Journal of Biological Research (Italy)

Diabetes Mellitus (DM) and hypertension are two common diseases that impact a significant section of the population . Pro-inflammatory mediators such as Interferon Gamma (IFN-γ) and C-Reactive Protein (CRP) levels are proposed to be linked to these diseases. The present study was aimed to explore the link between IFN- γ, CRP and lipid profile levels In type 2 diabetes mellitus (T2DM) connected to hypertension. The current study included 78 patients who had T2DM and 40 samples were collected from healthy people, both aged from 35 to 60 years. The total number of T2DM patients group were divided into two groups: 41 patients with Hypertension (HP) and 37 patients without HP, and according to their Body Mass Index (BMI). In addition, the relationship between gender and these diseases was also investigated. The results showed that the levels of Total Cholesterol (TC), Triglycerides (TG), Low-Density Lipoproteins (LDL), and Very Low-Density Lipoproteins (VLDL) were significantly increased in T2DM patients in both women and men. The results also showed a significant increase in the levels of TC, TG, LDL and VLDL in overweighted T2DM patients with HP. However, the levels of High-Density Lipoprotein (HDL) were significantly decreased in all T2DM patients. Moreover, the levels of CRP were significantly increased in T2DM female and male patients. However, the levels of IFN-γ significantly decreased in T2DM female and male patients. Furthermore, body fat percentage and waist/hip ratio significantly increased and were associated with Fasting Blood Sugar (FBS), TC, and CRP in T2DM patients. The findings confirm that lipid profile and pro-inflammatory parameters, such as CRP and IFN-γ, could have a significant impact on T2DM burden that is connected to the presence of HP. Therefore, regulating of lipid profile and pro-inflammatory parameters (CRP and IFN-γ) could protect against development and progression of T2DM accompanied by HP.

Raed Madhi

Nidhal A. Hashim

Zainab A.J.R. Al-Ali

Noor Tahir Tahir

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale

Estimation of lipid profile and some inflammatory biomarkers in patients with diabetes mellitus type 2 linked to hypertension

2型糖尿病合并高血压患者血脂谱及部分炎症标志物的评估

Industrial advancements, while driving economic growth, have simultaneously intensified environmental pollution levels, posing significant health risks to populations residing near industrial hubs. The release of a complex mixture of pollutants, including heavy metals, particulate matter, and volatile organic compounds, has been linked to a range of adverse health outcomes, including respiratory diseases, cardiovascular complications, and cancer. The Badoush Cement Factor in Mosul city, Nineveh, Iraq, a major industrial facility, exemplifies this challenge, emitting substantial quantities of air pollutants capable of long-range transport. This study, therefore, investigated the potential gene-environment interactions focusing on TBXA2R and GSTP1 genes and contributing to respiratory health disparities among 64 employees of the Badoush Cement Factory compared to 10 control subjects. Specifically, the study employed the tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) reaction technique to analyze the role of the TBXA2R gene, known for its involvement in bronchoconstriction and susceptibility to asthma, and the GSTP1 gene, encoding a crucial antioxidant enzyme protecting lung tissue from oxidative stress. By examining these genetic markers within the context of occupational exposure at the Badoush Cement Factory, this research aimed to elucidate the intricate interplay between genetic predisposition and environmental factors in determining respiratory health outcomes. The results indicated that there was no direct correlation between exposure to pollutants and genetic variation in the TBXA2R gene. However, prolonged exposure to pollutants may increase the risk of developing respiratory diseases. No significant association was found between pollutants emitted from the cement plant and genetic variation in the GSTP1 gene.

Dema Mohammed Sabah

Mohammad Ibrahim Khalil

Genetic variation in &lt;i&gt;GSTP1&lt;/i&gt; and &lt;i&gt;TBXA2R&lt;/i&gt; genes: influence of Badoush cement factory pollutant

&lt;i&gt;GSTP1&lt;/i&gt; 和 &lt;i&gt;TBXA2R&lt;/i&gt; 基因的遗传变异：Badoush水泥厂污染物的影响

Hemolysis is a common pre-analytical error that can affect the accuracy of biochemical assay results. The aim of the study is to investigate the impact of hemolysis on the measurement of nine emergency biochemical parameters: glucose, creatinine, calcium, potassium, sodium, Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT),total bilirubin, and direct bilirubin. The study included blood samples collected from various units of the maternity hospital, including high-risk pregnancies follow-up, postpartum care, gynecology, neonatology, and intensive care. The study employs a Hemolysis Index (HI) to monitor and assess sample quality, determining their suitability for the analysis of the nine aforementioned biochemical parameters. The results reveal that hemolysis can significantly affect the accuracy of tests results for the studied parameters, particularly for potassium (increased 4-12 times) and AST (increased 1.47-48.55 times). Three of the studied substances showed a significant, negative, and moderate correlation with HI after the osmotic shock was induced, including AST (r = -0.501, p = 0.006), ALT (r = -0.516, p = 0.004), and sodium (r = -0.598, p = 0.001). Conversely, no significant association was found for the other parameters, which are: glucose (r = 0.079, p = 0.639); creatinine (r = 0.140, p = 0.402); direct bilirubin (r = 0.292, p = 0.075); total bilirubin (r = 0.272, p = 0.114); calcium (r = 0.215, p = 0.196); and potassium (r = -0.188, p = 0.258). Our findings indicate that HI may not be helpful for calculating a predicted value for samples with HI ranging between 87.30 and 295.9. Therefore, it is crucial to establish a threshold for the degree of hemolysis beyond which releasing the result would be considered potentially harmful to the patient especially new-borns and premature infants. In conclusion, the appropriate use of HI in clinical laboratories can enhance patient care quality by minimizing the risk of misdiagnosis leading to inappropriate treatment, particularly when requesting a second sample is not feasible.

