为表彰这一非凡的科技时代，MARY ANN LIEBERT, INC. 的出版商最近宣布创建 THE CRISPR JOURNAL——这是一份国际性、多学科的同行评审期刊，发表关于 CRISPR 无数应用和基础技术的杰出研究。 2018 年，CRISPR 期刊将以灵活的开放获取选项在线和印刷出版，为开创性研究以及生动和挑衅性的评论、分析和辩论提供一个备受瞩目的场所。 CRISPR 期刊为 MARY ANN LIEBERT, INC. 的产品组合增加了一个令人兴奋和充满活力的组成部分，其中包括 GEN（基因工程和生物技术新闻）和 80 多个领先的同行评审期刊。

To celebrate this extraordinary era of technology, the publisher MARY ANN LIEBERT, INC. recently announced the creation of The CRISPR Journal. This is an international, multidisciplinary peer-reviewed journal dedicated to publishing outstanding research on the numerous applications and foundational technologies of CRISPR. Starting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a prominent venue for groundbreaking research as well as vibrant and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the portfolio of MARY ANN LIEBERT, INC., which includes GEN (Genetic Engineering and Biotechnology News) and over 80 leading peer-reviewed journals.

为了庆祝这一非凡的科技时代，MARY ANN LIEBERT, INC. 的出版商最近宣布创办《CRISPR Journal》。这是一份国际性、多学科的同行评审期刊，专注于发表关于CRISPR技术及其众多应用的杰出研究。自2018年起，《CRISPR Journal》将以灵活的开放获取选项在线和印刷出版，为开创性研究以及生动且富有挑战性的评论、分析和辩论提供一个备受瞩目的平台。《CRISPR Journal》为MARY ANN LIEBERT, INC.的产品组合增添了一个令人兴奋和充满活力的组成部分，涵盖了GEN（基因工程与生物技术新闻）及80多个领先的同行评审期刊。

CRISPR Journal

Treating human genetic conditions in vivo requires efficient delivery of the CRISPR gene editing machinery to the affected cells and organs. The gene editing field has seen clinical advances with ex vivo therapies and with in vivo delivery to the liver using lipid nanoparticle technology. Adeno-associated virus (AAV) serotypes have been discovered and engineered to deliver genetic material to nearly every organ in the body. However, the large size of most CRISPR-Cas systems limits packaging into the viral genome and reduces drug development flexibility and manufacturing efficiency. Here, we demonstrate efficient CRISPR gene editing using a miniature CRISPR-Cas12f system with expanded genome targeting packaged into AAV particles. We identified efficient guides for four therapeutic gene targets and encoded the guides and the Cas12f nuclease into a single AAV. We then demonstrate editing in multiple cell lines, patient fibroblasts, and primary hepatocytes. We then screened the cells for off-target editing, demonstrating the safety of the therapeutics. These results represent an important step in applying CRISPR editing to diverse genetic sequences and organs in the body.

Allison Sharrar

Zuriah Meacham

Johanna Staples-Ager

Luisa Arake de Tacca

David Rabuka

Trevor Collingwood

Michael Schelle

The CRISPR Journal

Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications

病毒递送紧凑型CRISPR-Cas12f用于基因编辑应用

The Pacific abalone is an important aquaculture shellfish and serves as an important model in basic biology study. However, the study of abalone is limited by lack of highly efficient and easy-to-use gene-editing tools. In this paper, we demonstrate efficient gene knockout in Pacific abalone using CRISPR-Cas9. We developed a highly effective microinjection method by nesting fertilized eggs in a low-concentration agarose gel. We identified the cilia developmental gene β-tubulin and light-sensitive transmembrane protein r-opsin as target genes and designed highly specific sgRNAs for modifying their genomic sequences. Sanger sequencing of the genomic regions of β-tubulin and r-opsin genes from injected larvae identified various genomic long-fragment deletions. In situ hybridization showed gene expression patterns of β-tubulin and r-opsin were significantly altered in the mosaic mutants. Knocking out β-tubulin in abalone embryos efficiently affected cilia development. Scanning electron microscopy and swimming behavior assay showed defecting cilia and decreased motility. Moreover, knocking out of r-opsin in abalone embryos effectively affected the expression and development of eyespots. Overall, this work developed an easy-to-use mosaic gene knockout protocol for abalone, which will allow researchers to utilize CRISPR-Cas9 approaches to study unexploited abalone biology and will lead to novel breeding methods for this aquaculture species.

Ruohui Li

Yue Xu

Fucun Wu

Zhangjie Peng

Jiulin Chan

Linlin Zhang

Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai)

易用的CRISPR-Cas9基因组编辑在培养的太平洋鲍鱼（Haliotis discus hannai）中

Tuberous sclerosis complex (TSC) is an autosomal dominant disorder caused by mutations in either the

Gongbo Guo

Morgan Moser

Lincoln Chifamba

Dominic Julian

Samantha Teierle

Prajwal Rajappa

Cecelia Miller

Mark E. Hester

CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2

CRISPR-Cas9介导的从结节性硬化综合症2型患者iPSCs中纠正TSC2致病性变异

The potato family includes a highly diverse cultivar repertoire and has a high potential for nutritional yield improvement and refinement but must in line with other crops be adapted to biotic and abiotic stresses, for example, accelerated by climate change and environmental demands. The combination of pluripotency, high ploidy, and relative ease of protoplast isolation, transformation, and regeneration together with clonal propagation through tubers makes potato highly suitable for precise genetic engineering. Most potato varieties are tetraploid having a very high prevalence of length polymorphisms and small nucleotide polymorphisms between alleles, often complicating CRISPR-Cas editing designs and strategies. CRISPR-Cas editing in potato can be divided into (i) characterization of target area and

Frida Meijer Carlsen

Ida Westberg

Ida Elisabeth Johansen

Erik Andreasson

Bent Larsen Petersen

Strategies and Protocols for Optimized Genome Editing in Potato

优化马铃薯基因编辑的策略与方案

Rodolphe Barrangou

CRISPR J

‘Tis the Season: CRISPR Products All Around

这是季节：CRISPR产品无处不在

The CRISPR-Cas9 system has been applied for clinical applications of gene therapy. Most CRISPR-based gene therapies are derived from

Siyuan Liu

Yingdi Zhao

Qiqin Mo

Yadong Sun

Hanhui Ma

Engineering CjCas9 for Efficient Base Editing and Prime Editing

高效碱基编辑和引物编辑的CjCas9工程化

Antibiotic resistance poses a global health crisis limiting the efficacy of available therapeutic agents. We explored CRISPR-Cas-based antimicrobials to combat multidrug resistance in methicillin-resistant

Aysegul Ates

Cihan Tastan

Safak Ermertcan

CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus

CRISPR-Cas9介导的耐甲氧西林金黄色葡萄球菌中多药耐药基因的靶向

Abbreviation	CRISPR. J.
Journal Impact	3.64
Quartiles(Global)	GENETICS & HEREDITY(Q2)

ISSN	2573-1599, 2573-1602
h-index	18

Publication Information	Publisher: Mary Ann Liebert Inc.，Publishing cycle: ，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 2019，Proportion of original research papers: 81.69%，Self Citation Rate:2.70%， Gold OA Rate: 19.46%

CRISPR Journal

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Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications

Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai)

CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2

Strategies and Protocols for Optimized Genome Editing in Potato

‘Tis the Season: CRISPR Products All Around

CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2

‘Tis the Season: CRISPR Products All Around

Strategies and Protocols for Optimized Genome Editing in Potato

Engineering CjCas9 for Efficient Base Editing and Prime Editing

CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus