African Journal of Microbiology Research

Three medicinal plants, Aloe vera, Mentha arvensis and Ocimum sanctum were explored for endophytic actinomycetes diversity, plant growth promoting and antimicrobial activity.Endophytic actinomycetes were most commonly recovered from roots (70% of all isolates) followed by stems (17.5%) and leaves (12.5%), respectively.Single genus Streptomyces ranked first (60% of all isolates) followed by Micromonospora (25%), Actinopolyspora (7.5%), and Saccharopolyspora (7.5%).The highest numbers of endophytic actinomycetes were isolated from Ocimum sanctum (45%).Out of 22 isolates tested, 12 showed the ability to solubilize phosphate in the range of 5.4-16.5 mg/100 ml, while 16 isolates produced indole-3 acetic acid (IAA) ranging between 8.3-38.8µg/ml.Nine isolates produced the amount of hydroxamate-type of siderophore ranging between 5.9-64.9µg/ml and only four isolates were able to produce catechol-type of siderophore in the range of 11.2-23.1 µg/ml.Of the nine, interestingly, eight endophytic actinomycetes (88.9%) showed a significant antagonistic activity against one or more phytopathogenic fungi indicating their possible role as plant biocontrol agents.An extended infection of root tissues of Ocimum sanctum by Saccharopolyspora O-9 was observed using transmission electron microscope (TEM).

Gangwar Madhurama

Dogra Sonam

Phutela Gupta Urmil

Nath Kharwar Ravindra

Diversity and biopotential of endophytic actinomycetes from three medicinal plants in India

印度三种药用植物内生放线菌的多样性和生物潜力

High global prevalence of multidrug-resistant bacteria due to antibiotics misuse has prompted the need for novel antibacterial compounds to replace the failing antibiotics. This study investigated some natural habitats in Abuja, Nigeria, for antibiotics-producing bacteria. Thirty-six soil samples from termite mounds, river banks and rhizospheres of Anacardium occidentale L. (cashew tree), Gmelina arborea Roxb. ex Sm. (beechwood), Ageratum conyzoides L. (goat weed) including Cymbopogon citratus (DC) Stapf. (lemon grass) were cultured on nutrient media. Twelve potential antibiotic-producing isolates were identified by crowded plates method and characterized using Bergey&rsquo;s manual. The antimicrobial activities of the filtrates from the isolates against some pathogenic strains namely Streptococcus pneumonia, Salmonella typhi, Escherichia coli (ATCC 25922), Proteus mirabilis, Staphylococcus aureus (ATCC 25923), Candida albicans, Bacillus subtilis and Pseudomonas aeruginosa (ATCC 27853) were determined by agar-well diffusion method. Only 5 eventually inhibited at least 1 test microorganism; 4 showed activities against both Gram-positive and Gram-negative bacteria (broad spectrum) and 2 among the 4 also inhibited Candida albicans, while the remaining 1 inhibited only 1 Gram-positive bacterium (narrow spectrum). The 5 potent antibiotics-producers were Bacillus spp.&nbsp; In conclusion, some natural habitats in the FCT are important sources of antibiotic-producing bacteria.&nbsp; Their antimicrobial lead compounds could be extracted and developed locally for pharmaceutical applications.&nbsp;&nbsp; &nbsp; Key words:&nbsp; Antibiotics-producing bacteria, Bacillus species, natural habitats.

Olumuyiwa Balogun Olasinbo

Chukwudi Ugoh Sylvanus

Olawale Oladosu Peters

Antibiotic-producing bacteria isolated from some natural habitats in the Federal Capital Territory (FCT), Nigeria

尼日利亚联邦首都特区(FCT)某些自然生境中分离的产抗生素细菌

Ivorian forest has been experiencing degradation for several decades despite reforestation efforts. The main cause of the failure of reforestation policies is an important mortality at the time of planting (transplanting stress). To remedy this problem, mycorrhization technology based on the beneficial effects of arbuscular mycorrhizal fungi (AMF) could provide a sustainable solution. The objective of this study was to evaluate the effects of AMF inocula (local and commercial inoculum) on the juvenile growth of a C&ocirc;te d&#39;Ivoire forest species (Guibourtia ehie). Vegetative growth parameters and mineral nutrition (N, P, K, and Ca) were evaluated. After 150 days of cultivation, the plants treated with the local inoculum had the highest mycorrhization frequencies (75%) and intensities (21.23%). Also for growth parameters (height, number of leaves, leaf area, and crown diameter) and for nitrogen and potassium contents, the plants treated with the local polyspecific inoculum had the highest values compared to the plants treated with the commercial inoculum and the control plants. Mycorrhization improved mineral nutrition as well as vegetative growth of G. ehie seedlings. The integration of mycorrhizal inoculation from local strains in reforestation policies could be a sustainable solution for the recolonization of degraded forests by endangered species. &nbsp; Key words: Guibourtia ehie, inoculum, mycorrhization mineral nutrition, vegetative growth, Ivory Coast.