Sofiane Bouazza

Amine Bouarfa

Ali Khalfa

Slimane Brikhou

Abbassia Demmouche

The hemolysis index as a tool for monitoring mild hemolysis in biochemical assays at the emergency laboratory

溶血指数作为急诊实验室生化检测中轻度溶血监测工具

The recent acknowledgement of depleted uranium (DU) munitions utilization in the Ukrainian conflict has sparked renewed apprehensions regarding the safety of DU, its toxicological profile, and the health ramifications of exposure. Historical data from conflicts like the Gulf War, Bosnia, and Kosovo have recorded an upsurge in neoplastic ailments among soldiers in close proximity to DU deployment. Nevertheless, establishing a direct causal connection between DU exposure and the development of neoplastic diseases remains elusive, as indicated by meta-analyses and studies on animal models. We posit that the absence of a conclusive causal correlation between DU exposure and neoplastic diseases may be ascribed to the constraints of current study models, which fail to encapsulate the intricate interactions between DU and the human immune system, pathophysiology, particularly in the context of chronic, low-level exposure. Nowadays evidences suggests that DU exposure contributes to a cumulative immunotoxic effect, culminating in a compromised immune surveillance system and an escalated risk of neoplastic diseases over time. To investigate this hypothesis, we advocate for the advancement of pioneering research models, such as human ex-vivo body-on-a-chip systems, which can more accurately replicate the human physiological response to DU exposure and cancer pathophysiology. These models should encompass the examination of immune system modifications along with the potential for DU to interact with diverse organs and tissues, thereby furnishing a more comprehensive understanding of the enduring health impacts of DU.

Stefano Burgio

Valerio Gennaro

Olga Maria Manna

Domiziana Picone

Adelaide Carista

Francesco Cappello

Implication of depleted uranium in human carcinogenesis with a glance to implementation of novel and reliable experimental models

贫铀在人类致癌中的意义及对新型可靠实验模型实施的展望

This work aims to study whether there is a relationship between titer values of anti-SARS-CoV-2 IgG and changes in proteolytic processes. To confirm this hypothesis, we analyzed the content and activity of Matrix Metalloproteinases (MMPs) as well as the concentration and composition of circulating peptide pools in the plasma of blood donors divided into groups on the basis of anti-SARS-CoV-2 IgG titers. The results of gelatin zymography showed the presence of active MMP-2 in donors’ plasma who recovered from COVID-19. In contrast, collagenases and their complexes were detected in the plasma of donors with no anti-SARS-CoV-2 IgG, while their activity was undetectable in some groups of COVID-19 convalescent individuals. The content of MMPs also differed among the donors with different titers of anti-SARS-CoV-2 IgG. Plasma peptide content was identical among the donors' groups, but there were more peptide fractions in plasma peptide pools of COVID-19 convalescent individuals; furthermore, they all were characterized by the presence of peptides with molecular weights less than 920 Da and greater than 1530 Da. We hypothesized a link between proteolytic alterations and peptide fraction composition. Our data need further validation to confirm the relationship between the titer values of anti-SARS-CoV-2 IgG and the severity of the proteolytic imbalance.

Tetiana Halenova

Antonina Rachkovska

Daryna Krenytska

Oleksandra Kostiuk

Vitalii Karbovskyy

Tetiana Vovk

Nataliia Raksha

Olexii Savchuk

Liudmyla Ostapchenko

Abnormal proteolytic activity profile in plasma of blood donors according to anti-SARS-CoV-2 IgG titer

血清抗SARS-CoV-2 IgG滴度相关的献血者血浆异常蛋白水解活性谱

短名	J. Biol. Res.
Journal Impact	0.60
国际分区	BIOLOGY(Q4)

ISSN	1826-8838, 2284-0230
h-index	12

涉及主题	生物医学化学遗传学生物化学内科学地理历史物理地质学病理有机化学考古
出版信息	出版商: Page Press Publications，出版周期: ，期刊类型: journal，开源期刊: 是
基本数据	创刊年份: 2011，原创研究文献占比: 81.25%，自引率:20.00%， Gold OA占比: 98.39%
期刊费用	$详情
平均审稿周期	网友分享经验：10 Weeks

Journal of Biological Research (Italy)

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Estimation of lipid profile and some inflammatory biomarkers in patients with diabetes mellitus type 2 linked to hypertension

Genetic variation in <i>GSTP1</i> and <i>TBXA2R</i> genes: influence of Badoush cement factory pollutant

The hemolysis index as a tool for monitoring mild hemolysis in biochemical assays at the emergency laboratory

Implication of depleted uranium in human carcinogenesis with a glance to implementation of novel and reliable experimental models

Abnormal proteolytic activity profile in plasma of blood donors according to anti-SARS-CoV-2 IgG titer

Estimation of lipid profile and some inflammatory biomarkers in patients with diabetes mellitus type 2 linked to hypertension

Implication of depleted uranium in human carcinogenesis with a glance to implementation of novel and reliable experimental models

Genetic variation in <i>GSTP1</i> and <i>TBXA2R</i> genes: influence of Badoush cement factory pollutant

The hemolysis index as a tool for monitoring mild hemolysis in biochemical assays at the emergency laboratory

Abnormal proteolytic activity profile in plasma of blood donors according to anti-SARS-CoV-2 IgG titer