BIOCHIP JOURNAL 发表以下学科生物芯片技术所有领域的原创研究和评论，包括蛋白质芯片、DNA 芯片、细胞芯片、芯片实验室、生物 MEMS、生物传感器、微/纳米力学、微流体、高通量筛选技术、医学科学、基因组学、蛋白质组学、生物信息学、医学诊断学、环境监测和微/纳米技术。该杂志致力于快速同行评审，以确保发表最高质量的原创研究和及时的新闻和评论文章。

The Biochip Journal publishes original research and reviews in all areas of biochip technology, including protein chips, DNA chips, cell chips, lab-on-a-chip, bio-MEMS, biosensors, micro/nano mechanics, microfluidics, high-throughput screening technologies, medical sciences, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring, and micro/nano technologies. The journal is committed to rapid peer review to ensure the publication of high-quality original research and timely news and review articles.

《Biochip Journal》发表生物芯片技术各领域的原创研究和评论，包括蛋白质芯片、DNA芯片、细胞芯片、芯片实验室、生物MEMS、生物传感器、微/纳米力学、微流体、高通量筛选技术、医学科学、基因组学、蛋白质组学、生物信息学、医学诊断学、环境监测以及微/纳米技术。该杂志致力于快速同行评审，以确保发表最高质量的原创研究和及时的新闻与评论文章。

Biochip Journal

Abstract Although surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique with unbeaten sensitivity, the capabilities of SERS have been not fully utilized in screening applications because throughput of spectrum detection by conventional Raman instruments has been restricted due to their single-point measurement manners. Hence, this paper presents a development of a high throughput Raman screening system that employs a fiber-optic switch and a Raman probe array. In the system, a 785 nm excitation light is directed into the 1 × 8 broadband optical switching device and selectively switched to one of 8 output ports connected to the corresponding Raman probe array to deliver the light to samples under each probe. This optical switching driven probing in sequence allows us to rapidly detect Raman scattering of the multiple ( n = 8) samples in array within a short time (~ 28 s) with decent sensitivity (10 –7 M). The Raman spectroscopy of the system is validated by comparing the features of Raman spectra obtained from vitamin C tablets with those from a commercial Raman microscope and the detection sensitivity is measured with SERS substrates with different concentrations. Then, feasibility of high throughput screening is tested with a SERS chip array.

Seung-Jin Lee

Kwan Hee Lee

Young-Wan Choi

Jun Ki Kim

Woo June Choi

BioChip Journal

Optical-Switch Raman Spectroscopy for High Throughput Screening

用于高通量筛选的光开关拉曼光谱技术

Since coronavirus disease 2019 (COVID-19) pandemic rapidly spread worldwide, there is an urgent demand for accurate and suitable nucleic acid detection technology. Although the conventional threshold-based algorithms have been used for processing images of droplet digital polymerase chain reaction (ddPCR), there are still challenges from noise and irregular size of droplets. Here, we present a combined method of the mask region convolutional neural network (Mask R-CNN)-based image detection algorithm and Gaussian mixture model (GMM)-based thresholding algorithm. This novel approach significantly reduces false detection rate and achieves highly accurate prediction model in a ddPCR image processing. We demonstrated that how deep learning improved the overall performance in a ddPCR image processing. Therefore, our study could be a promising method in nucleic acid detection technology.

