Prakticke Lekarnictvo

The qualitative and quantitative composition of fruit extracts of 23 forms of 7 barberry species (Berberis amurensis, B. heteropoda, B. iliensis, B. integerrima, B. oblonga, B. sibirica and B. thunbergii) collected on the territory of Kazakhstan and Uzbekistan has been studied. The extracts were obtained by adding 70% ethanol to the crushed fruits in a ratio of 1:5 (raw material-reagent) and subsequent separation with organic solvents, namely n-hexane, chloroform and ethyl acetate. Chromatographic analysis was conducted on Agilent 7890 A GC gas chromatography with Agilent 5975 inert MSD quadrupole mass spectrometer as a detector. The number of components found in the fruits of the 7 barberry species studied varies from 10 to 61. The identified components belong to various classes of chemical compounds as fatty acids, fatty acid esters, alkanes, alkenes, alkadienes, aromatic hydrocarbons, aldehydes, fatty alcohols, phenols, triterpenoids, ketones, and silicones. Squalene, phylloquinone, pyridine-3-carboxymidamide and propanamide were also found in all the samples studied, except B. oblonga. Also, in the all investigated Berberis species free monosaccharides as glucose, galactose, xylose and arabinose were found. The obtained information on the component composition of barberry can be used for the production of new medicines of a wide spectrum of action.

N.V. Romadanova

L.N. Karasholakova

K.A. Eshbakova

S.V. Kushnarenko

International Journal of Biology and Chemistry

Study of the component composition of seven Berberis speсies

七种小檗属植物成分组成的研

All over the world, there is an active search for genes that are responsible for the formation of predisposition to allergic diseases, which is associated both with the undoubted relevance of studying risk factors for the development of allergies, and with the emergence of new opportunities for genetic research. In addition, anthropogenic impact can cause allergic reactions and modifications in the functions of antioxidant defense cells and the immune system. Due to the fact that in the cohort of the population of Almaty and the Almaty region (going forward we will say Almaty to include the region also), respiratory diseases with an allergic component, such as bronchial asthma, bronchitis, allergic rhinitis, are common, we consider it is appropriate to conduct an epidemiological study of candidate genes for allergic diseases – cytokines and their receptors (IL4, IL4RA , IL12B, IL13, TNFA, CCL5), adrenoreceptor (ADRB2), transcription factors involved in T-lymphocyte differentiation (STAT6, GATA3, TBX21), major histocompatibility complex (HLA-DRB1, HLA-DQB1). The aim of our study is to conduct genome-wide genotyping in people with allergic diseases and to search for marker genes that affect the risk of developing allergies as well as to identify genes that are involved in the pathogenesis of allergic diseases. We performed genome-wide microarray genotyping of 25355 SNPs on the iScan platform of 103 samples with allergic diseases and 108 control DNA samples using the kit (Infinium® ImmunoArray-24 v2.0 BeadChip Kit). The results obtained, using the GWAS analysis, which takes into account numerous polymorphisms, showed the involvement of some genes in the development of allergic diseases (rs20541-asthma, allergic rhinitis, rs841718-atopic dermatitis, rs3212227-immunodeficiency).