Bi Rosin Voko Don-Rodrigue

Clément Kouassi Kouassi

Athanase Kouassi Kra

Konaté Ibrahim

Impact of mycorrhization on transplanting stress and the juvenile growth of an Ivorian forest species Guibourtia ehie (Fabaceae, (A. Chev.) J. Leonard)

菌根化对科特迪瓦森林树种Guibourtia ehie（豆科，A. Chev.）J. Leonard）移栽胁迫和幼苗生长的影响

The study investigated microbial population dynamics in soils of Greater Port Harcourt Area, Nigeria. The study involved the quantification of petroleum pollutants and the enumeration and characterization of culturable fungi and bacteria. Gas Chromatograph FID method was used to quantify total petroleum hydrocarbons (TPH) in the soil while morphological and biochemical tests were used to characterize the microorganisms. The highest TPH was 9,759.37&plusmn;883.36 ppm which exceeded the Department of Petroleum Resources&rsquo; (DPRs&rsquo;) intervention value of 5,000 ppm. The percentage of hydrocarbon utilizing fungi (% HUF) ranged between 1.93&plusmn;0.57 and 66.55&plusmn;5.60 which were observed in sites A1 (Aluu) and CA (Agricultural control), respectively. The percentage of hydrocarbon utilizing bacteria (% HUB) ranged between 0.12&plusmn;0.01 and 7.89&plusmn;1.25 which was observed in sites A3 (Emuoha) and CU (Urban control), respectively. The most prevalent bacterial species includes Bacillus subtilis, Micrococcus lylae, Staphylococcus aureus, Bacillus cereus and Alcaligen faecalis. The most prevalent HUB isolate was Bacillus subtilis. The most prevalent fungal isolates were Aspergillus niger, Aspergillus flavus and Candida torulopsis. A. niger was the most prevalent among the HUF isolates which was identified in 12 sites. It was concluded that the study sites harboured TPH degrading microorganisms that are appropriate for bioremediation of TPH polluted sites. &nbsp; Key words: Total petroleum hydrocarbons, soil pollution, biodiversity, fungi, bacteria.

M. P. Wanjala

L. Odokuma

I. Etela

R. Ramkat

B. A. Odogwu

Characterization of culturable microbial community in oil contaminated soils in Greater Port Harcourt Area, Nigeria

尼日利亚哈科特港大区受石油污染土壤中可培养微生物群落的特征研究

The aim of the present study was to understand the seasonal occurrence and diversity of species of meropenem-resistant bacteria in the Gumuncheon river receiving effluents from a pharmaceutical industry in Seoul, Korea.Water samples were collected from the Gumuncheon river in Kyoung-gi province during winter (January), spring (April), summer (August), and fall (November) of 2018. Water samples were plated in triplicate on tryptic soy agar plates containing 16 mg/L meropenem. Meropenem-resistant bacteria were isolated and genetically identified using 16S rRNA analysis. The predominant bacterial genera identified were Elizabethkingia, Pseudomonas, Chryseobacterium and Stenotrophomonas. Among these; Pseudomonas species Pseudomonas chengduensis and Pseudomonas taiwanesis showed resistance against 15 antibiotics. To prevent the occurrence and spread of meropenem-resistant bacteria in rivers, it is necessary to implement methods that can simultaneously kill multi-drug resistant bacteria and remove antibioticsfrom pharmaceutical industry effluent discharge. Further, to stop the spread of meropenem-resistant bacteria in environment, effluent discharge water should be stringently assessed for their risk of being an environmental hazard. &nbsp; Key words: Carbapenem, Meropenem, Multi-drug resistant, Elizabethkingia, Pseudomonas, Chryseobacterium, Stenotrophomonas.

Ho Hwang Sung

Jin Kim Young

Profiling of meropenem-resistant bacteria in a river receiving wastewater effluent from a pharmaceutical industrial unit