Young Suh Lee

Ji Wook Choi

Taewook Kang

Bong Geun Chung

Deep Learning-Assisted Droplet Digital PCR for Quantitative Detection of Human Coronavirus

Keying Li

Jeongtae Kim

Yujin Jeong

Moo-Seung Lee

Chiwan Koo

On-Site Food Toxin Detection System Using a Gb3-Immobilized Microchip and a Portable Fluorescence Sensing System

现场食品毒素检测系统：利用Gb3固定化微芯片和便携式荧光传感系统

Particulate matter (PM10)-induced respiratory illnesses are difficult to investigate in trans-well culture systems. Microphysiological systems offer the capacity to mimic these phenomena to analyze any possible hazards that PM10 exposure poses to respiratory system of Humans. This study proposes an on-chip healthy human lung distal airway model that efficiently reconstitutes in vivo-like environmental conditions in a microfluidic device. The lung-on-chip model comprises a TEER sensor chip and portable microscope for continuous monitoring. To determine the efficacy of our model, we assessed the response to exposure to three PM environmental conditions (mild, average, and severe) and analyzed the relevant in vivo physiological and toxicological data using the airway model. Our results revealed significant increases in the levels of the IL-13, IL-6, and MUC5AC pathological biomarkers, which indicate increased incidences of on-chip asthma and chronic obstructive pulmonary disease conditions. Overall, we deduced that this model will facilitate the identification of potential therapeutics and the prevention of chronic life-threatening toxicities and pandemics such as COVID-19. The proposed system provides basic data for producing an improved in organ-on-chip technology.

Faiza Jabbar

Young-Su Kim

Sang Ho Lee

Biological Influence of Pulmonary Disease Conditions Induced by Particulate Matter on Microfluidic Lung Chips

颗粒物诱导肺部疾病条件对微流控肺芯片的生物学影响

Hyunjik Oh

Minjin Kang

Eunji Bae

Yonghun Jung

Jinhui Cho

Joscillyn Poirier

Jong Sung Kim

John P. Frampton

Nakwon Choi

Seok Chung

Fabrication of Hydrogel Microchannels Using Aqueous Two-Phase Printing for 3D Blood Brain Barrier

水相双相打印制备水凝胶微通道用于三维血脑屏障

Hyogu Han

Jae Hoon Jeung

Yuna Cho

Jun Ki Ahn

A Label-Free, One-Step Isothermal Assay for Sensitive Detection of Telomerase Activity Using Light-Up Aptamer

无标记、一步等温检测端粒酶活性的高灵敏度荧光适配体法

Abstract Ligand-gated ion channels are involved in numerous pathophysiological processes and are, therefore, important targets for drug discovery. We describe a new microfluidic lab-on-a-chip device that allows the recording of fast ligand-gated ion channel kinetics in Xenopus laevis oocytes by non-invasive electrical impedance spectroscopy (EIS). The device, designated as Aachen Fast Ion Channel Sensor (AFIS) system, consists of two chambers separated by an orifice for capturing an oocyte by applying suction to the lower chamber. For channel recordings, annular electrodes are placed above and below the orifice. As a proof of concept, we recorded the EIS responses of the X. laevis oocyte-expressed human P2X2 receptor (hP2X2R), which functions as a non-desensitizing adenosine triphosphate (ATP)-gated cation-selective channel. The impedance data were fitted with an appropriate equivalent electrical circuit model. After ATP application, both EIS and TEVC recordings showed similar fast responses, which reflected the rapid solution exchange. Concentration–response curves determined by EIS or TEVC were virtually similar, indicating that agonist potency can be reliably determined by EIS. The oocytes survived the EIS measurements intact and can be reused for further electrophysiological or other purposes. The AFIS system can be used with the noticeable electrical leakage between the oocyte and the orifice. This feature significantly reduces the amount of preparation time required to create a high-resistance seal. Therefore, the system offers the possibility to easy perform high-throughput drug screening, especially when multiple measurement sites are placed on one chip and a multiplexer is connected for simultaneous EIS measurements.

