伐他汀的多效性潜力：细菌生长抑制剂，体外研究

ISSN	1390-9347, 1390-9355
h-index	14

涉及主题	生物医学化学遗传学内科学生物化学哲学病理艺术人文学科地质学生态学物理
出版信息	出版商: Clinical Biotec, Universidad Catolica del Oriente (UCO)，出版周期: ，期刊类型: journal，开源期刊: 否
基本数据	创刊年份: 2016，原创研究文献占比: 0.00%，自引率:N.A.， Gold OA占比: 0.00%
平均审稿周期	网友分享经验：8 Weeks

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