N.K. Altynova

O.K. Khamdiyeva

Zh.A. Zhaniyazov

A.A. Garshin

G.S. Baratzhanova

A.S. Amirgalieva

K. Yergali

D.N. Artygaliyeva

D.Zh. Talgatbekova

S.S. Tokmurzina

M.S. Abdullayeva

G.S. Zhunussova

L.B. Djansugurova

A genome-wide association study for different types allergic diseases

不同类型过敏性疾病的基因组关联研究

The article presents the results of isolation of the bacterial pathogen Pseudomonas parafulva from a diseased Siberian sturgeon (Acipenser baerii) grown in a recirculating aquaculture system. The studied diseased individuals of the Siberian sturgeon (Acipenser baerii) were characterized by reduced activity, did not consume compound feed, on the body of some individuals there were hemorrhages at the bases of the fins, as well as deep penetrating ulcers. Internal organs (liver, spleen and kidneys) and washes from open ulcers were used to isolate the bacterium. As a result of studies of biological materials, the dominant bacterium was used for further identification. The bacterium is characterized as a gram-negative, motile oxidase-positive rod, in the Voges-Proskauer and methyl red test negative, in the O/F test it is characterized as oxidative. As a result of molecular genetic analysis of the 16S rRNA gene sequence of the isolated bacterium, 99% identity with other strains of P. parafulva was determined. Subsequently, the isolated strain was named AB004, and the 16S rRNA sequence was registered in the National Center for Biotechnology Information (NCBI) database under registration number OK634400. According to the results of the physiological characteristics of AB004, the optimal indicators of cultivation in the Luria-Bertani (LB) medium are: NaCl concentration - 1%, pH value - 7.0, temperature - 37°C.

S.S. Bakiyev

I.T. Smekenov

N.B. Baltakhozha

A. Kauysbekov

A.K. Bissenbaev

Isolation, identification and physiological growth characteristics of Pseudomonas parafulva from diseased Acipenser baerii

患病施氏鲟中铜绿假单胞菌的分离、鉴定及生理生长特性

The experience of studying the process of alkylation of toluene with butene in the presence of a catalyst based on natural zeolite shows the high fundamental importance of toluene to butene ratio in the reaction zone with a uniform concentration distribution of butene over the entire surface of the catalyst. Such process conditions differ in activity, selectivity, as well as the duration of the solid catalystoperation. The synthesis of a catalyst based on natural zeolite was effectively carried out by the absorption method. The range of values of the main parameters and general properties of the catalyst synthesis that ensure the effective conduct of the alkylation reaction is determined. The study of acid centers was carried out by the method of direct registration of OH groups of catalysts based on natural zeolite and IR spectroscopy. Subsequently, the acidic properties of decationized and heat-treated zeolite, and catalysts based on it were investigated. X-ray structural analysis was performed in the laboratory of physical and chemical research methods to determine the mineral and chemical composition of Koskuduk amorphous rock clays. The 5 phases were identified based on the results of X-ray analysis of the studied kaolinite clay: α-quartz- SiO2, calcite СаСО3, mica R1R2-3 [AISi3O10](OH, F)2, R1 = К, Na; R2 = Al, Mg, Fe, Li; mixed-layer mineral and chlorite impurities.

H. Arbag

S. Satayeva

Zh. Yerzhanova

F.Zh. Akhmetova

B. Khamzina

A. Kairgaziyeva

A. Beimbetova

Research of zeolite catalysts for the process of alkylation of aromatic hydrocarbons

芳香烃烷基化过程中沸石催化剂的研究

This study aims to determining the volatile oil components of naturally distributed Viscum album L. belonging to the Santalaceae family. The study was carried out in villages of Eğirdir province of Isparta in Turkey. The isolation of volatile components was performed from of the leaves of the mistletoe collected from five different tree species. Qualitative analysis of volatile components were carried out by the Head Void-Solid Phase Micro Extraction (HS-SPME) technique combined with gas chromatography/mass spectrometry (GC-MS). The identification of the constituents was carried out by comparing the retention index (RI) and mass spectral data (MS) to those reported in the published literature. As a result of the Head Void-Solid Phase Micro Extraction (HS-SPME) technique combined with gas chromatography/mass spectrometry (GC-MS). 54 different components were determined and (E)-2-Hexenal, Benzaldehyde and Nonanal were found as the main components. It is known that there are limited number of studies on the determination of essential oil and volatile components of the Viscum genus. According to this study, use of mistletoe as a raw material source in different industrial areas have importance

E.H. Tigli Kaytanlioglu

A.G. Sarikaya

H. Fakir

Determination of volatile components of Viscum album L. distributed naturally in Eğirdir (Isparta) province, Turkey

