Acta Pathologica Microbiologica et Immunologica Scandinavica - Section C Immunology

The ability of particle-bound and fluid-phase ligands (IgG, C3b, and ConA) to induce metabolic activation in cytochalasin B-treated human neutrophils was studied. Different activation mechanisms of the neutrophils are proposed, since the activation of cytochalasin B-treated neutrophils is not dependent on whether or not the ligands are particle-bound but not on the nature of ligand per se.

Jan Hed

Olle Stendahl

Acta Pathologica Microbiologica Scandinavica Series C Immunology

METABOLIC ACTIVATION OF HUMAN NEUTROPHILS BY FLUID-PHASE AND PARTICLE-BOUND LIGANDS

流体相和颗粒结合配体对人类中性粒细胞的代谢激活作用

The chemotactic responsiveness of granulocytes obtained from patients with ulcerative colitis was compared to granulocytes from healthy individuals, using the LMAT (Leukocyte Migration Under Agarose Technique). A depression of chemotaxis found in ulcerative colitis patients was seen to be related to the disease activity. This reduction in granulocyte migration could not be corrected by pre-incubation of cell suspensions with tinidazole. The pattern observed for granulocyte chemotaxis in ulcerative colitis was discussed with reference to use as an aid in the differential diagnosis of ulcerative colitis and Crohn's disease.

JUDY BELSHEIM

HÅKAN GNARPE

ÅKE SWAHN

GRANULOCYTE CHEMOTAXIS IN ULCERATIVE COLITIS

溃疡性结肠炎中的粒细胞趋化作用

The distribution of T lymphocyte subsets was determined in 22 patients with endometrial carcinoma stage I. Following surgery and postoperative radiotherapy the patients had been randomized® into two groups: group A patients received no further treatment, whereas group B patients were treated with 17 a-hydroxyprogesterone caproate for one year. T cells and T cell subsets were estimated by an indirect immunofluorescence technique using the monoclonal antibodies UCHT3 (mature T cells), OKT4 (T helper cells), and OKT8 (T suppressor/cytotoxic cells). When examined 3–12 months after randomization the two groups had similar numbers of UCHT3+ cells and OKT4+ cells. Group B patients, however, had a significantly lower proportion of OKT8+ cells and hence a significantly higher OKT4/OKT8 ratio than group A patients. It is concluded that progestogen therapy has some immunomodulating effects.

MATHIAS ONSRUD

STEIN KVALEY

INFLUENCE OF PROGESTOGEN THERAPY ON T LYMPHOCYTE SUBSETS

孕激素治疗对T淋巴细胞亚群的影响

Acta Pathologica Microbiologica Scandinavica Series C: ImmunologyVolume 92C, Issue 1-6 p. 139-143 INTERACTION OF HUMAN SERUM TRANSFERRIN WITH NEISSERIA GONORRHOEAE, DEMONSTRATED BY AGGLUTINATION AND PRECIPITATION TERJE O. RØD, TERJE O. RØD Department of Microbiology, Faculty of Medicine, University of Trondheim, Trondheim. NorwaySearch for more papers by this authorJOHAN A. MÆLAND, Corresponding Author JOHAN A. MÆLAND Department of Microbiology, Faculty of Medicine, University of Trondheim, Trondheim. NorwayDepartment of Microbiology, Faculty of Medicine, University of Trondheim, N-7000 Trondheim, Norway.Search for more papers by this author TERJE O. RØD, TERJE O. RØD Department of Microbiology, Faculty of Medicine, University of Trondheim, Trondheim. NorwaySearch for more papers by this authorJOHAN A. MÆLAND, Corresponding Author JOHAN A. MÆLAND Department of Microbiology, Faculty of Medicine, University of Trondheim, Trondheim. NorwayDepartment of Microbiology, Faculty of Medicine, University of Trondheim, N-7000 Trondheim, Norway.Search for more papers by this author First published: November 1984 https://doi.org/10.1111/j.1699-0463.1984.tb00065.xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Human sera show non-immune percipitation with ultrasonically disrupted bacteria of the N. gonorrhoeae strain 8551. A human serum was resolved by DEAE cellulose chromatography and immunosorbence to isolate the serum component involved in the precipitation. Testing of the final product against antisera to various serum proteins and analysis by SDS-PAGE revealed only serum transferrin. This preparation and transferrin obtained commercially produced precipitation in agar gel against the gonococcal extract. The bacterium was agglutinated by transferrin in solution or when carried by latex particles, and particles sensitized with the bacterial extract were agglutinated by transferrin. Four out of 26 gonococcal strains examined showed reactivity with transferrin in the precipitation and agglutination tests. Volume92C, Issue1-6November 1984Pages 139-143 RelatedInformation

TERJE O. RØD

JOHAN A. MAELAND

INTERACTION OF HUMAN SERUM TRANSFERRIN WITH NEISSERIA GONORRHOEAE, DEMONSTRATED BY AGGLUTINATION AND PRECIPITATION

人血清转铁蛋白与淋病奈瑟菌的相互作用：通过凝集和沉淀法证明

Human blood lymphocytes were stimulated in vitro by four Staphylococcus aureus strains. Activation of immunoglobulin-secreting cells was determined by a reverse plaque forming cell (PFC) assay, and proliferation by quantitation of thymidine incorporation. Whole killed S. aureus were slightly more efficient than water-soluble preparations in the form of sonicated extracts and culture supernatants. Two S. aureus strains rich in protein A (Cowan I and E 2371) and one S. aureus strain deficient in protein A (E 1369) were potent B-lymphocyte stimulators inducing maximal activity on day 6 of culture. Another S. aureus strain deficient in protein A (Wood 46) did not possess++ human lymphocyte stimulating capacity.

H. EFFERSØE

F. ESPERSEN

V. ANDERSEN

INDUCTION OF IMMUNOGLOBULIN SECRETION IN CULTURED HUMAN LYMPHOCYTES BY 4 STAPHYLOCOCCUS AUREUS STRAINS AND THEIR EXTRACTS

4株金黄色葡萄球菌及其提取物对培养人淋巴细胞免疫球蛋白分泌的诱导作用

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METABOLIC ACTIVATION OF HUMAN NEUTROPHILS BY FLUID-PHASE AND PARTICLE-BOUND LIGANDS

GRANULOCYTE CHEMOTAXIS IN ULCERATIVE COLITIS

INFLUENCE OF PROGESTOGEN THERAPY ON T LYMPHOCYTE SUBSETS

INTERACTION OF HUMAN SERUM TRANSFERRIN WITH NEISSERIA GONORRHOEAE, DEMONSTRATED BY AGGLUTINATION AND PRECIPITATION

INDUCTION OF IMMUNOGLOBULIN SECRETION IN CULTURED HUMAN LYMPHOCYTES BY 4 STAPHYLOCOCCUS AUREUS STRAINS AND THEIR EXTRACTS

METABOLIC ACTIVATION OF HUMAN NEUTROPHILS BY FLUID-PHASE AND PARTICLE-BOUND LIGANDS

GRANULOCYTE CHEMOTAXIS IN ULCERATIVE COLITIS

INFLUENCE OF PROGESTOGEN THERAPY ON T LYMPHOCYTE SUBSETS

INTERACTION OF HUMAN SERUM TRANSFERRIN WITH NEISSERIA GONORRHOEAE, DEMONSTRATED BY AGGLUTINATION AND PRECIPITATION

INDUCTION OF IMMUNOGLOBULIN SECRETION IN CULTURED HUMAN LYMPHOCYTES BY 4 STAPHYLOCOCCUS AUREUS STRAINS AND THEIR EXTRACTS