Annals of Blood

Mark T. Friedman

Victoria Costa

Behnam Rafiee

Timothy Hilbert

Mansab Jafri

Ding Wen Wu

A dose of platelets: getting it just right

血小板剂量：恰到好处

Aditi Khandelwal

Heather Vandermeulen

Bryan Tordon

Katerina Pavenski

Applying principles of patient blood management during COVID-19 pandemic: a literature review

在COVID-19大流行期间应用患者血液管理原则：文献综述

Luke Ka Lok Chan, Kenneth Wai Kin Tam, Chi Keung Wong, Jennifer Nga Sze Leung, Janette Siu Yin Kwok, Wai Chiu Tsoi, Cheuk Kwong Lee

Luke Ka Lok Chan

Kenneth Wai Kin Tam

Chi Keung Wong

Jennifer Nga Sze Leung

Janette Siu Yin Kwok

Wai Chiu Tsoi

Cheuk Kwong Lee

Provision of human leukocyte antigen (HLA) selected platelets: 10-year experience of a regional blood centre

人白细胞抗原（HLA）筛选血小板供应：地区血库10年经验

Abstract: There is a clinical need to determine the fetal blood group status for pregnant women who present with clinically significant red cell antibodies to optimize perinatal care. Non-invasive prenatal testing (NIPT) to assess the fetal blood group status is problematic for blood groups that arise from single nucleotide variants (SNVs) because the cell-free DNA from the maternal allele is present in greater abundance than the fetal allele, resulting in competition, especially in traditional real-time quantitative polymerase chain reaction (qPCR) platforms. This review discusses the power for digital (d)PCR to overcome this limitation. The dPCR is based on first diluting and compartmentalizing individual DNA molecules into individual PCR mixes, so that the competing (e.g., maternal) molecules are separated from the target (fetal) DNA molecule prior to amplification. In comparison to qPCR platforms, dPCR is amenable to the quantitation of a positive signal from an individual DNA molecule, providing the ability to calculate the fractional abundance of the fetal sequence of interest. Comparison studies, though small in number, show that dPCR is more precise and sensitive. Clinical feasibility studies to date show that dPCR for fetal Kell antigen, Rhc, RhE and Duffy antigens have high accuracy. The key limitation of the dPCR approach is that separate assays need to be established and validated for each blood group antigen. Despite that limitation, the power of dPCR for fetal blood group genotyping provides an added diagnostic tool in the management of women with red cell antibodies.

Catherine A. Hyland

Helen O’Brien

Eunike C. McGowan

Alexis J. Perros

Robert L. Flower

Genghis H. Lopez

Glenn J. Gardener

The power of digital PCR in fetal blood group genotyping: a review

数字PCR在胎儿血型基因分型中的应用：综述

Carine Prisco Arnoni

Emilia Sippert

Maria Rios

RH alleles: historical milestones, molecular advances, and clinical application

RH等位基因：历史里程碑、分子进展与临床应用

Zivile Jacike

Ema Rajackaite

Ramune Sepetiene

Ninette Robbins

Mohamed Ali

Predictive Model of variable laboratory findings calculation and monitoring during plasmapheresis procedures

预测性模型在血浆置换过程中实验室变量检测与监测中的应用

Sheinei Alan

Sebastian R. Mendez-Marti

Chad Zik

William B. Ershler

Advancing through challenges: current strategies in sickle cell disease management

克服挑战：镰状细胞病管理的当前策略

Wei Xuan Chue

Livia Anjaneth Lienardi

Titus Qi Wei Tan

Insyirah Abdul Kadir

Joyce Ching Mei Lam

Immune thrombocytopenia exacerbation post COVID-19 vaccination: a systematic review and meta-analysis

COVID-19疫苗接种后免疫性血小板减少症加重：一项系统综述和荟萃分析

Evandra Strazza Rodrigues

Flávia Leite Souza Santos

Tatiane Aparecida de Paula Vendrame

Talitha Baldin Cuter

Aline Romagnoli

Vanessa Mendes

Lilian Beatriz Moreira de Oliveira Chagas

Dimas Tadeu Covas

Lilian Castilho

Simone Kashima

Rh D-positive genotypes in Brazilian blood donors with D-negative phenotype

巴西D阴性血型献血者中的Rh D阳性基因型

Background: Mechanism of action of intravenous immunoglobulin (IVIG) is complex, and involves the immunoglobulin G (IgG) Fc fragment binding to Fc receptors on immune cells as activators and inhibitors. Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism that mainly depends on FcγRIIIA. However, the IVIG Fc fragment-binding activity to FcγRIIIA is unknown, and the IVIG-mediated ADCC remains to be clarified. This study attempts to explore the IVIG-mediated ADCC and provide correlative evidence. Methods: This study evaluated the IVIG-mediated ADCC using a time-resolved fluorescence (TRF) cell cytotoxicity assay in vitro, and Jurkat-NFAT-Luc-CD16 cells were used to detect the IVIG Fc fragment-binding activity to FcγRIIIA. Quantitative real-time polymerase chain reaction (PCR) analyses were conducted to detect mRNA synthesis when IVIG incubated with effector cells, and the final expression of FcγRIIIA on the cell membranes were detected by flow cytometry. Results: The results showed that IVIG had optimal binding activity to FcγRIIIA fitted with a four-parameter curve equation regression model with R2=0.99 and caused specific ADCC of about 40%. Meanwhile, IVIG upregulated the expression of FCGR3A by two-fold, and the FcγRIIIA level was markedly increased from 33.7% up to 66.7%. Conclusions: This study investigated the IVIG-mediated ADCC, including measurement of the IVIG Fc fragment-binding activity to FcγRIIIA and the regulation of FcγRIIIA expression by IVIG. The results indicate that ADCC that can kill tumor cells and IVIG can also promote FCGR3A synthesis, increasing FcγRIIIA expression on immune cells membranes. The findings provide further evidence for the IVIG-mediated ADCC and understanding of the biological activity of IVIG.

Liyuan Zhu

Yafei Zhao

Gang Ye

Yuwei Huang

Qing Liu

Peng Jiang

Changqing Li

Xi Du

Shengliang Ye

Li Ma

Intravenous immunoglobulin can induce antibody-dependent cell-mediated cytotoxicity and upregulate FCGR3A and FcγRIIIA expression in vitro

静脉注射免疫球蛋白可诱导抗体依赖性细胞介导的细胞毒作用，并在体外上调FCGR3A和FcγRIIIA表达

Publication Information	Publisher: AME Publishing Company，Publishing cycle: ，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 2019，Proportion of original research papers: ，Self Citation Rate:， Gold OA Rate:

Annals of Blood

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