荧光方法和应用侧重于荧光光谱、成像、显微镜、荧光探针、标记和（纳米）材料的新发展。它将以方法和高级（生物）应用为特色，并接受原始研究文章、评论和技术说明。

The journal "Methods and Applications in Fluorescence" focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labeling, and (nano) materials. It will feature methods and advanced (bio) applications, and will accept original research articles, reviews, and technical notes.

《Methods and Applications in Fluorescence》期刊专注于荧光光谱、成像、显微镜、荧光探针、标记和（纳米）材料等领域的新发展。期刊将重点介绍方法和高级（生物）应用，并接受原创研究文章、评论和技术说明。

Methods and Applications in Fluorescence

The current culture-based bacterial detection technique is time-consuming and requires an extended sample preparation methodology. We propose the potential of surface-enhanced Raman spectroscopy (SERS) and surface plasmon-enhanced auto-fluorescence spectroscopy (SPEAS) for the label-free identification and quantification of bacterial pathogens at low concentrations collecting its unique auto-fluorescence and Raman signatures utilising highly anisotropic three-dimensional nanostructures of silver nano dendrites (Ag-NDs). The SERS data facilitates qualitative bacterial identification using the spectral features coming from the bacterial cell wall compound, and the SPEAS data was utilised to gain unique auto-fluorescence spectra present on the bacterial cell wall with enhanced quantification. The enhancement of Raman and auto-fluorescence signals of Ag-NDs were first evaluated using rhodamine 6g (R6G) as a probe molecule that exhibits a significant enhancement of 106 and limit of detection (LOD) of 10-12 M for SERS and 15 fold intensity enhancement and LOD of 10-15 M for SPEAS measurements. Further, the SERS and SPEAS measurements of bacterial pathogens, such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), using the Ag-NDs were recorded, and the results exhibit high auto-fluorescence and Raman signal intensity for both the samples up to 100 cfu/ml for both modalities. The significant photon count and distinct emission range in SPEAS measurements of bacteria enables accurate quantification. Therefore, the comprehensive investigation of plasmonic enhancement of Ag-NDs for SPEAS and SERS techniques provides complementary information about molecules to enable accurate and quick identification and quantification of pathogens.

Anjika Panghal

Sathi Das

Pramila Thapa

Meenakshi Meenakshi

Anand Kumar

Priya Nagpal

Satish Kumar Dubey

Vivekanandan Perumal

Dalip Singh Mehta

Surface plasmon enhanced auto-fluorescence and Raman spectroscopy for low-level detection of biological pathogens

表面等离子体增强的自发荧光和拉曼光谱用于低水平生物病原体检测

David J S Birch

Marcia Levitus

Yves Mely

MAF Journal and Conference Build Together

MAF期刊与会议共同建设

Abstract We studied absorption and fluorescence as well as room temperature phosphorescence (RTP) of 4-methylumbelliferone (4MU) in poly (vinyl alcohol) (PVA) films. We focused our study on the long-wavelength basic form of 4MU with absorption centered at 375 nm. The strong fluorescence with a quantum yield of above 70% appears at ~ 430 nm. The fluorescence anisotropy of 4MU-doped PVA film is high, reaching a value of about 0.3. The emission with gated detection shows a broad phosphorescence spectrum with a peak at ~ 510 nm and a residual delayed fluorescence at 430 nm. The excitation spectra for fluorescence and phosphorescence roughly follows 4MU absorption. The phosphorescence lifetime of 4MU is remarkably long, almost 3 s. 4MU excitation and emission phosphorescence anisotropies are low, very close to zero.

Bong Lee

Emma Alexander

Danh Pham

Mariusz Gagoś

Arkadiusz Matwijczuk

Zygmunt Gryczynski

Ignacy Gryczynski

Spectral properties of 4-methylumbelliferone in PVA films; long-lived room temperature phosphorescence

聚乙烯醇薄膜中4-甲基伞形酮的光谱性质；长寿命室温磷光

Abstract The escalating prevalence of hospital-acquired infections poses a critical challenge for healthcare systems worldwide. Effective management requires rapid identification of pathogens and their antibiotic resistance profiles. In this study, we utilized the photoconvertible mEos4b protein, which transitions from green to red fluorescence upon blue light exposure, to distinguish live from dead bacteria. The mEos4b gene was cloned into a prokaryotic vector and expressed in Escherichia coli BL21. The Minimum Inhibitory Concentration (MIC) of the transgenic bacteria was determined for five antibiotics, followed by a post-antibiotic effect assessment over a two-hour exposure period. The optimal photoconversion time for mEos4b was established as 90 seconds, and confocal microscopy was used to visualize live (green) and dead (red) cells post-exposure. The mEos4b-TR system proved highly specific, accurately distinguishing live and dead bacteria without producing false positives, even in control groups, which is a common issue in commercial live-dead kits. By relying on cellular metabolic activity rather than dyes, this system minimizes nonspecific interactions and contamination, making it more reliable than traditional methods prone to false readings. These results highlight the potential of the mEos4b-TR system as a superior alternative for rapid, precise bacterial viability assessments, particularly in determining antibiotic susceptibility. This preliminary study demonstrates the system’s differentiation of viable and non-viable cells, suggesting its potential application in future studies involving novel antibacterial agents to refine antibiotic sensitivity testing.