Yuan Cao

Linhan Cheng

Günther Schmalzing

Ralf Hausmann

Uwe Schnakenberg

Recording Fast Ion Channel Kinetics Using Non-invasive Electrical Impedance Spectroscopy

使用无创电阻抗谱记录快速离子通道动力学

Hwanju Jeong

Hakchun Kim

Jin-Sil Bae

Gayoung Lee

Seungyeon Han

Yuna Han

Erica E. Jung

Kwon-Yul Ryu

Yun Jung Heo

Inhee Choi

Transdermal Drug Delivery System Using Light and Moisture Dual Responsive Hybrid Microneedles

光与湿度双响应混合微针的透皮药物递送系统

Abstract In the practical application of organ-on-a-chip, mass production technology for flexible porous membranes is an essential element for mimicking the basement membrane of the body. Porous PDMS membrane is a promising material due to its high optical transparency, flexibility, and biocompatibility. However, the fabrication process is complex and costly. Even with soft lithography, a relatively straightforward method, there is a risk that the negative resist pillars used as molds peeling off from the substrate in mass production. In this study, we propose a novel mass production method for fabricating porous PDMS membranes using high-strength nickel (Ni) micropillars as molds by combining photolithography and electroforming technologies. The unibody structure of Ni micropillars ensures high reliability and provides a semi-permanent mold without degradation or detachment. We successfully fabricated two types of Ni micropillars and subsequently formed their corresponding porous PDMS membranes ( D (diameter) = 8 μm, P (pitch) = 30 μm, and D = 10 μm, P = 20 μm). The porous PDMS membrane showed non-inferiority to the control group in terms of viability when cultured with human vascular endothelial cells. Furthermore, we showed that the porous PDMS membrane can be used to evaluate the vascular permeability of nanoparticles.

Taiki Otomo

Hyunsoo Noh

Tatsuya Matsubara

Deok-Ho Kim

Masashi Ikeuchi

Kazuhiro Yoshida

Joon-wan Kim

Fabrication of Biomimetic Cell Culture Membranes Using Robust and Reusable Nickel Micropillar Molds

使用坚固且可重复使用的镍微柱模具制备仿生细胞培养膜

Abstract Lipid nanoparticles have become a promising agent for biocompatible, effective, stable, and clinically approved delivery system that protect the nucleic acid from degradation while allowing cellular uptake and release of nucleic acid. Herein, we report an advanced microfluidic device and system for synthesis of uniform shape and size of mRNA encapsulated LNPs. The device consists of five repeated donut ring structures with two inlets and one outlet microfluidic channels which are assigned for organic phase solution, aqueous phase solution, and LNP recovery, respectively. It also has a minimum microfluidic channel width of 100 μm and can produce LNPs with approximately diameter from 70 to 190 nm. These LNPs can be produced less than 2 min after loading of reagents in the instrument. To verify the practical applicability of the as-developed instrument, mRNA expressing enhanced green fluorescence protein (EGFP) is encapsulated in LNPs. The gene expression of mRNA encapsulated LNPs confirmed the mechanical and chemical stability of the LNPs and its capability of gene delivery. Based on the results, the microfluidic device and system can provide a simple, fast, reliable, easy-to-use, cost-effective, and reproducible way to be applicable in LNP-based gene therapies and vaccines for treating various diseases.

Daekyeong Jung

Soojin Jang

Donggeun Park

Nam Ho Bae

Chung Soo Han

Seunghwa Ryu

Eun-Kyung Lim

Kyoung G. Lee

Automated Microfluidic Systems Facilitating the Scalable and Reliable Production of Lipid Nanoparticles for Gene Delivery

自动化微流体系统促进脂质纳米颗粒的可扩展和可靠生产用于基因递送

Abbreviation	Biochip J.
Journal Impact	5.49
Quartiles(Global)	NANOSCIENCE & NANOTECHNOLOGY(Q2)

ISSN	1976-0280, 2092-7843
h-index	33

Publication Information	Publisher: Springer-Verlag GmbH and Co. KG，Publishing cycle: Quarterly，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 2008，Proportion of original research papers: 70.00%，Self Citation Rate:25.50%， Gold OA Rate: 7.35%
Average review cycle	网友分享经验：较慢,6-12周
Average recruitment ratio	网友分享经验：容易

Biochip Journal

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