RSC Chemical Biology

Overview of the joint profiling technologies of 5mC and 5hmC.

Bo He

Haojun Yao

Chengqi Yi

Advances in the joint profiling technologies of 5mC and 5hmC

5mC和5hmC联合分析技术的进展

This review discusses leveraging click chemistry to address unmet needs in PROTAC development.

Ce Yang

Ravi Tripathi

Binghe Wang

Click chemistry in the development of PROTACs

Two pyrene-tetrazole conjugates were synthesized as photoreactive chromophores that allow for the first time the combination of metabolic labelling of DNA in cells and subsequent bioorthogonal "photoclick" modification triggered by visible light. Two strained alkenes and three alkene-modified nucleosides were used as reactive counterparts and revealed no major differences in their "photoclick" reactivity. This is a significant advantage because it allows 5-vinyl-2'-deoxyuridine to be applied as the smallest possible alkene-modified nucleoside for metabolic labelling of DNA in cells. Both pyrene-tetrazole conjugates show fluorogenicity during the "photoclick" reactions, which is a second advantage for cellular imaging. Living HeLa cells were incubated with 5-vinyl-2'-deoxyuridine for 48 h to ensure one cell division. After fixation, the newly synthesized genomic DNA was successfully labelled by irradiation with visible light at 405 nm and 450 nm. This method is an attractive tool for the visualization of genomic DNA in cells with full spatiotemporal control by the use of visible light as a reaction trigger.

Lisa Rieger

Bastian Pfeuffer

Hans-Achim Wagenknecht

Metabolic labelling of DNA in cells by means of the “photoclick” reaction triggered by visible light

细胞中DNA的代谢标记通过可见光触发的“光点击”反应实现

We report the first X-ray crystal structures of mixed chirality α-helices comprising only natural residues as the example of bicyclic and linear membrane disruptive amphiphilic antimicrobial peptides containing seven l - and four d -residues.

Stéphane Baeriswyl

Hippolyte Personne

Ivan Di Bonaventura

Thilo Köhler

Christian van Delden

Achim Stocker

Sacha Javor

Jean-Louis Reymond

A mixed chirality α-helix in a stapled bicyclic and a linear antimicrobial peptide revealed by X-ray crystallography

通过X射线晶体学揭示的混合手性α螺旋：一种交联双环和线性抗菌肽

Post-labelling cleavable substrates for self-labelling protein tags, such as SNAP- and Halo-tags, can be used to study cell surface receptor trafficking events by stripping dyes from non-internalized protein pools. Since the complexity of receptor biology requires the use of multiple and orthogonal approaches to simultaneously probe multiple receptor pools, we report the development of four membrane impermeable probes that covalently bind to either the SNAP- or the Halo-tag in the red to far-red range. These molecules bear a disulfide bond to release the non-internalized probe using the reducing agent sodium 2-mercaptoethane sulfonate (MESNA). As such, our approach allows the simultaneous visualization of multiple internalized cell surface proteins in two colors which we showcase using G protein-coupled receptors. We use this approach to detect internalized group II metabotropic glutamate receptor (mGluRs), homo- and heterodimers, and to reveal unidirectional crosstalk between co-expressed glucagon-like peptide 1 (GLP1R) and glucose-dependent insulinotropic polypeptide receptors (GIPR). In these applications, we translate our method to both high resolution imaging and quantitative, high throughput assays, demonstrating the value of our approach for a wide range of applications.

Kilian Roßmann

Ramona Birke

Joshua Levitz

Ben Jones

Johannes Broichhagen

Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization

用于自标记酶蛋白标记和GPCR共内化探测的红光和远红光可裂解荧光染料

This study uncovers allosteric regulation in the reductase component (C1) of HPA 3-hydroxylase from Acinetobacter baumannii , where HPA binding enhances flavin production while reducing NADH consumption and H₂O₂ formation.

Surawit Visitsatthawong

Piyanuch Anuwan

Narin Lawan

Pimchai Chaiyen

Thanyaporn Wongnate

Mechanistic insights into allosteric regulation of the reductase component of p-hydroxyphenylacetate 3-hydroxylase by p-hydroxyphenylacetate: a model for effector-controlled activity of redox enzymes

对p-羟基苯乙酸通过别构调节p-羟基苯乙酸3-羟化酶还原酶组分的机制见解：氧化还原酶效应物调控活性的模型

We present iridium( iii ) probes for bioorthogonal metabolic labeling, enabling versatile bioimaging applications including super-resolution X-ray fluorescence nanoscopy and photoluminescence lifetime imaging microscopy to advance sialylation studies.

Vincent Rigolot

Clémence Simon

Aude Bouchet

Lucas Lancel

Veronica Di Battista

Dmitry Karpov

Boris Vauzeilles

Corentin Spriet

Michel Sliwa

Sylvain Bohic

Christophe Biot

Cédric Lion

Click-Ready Iridium(III) Complexes as Versatile Bioimaging Probes for Bioorthogonal Metabolic Labeling

点击即用型铱(III)配合物作为生物正交代谢标记的多功能生物成像探针

The design of potent RAS inhibitors benefits from a molecular understanding of the dynamics in KRAS and NRAS and their oncogenic mutants. Here we characterize switch-1 dynamics in GTP-state KRAS and NRAS by

Enrico Rennella

Chrystèle Henry

Callum J. Dickson

Florian Georgescauld

Thomas E. Wales

Dirk Erdmann

Simona Cotesta

Michel Maira

Richard Sedrani

Saskia M. Brachmann

Nils Ostermann

John R. Engen

Lewis E. Kay

Kim S. Beyer

Rainer Wilcken

Wolfgang Jahnke

Dynamic conformational equilibria in the active states of KRAS and NRAS

KRAS和NRAS活性状态中的动态构象平衡

Structure-inspired peptide design and RaPID selection identifies a macrocyclic peptide with immunotherapeutic potential, and inspires development of complement-modulating drugs.

Sebastiaan M.W.R. Hamers

Leoni Abendstein

Aimee L. Boyle

Seino A.K. Jongkees

Thomas H. Sharp

Selection and characterization of a peptide-based complement modulator targeting C1 of the innate immune system

基于肽的补体调节剂对先天免疫系统C1的选择与表征

短名	RSC Chem. Biol.
Journal Impact	4.08
国际分区	CHEMISTRY, MULTIDISCIPLINARY(Q2)

ISSN	2633-0679
h-index	21

涉及主题	生物化学生物化学遗传学物理有机化学核磁共振医学基因工程类计算机科学计算生物学细胞生物学量子力学酶材料科学内科学地质学
出版信息	出版商: Royal Society of Chemistry，出版周期: ，期刊类型: journal，开源期刊: 是
基本数据	创刊年份: 2020，原创研究文献占比: 86.11%，自引率:2.40%， Gold OA占比: 96.89%
期刊费用	$详情
平均审稿周期	网友分享经验：10 Weeks

RSC Chemical Biology

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Advances in the joint profiling technologies of 5mC and 5hmC

Click chemistry in the development of PROTACs

Metabolic labelling of DNA in cells by means of the “photoclick” reaction triggered by visible light

A mixed chirality α-helix in a stapled bicyclic and a linear antimicrobial peptide revealed by X-ray crystallography

Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization

Mechanistic insights into allosteric regulation of the reductase component of p-hydroxyphenylacetate 3-hydroxylase by p-hydroxyphenylacetate: a model for effector-controlled activity of redox enzymes

Click-Ready Iridium(III) Complexes as Versatile Bioimaging Probes for Bioorthogonal Metabolic Labeling

Dynamic conformational equilibria in the active states of KRAS and NRAS

Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization

Selection and characterization of a peptide-based complement modulator targeting C1 of the innate immune system