AIMS Microbiology

Vitamin C stands as an essential water-soluble vitamin, antioxidant and has been shown to enhance immunity. SARS-CoV-2 has been spreading rapidly across the worldwide, several cellular processes of innate and adaptive immunity are aided by vitamin C, which strengthens the immune system overall. Multiple lines of evidence in the literature associate vitamin C with antioxidant, anti-inflammatory, anticoagulant and immunomodulatory actions. Pneumonia and sepsis patients had poor ascorbic acid status and high oxidative stress, according to many studies. Pneumonia patients who get vitamin C may have less severe symptoms and a longer course of the illness if they do. To standardize plasma levels in sepsis patients, gram measurements of the vitamin must be administered intravenously (IV). This intervention has been shown in a few trials to reduce mortality. COVID-19 management in China and the United States has exhibited remarkable results when using a high percentage of intravenous vitamins C. It's acceptable to include vitamin C in the COVID-19 treatment protocol as a secondary measure based on the current active clinical studies looking at the impact of vitamin C on the management of COVID-19. Patients with hypovitaminosis C or severe respiratory illnesses, such as COVID-19, may benefit from taking vitamin C, due to its good safety profile, simplicity of use, and potential for rapid production scaling. The study's goal was to see whether high dosage intravenous vitamin C had any impact on individuals with severe COVID-19 (HDIVC). Finally we discuss recent research that has been published on the efficacy of vitamin C administration in the treatment of viral infection and life-threatening conditions. The purpose of this manuscript is to summarise existing research on the efficacy of vitamin C as a treatment for COVID-19 and to discuss possible explanations for why it may work in some individuals but not in others.

Umar Shahbaz

Nazira Fatima

Samra Basharat

Asma Bibi

Xiaobin Yu

Muhammad Iftikhar Hussain

Maryam Nasrullah

Role of vitamin C in preventing of COVID-19 infection, progression and severity

维生素C在预防COVID-19感染、进展和严重程度中的作用

&lt;p&gt;Little is known regarding the diversity patterns of Xylariaceae and Hypoxylaceae (Ascomycota) fungi taking part in the lignin decomposition of leaf litter from different tree species and under different climatic regions. The alpha and beta diversity of Xylariaceae and Hypoxylaceae fungi was investigated on bleached leaf litter from nine subtropical and cool temperate tree species in Japan. A total of 248 fungal isolates, obtained from 480 leaves from the nine tree species, were classified into 43 operational taxonomic units (OTUs) with a 97% similarity threshold and were assigned to nine genera of Xylariaceae and Hypoxylaceae. There was no overlap of fungal OTUs between subtropical and cool temperate trees. The mean number of fungal OTUs was generally higher in subtropical than cool temperate trees, whereas rarefaction curves depicting the numbers of OTU with respect to the number of leaves from which fungi were isolated were less steep in subtropical trees than in cool temperate trees, reflecting the dominance of major OTUs in the subtropical trees and indicating a higher species richness in cool temperate regions. Nonmetric multidimensional scaling showed general overlaps of fungal OTU compositions among tree species in the respective climatic regions, and one-way permutational multivariate analysis of variance indicated that the OTU composition was not significantly different between the tree species. These results suggest a wide host range and some geographic and climatic structures of distribution of these ligninolytic fungi.&lt;/p&gt;

Momoka Yoneda

Hiroki Ameno

Ayaka Nishimura

Kohei Tabuchi

Yuki Hatano

Takashi Osono

Diversity of ligninolytic ascomycete fungi associated with the bleached leaf litter in subtropical and temperate forests

亚热带和温带森林中白化落叶相关木质素降解子囊菌的多样性

&lt;p&gt;Sustainable alternatives are essential to improving agriculture production to meet the growing world's critical demands. Cyanobacteria and microalgae are considered renewable resources with a wide range of potential uses in the agricultural sector. We aimed to isolate cyanobacteria and microalgae from the mud of a carbon dioxide-rich sulfur pond and to investigate their plant growth-promoting (PGP) and soil bio-consolidating ability. Mud samples were subjected to DNA extraction and 16S rRNA gene sequencing to characterize the prokaryotic community. Phototrophic culturable microbiota was isolated and evaluated for different PGP properties. The most relevant isolates were combined in a consortium and used for &lt;italic&gt;in vitro&lt;/italic&gt; bioconsolidation activity. In a greenhouse experiment, the isolates were evaluated for their ability to promote salinity stress tolerance in sunflower plants. Metabarcoding results showed that most Amplicon Sequence Variants (ASV) were associated with Actinobacteriota (35%), Proteobacteria (19%), and Acidobacteriota (11%) at the phylum level and &lt;italic&gt;Unknown&lt;/italic&gt; (32%) and uncultured (31%) lineages at the genus level. The culture-dependent method yielded eight isolates associated with cyanobacteria and microalgae genera. The isolates obtained showed interesting PGP activities. Isolates C1, C2, and M1 were selected based on phosphate solubilization (85.6 µg PO&lt;sub&gt;4&lt;/sub&gt;&lt;sup&gt;3−&lt;/sup&gt; mL&lt;sup&gt;−1&lt;/sup&gt; on average), indoles (C1 and M1 0.54 µg mL&lt;sup&gt;−1&lt;/sup&gt; IAA equivalents on average), and ACC deaminase activity (C2 and M1 6.00 µmol α-KB mg proteins&lt;sup&gt;−1&lt;/sup&gt; h&lt;sup&gt;−1)&lt;/sup&gt;. The consortium efficiently consolidated sand particles in the presence of calcium carbonate by forming biomineralized aggregates. &lt;italic&gt;In planta&lt;/italic&gt; results showed positive effects of the consortium on &lt;italic&gt;Helianthus annuus L&lt;/italic&gt;., plant growth under normal conditions and salt stress. The positive effects on soil and plants indicated their effectiveness as bioconsolidants and biostimulant agents. Our findings highlight the interesting potential of cyanobacteria and microalgae applications in sustainable agriculture.&lt;/p&gt;

Beatrice Farda

Rihab Djebaili

Enrico Sabbi

Giancarlo Pagnani

Paola Cacchio

Marika Pellegrini

Isolation and characterization of cyanobacteria and microalgae from a sulfuric pond: Plant growth-promoting and soil bioconsolidation activities

硫酸池中蓝藻和微藻的分离与鉴定：促进植物生长和土壤生物固结活性

&lt;p&gt;Rat-bite fever (RBF) is a zoonotic infection and systemic febrile illness transmitted to humans by &lt;italic&gt;Rattus&lt;/italic&gt; spp. contacts following a scratch, bite, or touching excrement, such as urine, feces, and oral secretions. Infection with members of the genus &lt;italic&gt;Streptobacillus&lt;/italic&gt; is the most common cause of this infectious disease. In this review article, we updated the knowledge on the RBF caused by the genus &lt;italic&gt;Streptobacillus&lt;/italic&gt; based on the isolation and identification methods, virulence factors, clinical signs, differential diagnoses, antibiogram, treatment, geographical distribution, and epidemiology. Moreover, the present paper's comprehensive analysis of over 200 infection cases attributed to this genus, spanning from 1915 to 2023, sheds light on its epidemiology and provides valuable insights for the future.&lt;/p&gt;

Mehdi Fatahi-Bafghi

Rat-Bite Fever due to the genus &lt;i&gt;Streptobacillus&lt;/i&gt;

鼠咬热由<i>链杆菌属</i>引起

&lt;p&gt;The scab disease, caused by &lt;italic&gt;Elsinoe perseae&lt;/italic&gt;, poses a significant risk to avocado (&lt;italic&gt;Persea americana&lt;/italic&gt;) production in countries with warm and humid climates. Although the genome has been published, the precise virulence factors accountable for the pathogenicity of &lt;italic&gt;E. perseae&lt;/italic&gt; have not yet been determined. The current study employed an in silico approach to identify and functionally characterize the secretory proteins of &lt;italic&gt;E. perseae&lt;/italic&gt;. A total of 654 potential secretory proteins were identified, of which 190 were classified as carbohydrate-active enzymes (CAZymes), 49 as proteases, and 155 as potential effectors. A comparison to six other closely related species identified 40 species-specific putative effectors in &lt;italic&gt;E. perseae&lt;/italic&gt;, indicating their specific involvement in the pathogenicity of &lt;italic&gt;E. perseae&lt;/italic&gt; on avocado. The data presented in this study might be valuable for further research focused on understanding the molecular mechanisms that contribute to the pathogenicity of &lt;italic&gt;E. perseae&lt;/italic&gt; on avocado.&lt;/p&gt;

Biju Vadakkemukadiyil Chellappan

Comparative secretome analysis unveils species-specific virulence factors in &lt;i&gt;Elsinoe perseae&lt;/i&gt;, the causative agent of the scab disease of avocado (&lt;i&gt;Persea americana&lt;/i&gt;)