土耳其埃伊尔迪尔（伊斯帕尔塔）省自然分布的槲寄生挥发性成分的测定

Leptoglossus occidentalis was first detected in Türkiye in 2009. The pest's damage to stone pine trees and its effects on seeds cause economic losses. In recent years, observations and predictions show that there is a significant threat not only to stone pine but also to other coniferous forests throughout country. In this study, pesticides were applied by trunk injection method in the experiment areas taken in stone pine stands of Bergama - Kozak region. In the systemic application carried out in 2020 and 2021, the Arborjet system was used and the insecticide with the active ingredient Azadirachtin (0.3 g/Lt) was used as an organic insecticide, as well as pesticide with the active ingredient Imidacloprid (200 g/Lt). In 2020, the highest seed filling rate was achieved after systemic Imidacloprid insecticide application with the Arborjet system. As a result of this application, the full seed rate reached 70.7% in Asagicuma, while this rate was 63.6% in the Karaveliler. The seed occupancy rate obtained from control trees in the Asagicuma was 37.96% and this rate reached 70.7% after systemic Imidacloprid application in the same field. Similarly, in the studies carried out in Asagicuma and Yukaribey experiment sites in 2021, only 35% of the seeds were filled in the cones taken from control trees, while the occupancy rate in the cones without insecticide application in the Yukaribey was determined to be 32.76%. In both trial sites, the highest seed fill rate was achieved after systemic Imidacloprid insecticide application with the Arborjet system. As a result of this application, the filled seed rate reached 67.89% in the Asagicuma and 69.86% in Yukaribey experiment sites.

O. Sarikaya

S. Parlak

Y. Yildiz

None T. Gencal

None I.M. Ozcankaya

None F. Selek

None O. Acici

None H. Can

Insecticide applications with trunk injection method for reducing the damage level of Leptoglossus occidentalis (Heidemann, 1910) in stone pine stands

树干注射法施用杀虫剂对减少石松林中西方松果小卷蛾（Leptoglossus occidentalis Heidemann, 1910）危害程度的研究

Alzheimer’s disease (AD) stands as the most prevalent form of dementia affecting elderly individuals and ranks as the sixth leading cause of death globally. The pathological hallmarks of AD involve the formation of beta-amyloid plaques and neurofibrillary tangles in the brains of affected individuals, contributing to progressive brain degradation. This study aimed to utilize molecular modeling methods as a theoretical approach to explore the inhibition of beta-amyloid plaques and neurofibrillary tangles. Beta-amyloid and tau receptors were employed to carry out molecular docking with the ligands, including curcumin, memantine, nicotine, and caffeine. The selected compounds demonstrated minimum binding affinity and interactions with the active sites of the receptors while docking studies were performed. Notably, molecular interactions of the receptor complexes with curcumin compounds exhibited prominence in number. Curcumin, known for its antioxidant, anti-inflammatory, and lipophilic properties, has shown promise in enhancing cognitive function in AD patients. The findings of this study highlight the potential for further research aimed at developing improved drugs based on curcumin for the treatment of AD.

S. Kashif

S. Feroze

A.A. Sethi

Targeting beta-amyloid plaques and neurofibrillary tangles: a proteomic approach towards Alzheimer’s disease therapy

针对β-淀粉样斑块和神经纤维缠结：一种蛋白质组学方法用于阿尔茨海默病治疗

Corrosion inhibitors find ubiquitous application across diverse sectors to formulate robust coatings and chemical compounds. Serving as pivotal additives in compositions, they play a vital role in crafting coatings for circulating water systems, water supply networks, oil refining, petrochemical industries, and power plants. Incorporation extends to fuels, oils, lubricants, and construction materials. In industries oxidation inhibitors is particularly high as chemical, electrochemical, petrochemical, and gas, as well as in water supply networks and circulating water systems. The utilization of inhibitors obviates the necessity for treating metal surfaces with protective enamels and mastics. Effectively preventing oxidation involves the introduction of chemical contributions into aggressive ecology, thereby neutralizing or retarding the oxidation processes. This article explores the anti-oxidation properties of inhibitors in compositions related to St-3 steel. The study includes factors such as the pH of the medium, the nature and concentration of phosphate in compositions simulating reservoir water under stationary and aerated conditions. Investigation methods as potentiometry and photocolorimetry used in research. Experimental data, quantitative parameters of the oxidation process, oxidation rate, and degree of protection, depth index, and stability of the film formed were determined. These indicators were assessed on the oxidation resistance scale relative to steel. Analysis of the data shows that out of 63 compositions, seven systems demonstrate the most significant protective effects, with five containing sodium orthophosphate and two containing sodium hydroorthophosphate. The effective, surrounding friendly and cost-effective steel oxidation inhibitors using materials from Kazakhstan will contribute to the development of industry,

None A.B. Niyazbekova

None T.A. Shakirov

None L.I. Baytlesova

None N.M. Zhunusbekova

A.I. Niyazbaeva

None G.M. Gubaidullina

Effect of phosphate nature on the inhibitory capability of phosphate compositions

磷酸盐性质对磷酸盐组合物抑制能力的影响

Cancer is now one of the major challenges in the medical world. An unhealthy lifestyle, and abuse or inappropriate use of drugs stimulate this disease. The drug resistance and imperfections of drugs used in cancer therapy have enforced the development of new drugs with therapeutic potential. Despite the introduction of synthetic drugs, the discovery of new drugs with natural origin has increasing attention in the treatment of diseases, recently. As natural compounds show a considerable diversity of chemical structures, they are liable for diverse mechanisms of action and interaction with target molecules. Heterocyclic natural compounds are a substantial part of natural compounds with excellent properties. These compounds possess various interaction modes with DNA. This review focus on the heterocyclic natural compounds with therapeutic potential and their binding mode with DNA.

M. Kamalabadi

Gh. Chehardoli

Aspects of DNA interaction with the natural heterocyclic compounds

天然杂环化合物与DNA相互作用的研究

The article presents studies of the effect of fecal suspension by introducing into the abdominal cavity at the rate of 0.5 ml of a 10% solution per 100 g of animal body weight. The morpho-functional state and morphometric analyzes of the small intestine were studied in normal conditions and with inflammation of the abdominal organs by introducing fecal suspension. The results showed that inflammation of the abdominal organs led to significant changes in the wall of the small intestine, multiple hemorrhages and fibrin clots were found. In the intestine, there is a change and violation of the structures of the small intestine, the total thickness of the mucous membrane increases. Violation of goblet cells, villus height and crypt depth occur. Analysis of the study showed that the walls of the small intestine, revealed structural changes, mainly in the mucosa, submucosa and muscle membranes. The mean value of the mucosal thickness in the control group was 526.17±17.11 micrometer; submucous layer – 47.21±1.63 micrometer. Phenomena of edema, inflammatory infiltration, and separation of muscle fibers were noted in both layers of the muscular membrane. The average layer of thickness of the muscular membrane was 145.67±6.92 µm. Changes in the mucous membrane of the small intestine, at one time reflected in the structural changes in the villi-crypt of the small intestine. A violation of microcirculation in the tissues of the small intestine after the inflammatory process was revealed, which leads to aggravation of dystrophic and necrobiotic lesions of the overall state of the small intestine and is combined with the severity of the clinical picture in experimental animals.

S.N. Abdreshov

M.A. Yessenova

A.N. Yeshmukhanbet

G.A. Demchenko

V.N. Gorchakov

G.K. Atanbaeva

None S.A. Mankibaeva

Morphofunctional and morphometric features of the small intestine in experimental rats with inflammation of the abdominal cavity

实验性腹腔炎症大鼠小肠的形态功能和形态计量学特征

短名	IJBCh
Journal Impact	0.20
国际分区	BIOLOGY(Q4)

涉及主题	生物化学有机化学生物化学医学物理遗传学材料科学工程类植物生态学地质学量子力学
出版信息	出版商: al-Farabi Kazakh National University，出版周期: ，期刊类型: journal，开源期刊: 否
基本数据	创刊年份: ，原创研究文献占比: 0.00%，自引率:0.00%， Gold OA占比: 100.00%

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