Ilknur Yilmaz

Humeyra Demir

Aleyna Eslem Tureyen

Tulin Ozbek

Detection of Antimicrobial-Induced Survival/Dead Bacteria via mEos4b Photoconversion: A Preliminary Study

通过mEos4b光转化检测抗菌剂诱导的存活/死亡细菌：一项初步研究

Abstract Linker-Evolved-Group-Optimized-Lipophosphonoxins (LEGO-LPPO) are small synthetic modular peptidomimetics with promising antimicrobial activity. The LEGO-LPPO mechanism of antibacterial action has been determined to be the depolarization and disruption of bacterial membranes. Their modular nature is advantageous for fine tuning their biological properties. In order to optimize the structure of LEGO-LPPO even further, it is important to understand the interaction of LEGO-LPPO with bacterial membranes at the molecular level. In this work, we present the synthesis of five LEGO-LPPO (designated as 1_naph2-4-G to 5_naph2-4-G) molecules bearing fluorescent naphtylethyl moieties and their usage in the study of LEGO-LPPO behaviour in the membrane. Our goal was to characterize fluorescently labelled LEGO-LPPO under conditions that do not completely disrupt the membrane, mostly in the form of membrane-bound monomers.&amp;#xD;We observed the intramolecular interactions of hydrophobic modules of 1_naph2-4-G in the buffer by detecting dynamic naphthyl excimers and their disappearance after 1_naph2-4-G bind into the membranes. In the membrane, the molecule 1_naph2-4-G slightly affects the membrane fluidity of DOPG membranes above the phase transition. The naphthyl fluorophore itself has fast and almost unrestricted rotation around ethylene linking groups (rinf=0.010), which indicates a considerable chaotropic effect of the hydrophobic modules of 1_naph2-4-G at the given depth of the membrane.&amp;#xD;1_naph2-4-G proved to be a useful model for observing the interaction of LEGO-LPPO antibiotics with the phospholipid bilayer enabling us to decipher its effects on membrane state and dynamics; its binding and penetration into the membrane, its structure and the particular depth that it occupies.&amp;#xD;

Tereza Dolejšová

Petra Liskova

Nitjawan Sahatsapan

Viktor Mojr

Radek Pohl

Hana Brzobohatá

Milica Dugić

Tomáš Křížek

Lukasz Cwiklik

Gabriela Mikušová

Dominik Rejman

Radovan Fiser

Naphthylated LEGO-lipophopsphonoxin antibiotics used as a fluorescent tool for the observation of target membrane perturbations preceding its disruption

萘基化LEGO-脂亲磷肟抗生素作为荧光工具用于观察目标膜扰动在其破坏之前的现象

Abbreviation	Methods Appl. Fluoresc.
Journal Impact	2.20
Quartiles(Global)	CHEMISTRY, PHYSICAL(Q3)

ISSN	2050-6120
h-index	34

Publication Information	Publisher: IOP Publishing Ltd.，Publishing cycle: ，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 2013，Proportion of original research papers: 91.80%，Self Citation Rate:4.20%， Gold OA Rate: 23.81%

Methods and Applications in Fluorescence

Journal Citation Format

A journal article with 1 author

A journal article with 2 authors

A journal article with 3 authors

A journal article with 5 or more authors

Books Citation Format

Thesis Citation Format

Web sites Citation Format

Patent Citation Format

Staying up late manually editing references? ivySCI automatically matches journals and helps you generate references with a single click.

Share Submission Experience

Surface plasmon enhanced auto-fluorescence and Raman spectroscopy for low-level detection of biological pathogens

MAF Journal and Conference Build Together

Spectral properties of 4-methylumbelliferone in PVA films; long-lived room temperature phosphorescence

Detection of Antimicrobial-Induced Survival/Dead Bacteria via mEos4b Photoconversion: A Preliminary Study

Naphthylated LEGO-lipophopsphonoxin antibiotics used as a fluorescent tool for the observation of target membrane perturbations preceding its disruption

Surface plasmon enhanced auto-fluorescence and Raman spectroscopy for low-level detection of biological pathogens

MAF Journal and Conference Build Together

Spectral properties of 4-methylumbelliferone in PVA films; long-lived room temperature phosphorescence

Detection of Antimicrobial-Induced Survival/Dead Bacteria via mEos4b Photoconversion: A Preliminary Study

Naphthylated LEGO-lipophopsphonoxin antibiotics used as a fluorescent tool for the observation of target membrane perturbations preceding its disruption