比较分泌蛋白组分析揭示了引起鳄梨疮痂病的病原菌&lt;i&gt;Elsinoe perseae&lt;/i&gt;的物种特异性毒力因子

&lt;p&gt;&lt;italic&gt;Staphylococcus lugdunensis&lt;/italic&gt; is a coagulase-negative species responsible for a multitude of infections. These infections often resemble those caused by the more pathogenic staphylococcal species, &lt;italic&gt;Staphylococcus aureus&lt;/italic&gt;, such as skin and soft tissue infections, prosthetic joint infections, and infective endocarditis. Despite a high mortality rate and infections that differ from other coagulase-negative species, little is known regarding &lt;italic&gt;S. lugdunensis&lt;/italic&gt; pathogenesis. The objective of this study is to identify the essential factors for biofilm formation in &lt;italic&gt;S. lugdunensis&lt;/italic&gt;. &lt;italic&gt;S. lugdunensis&lt;/italic&gt; was mutagenized through ethyl methanesulfonate (EMS) exposure, and the individual cells were separated using a cell sorter and examined for biofilm formation at 8 hr and 24 hr timepoints. Mutations that resulted in either increased or decreased biofilm formation were sequenced to identify the genes responsible for the respective phenotypes. A mutation within the &lt;italic&gt;S. lugdunensis&lt;/italic&gt; surface protein A (&lt;italic&gt;slsA&lt;/italic&gt;) gene was common among all of the low biofilm formers, thus suggesting that high expression of this protein is important in biofilm formation. However, other mutations common among the mutants with decreased biofilm formation were in the putative divalent cation transport gene, &lt;italic&gt;mgtE&lt;/italic&gt;. Conversely, a mutation in the gene that codes for the von Willebrand factor binding protein, &lt;italic&gt;vwbl&lt;/italic&gt;, was common among the mutants with increased biofilm formation. Following proteinase K treatment, a significant dispersal of the &lt;italic&gt;S&lt;/italic&gt;. &lt;italic&gt;lugdunensis&lt;/italic&gt; biofilm matrix occurred, thus confirming the presence of primarily protein-mediated biofilms; this is in agreement with previous &lt;italic&gt;S. lugdunensis&lt;/italic&gt; studies. Additionally, all low biofilm formers exhibited decreased protein levels (1.95–2.77 fold change) within the biofilm matrix, while no difference was observed with extracellular DNA (eDNA) or polysaccharides. This study presents a unique methodology to identify genes that affect biofilm formation and sheds light on &lt;italic&gt;S. lugdunensis&lt;/italic&gt; pathogenesis.&lt;/p&gt;

McKenna J. Cruikshank

Justine M. Pitzer

Kimia Ameri

Caleb V. Rother

Kathryn Cooper

Austin S. Nuxoll

Characterization of &lt;i&gt;Staphylococcus lugdunensis&lt;/i&gt; biofilms through ethyl methanesulfonate mutagenesis

通过甲基磺酸乙酯诱变分析<i>路邓葡萄球菌</i>生物膜的特性

短名	AIMSMICRO
Journal Impact	2.56
国际分区	MICROBIOLOGY(Q3)

ISSN	2471-1888
h-index	24

涉及主题	生物化学遗传学生物化学地质学古生物学细菌基因微生物学医学有机化学生态学食品科学内科学病理工程类物理植物
出版信息	出版商: AIMS Press，出版周期: ，期刊类型: journal，开源期刊: 是
基本数据	创刊年份: 2015，原创研究文献占比: 78.95%，自引率:0.00%， Gold OA占比: 96.30%
期刊费用	$500详情
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AIMS Microbiology

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Role of vitamin C in preventing of COVID-19 infection, progression and severity

Diversity of ligninolytic ascomycete fungi associated with the bleached leaf litter in subtropical and temperate forests

Isolation and characterization of cyanobacteria and microalgae from a sulfuric pond: Plant growth-promoting and soil bioconsolidation activities

Rat-Bite Fever due to the genus <i>Streptobacillus</i>

Comparative secretome analysis unveils species-specific virulence factors in <i>Elsinoe perseae</i>, the causative agent of the scab disease of avocado (<i>Persea americana</i>)

Diversity of ligninolytic ascomycete fungi associated with the bleached leaf litter in subtropical and temperate forests

Isolation and characterization of cyanobacteria and microalgae from a sulfuric pond: Plant growth-promoting and soil bioconsolidation activities

Rat-Bite Fever due to the genus <i>Streptobacillus</i>

Comparative secretome analysis unveils species-specific virulence factors in <i>Elsinoe perseae</i>, the causative agent of the scab disease of avocado (<i>Persea americana</i>)

Characterization of <i>Staphylococcus lugdunensis</i> biofilms through ethyl methanesulfonate mutagenesis