河流中来自制药工业单位废水排放的耐美罗培南细菌的特征分析

Antwi Apenteng John

Amanor Mfoafo Kwadwo

Odoi Hayford

Orman Emmanuel

Cecil Dodoo Cornelius

Exploring the antimicrobial modulatory potential of the sap from oil palm tree

探索油棕树汁液的抗菌调节潜力

The objective of this study was to describe the resistance profile of enterobacterals isolated from urine samples at the laboratory of Donka National Hospital. Urine samples were collected from both outpatients and hospitalized patients. Cultures were performed using standard techniques, strains were identified using the API 20E kit, and antibiotic susceptibility testing was carried out using the ATB™ UR EU (08) kit. The results were interpreted according to the recommendations of the Antibiogram Committee of the French Society of Microbiology (CASFM v1 2023). Out of a total of 520 urine samples analyzed, 111 were positive for enterobacteral strains. Among them, 75 (67.57%) were of community origin. Escherichia coli was the most represented species (n=61, 55%), followed by Klebsiella pneumoniae (n=24, 22%). The resistance of E. coli strains to third generation cephalosporins (TGC) varied from 5.41% (n=6) to 25.23% (n=28) in the community and from 13 to 38% in the hospital. The profile for carbapenems was categorized as "susceptible to high dosage (SHP)," accounting for 16.22% (n=18). This study provided insight into the resistance profile to antibiotics used in urinary tract infections. The increasing resistance to carbapenems poses a threat to the management of strains producing extended-spectrum beta-lactamases (ESBL). It would be important to strengthen resistance surveillance in this context. Key words: Enterobacteral, urinary tract infection, resistance, community, hospital, Guinea.

KABA Djiba

NIABALY Ousmane

DIALLO Issiaga

Sory Nana SYLLA Ibrahima

G. HOUNMENO Castro

Jean-Jacques Olivier KADIO Kadio

Badara TOURE Aly

SALL Boubacar

BONGONO Emile

KABA Lancei

Ousmane BARRY Alpha

DIABY Maladho

BALAMOU Thomas

KOIVOGUI Pascal

Yaramon KOLIE Ouo-Ouo

Sadjo DIALLO Mariama

CISSE Mohamed

Resistance profile of urine isolate enterobacteral strains at Donka University teaching hospital in Conakry, Guinea

几内亚科纳克里唐卡大学教学医院尿液分离肠杆菌菌株的耐药性特征

The prokaryotic microorganism Mycoplasma gallisepticum (MG) belongs to the phylum Firmicutes, the order Mycoplasmatales, the family Mycoplasmataceae, and the genus Mycoplasma. MG parasitizes the respiratory tract of various poultry species, causing a range of symptoms from indistinct features to tracheitis and air sacculitis. Common signs include respiratory tract infection indicators such as coughing, asthma, a runny nose and rales. Both domestic and international reports underscore MG's transmission and infection capacity, signifying its global importance as a source of poultry infection. Further research is imperative to grasp its impact on both farmed and wild animals. This article comprehensively reviews current research progress on MG, encompassing its biological characteristics, infection traits, pathogenic mechanisms, epidemiology, detection methods, treatment, and control measures. It aims to serve as a reference for comprehensive prevention and control strategies against MG in the future. Key words: Mycoplasma galliscepticum, infection characteristics, epidemiological characteristics, detection methods, treatment, vaccine.

Yang Ruizhi

Lin Xi

Song Huiqi

Zhou Hongmiao

Li Shuang

Li Xuejiao

Hao Bin

Li Lianrui

Mycoplasma galliscepticum: An overview

支原体鸡毒霉形体：概述

Nyamaifofe Dieudonné

Mbugua Amos

Mbogo Kevin

Characterization of novel potential probiotic strains of lactic acid bacteria from rat faeces

大鼠粪便中新型潜在益生菌乳酸菌的特性研究

The use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates.This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil.The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil.The bacteria were authenticated and evaluated regarding to their symbiotic capacity.The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box-(PCR).Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures.For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted.The majority of the obtained isolates showed rapid growth and acidified the culture medium.Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates.The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut.

涉及主题	生物化学遗传学生物化学地质学古生物学医学细菌微生物学有机化学基因植物食品科学生态学病理物理免疫学内科学园艺
出版信息	出版商: Academic Journals，出版周期: Monthly，期刊类型: journal，开源期刊: 否
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African Journal of Microbiology Research

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Diversity and biopotential of endophytic actinomycetes from three medicinal plants in India

Antibiotic-producing bacteria isolated from some natural habitats in the Federal Capital Territory (FCT), Nigeria

Impact of mycorrhization on transplanting stress and the juvenile growth of an Ivorian forest species Guibourtia ehie (Fabaceae, (A. Chev.) J. Leonard)

Characterization of culturable microbial community in oil contaminated soils in Greater Port Harcourt Area, Nigeria

Profiling of meropenem-resistant bacteria in a river receiving wastewater effluent from a pharmaceutical industrial unit

Exploring the antimicrobial modulatory potential of the sap from oil palm tree

Resistance profile of urine isolate enterobacteral strains at Donka University teaching hospital in Conakry, Guinea

Mycoplasma galliscepticum: An overview

Characterization of novel potential probiotic strains of lactic acid bacteria from rat faeces

African Journal of Microbiology Research