- Biochemical and Bioprocess Engineering (for food production, fortification, safety, etc)  - Metabolic and Genetic Engineering in Food Science  - Food Microbiology  - Nanobiotechnology in Food Science and Technology  - Biopolymers as Food Packaging Materials  - Role of Microorganisms in Waste Treatment of Food Industries

The journal "Applied Food Biotechnology" covers the following areas: Biochemical and Bioprocess Engineering for food production, fortification, and safety; Metabolic and Genetic Engineering in Food Science; Food Microbiology; Nanobiotechnology in Food Science and Technology; Biopolymers as Food Packaging Materials; and the Role of Microorganisms in Waste Treatment of Food Industries.

《Applied Food Biotechnology》期刊涵盖以下领域：食品生产、强化和安全的生化与生物过程工程；食品科学中的代谢与遗传工程；食品微生物学；食品科学与技术中的纳米生物技术；作为食品包装材料的生物聚合物；微生物在食品工业废物处理中的作用。

Applied Food Biotechnology

Graphical invitation Aims and Scope This special issue of Applied Food Biotechnology will publish frontier research articles, short communications and reviews on all the latest advances on Prebiotics, Probiotics & Synbiotics. Subtopics: Microbiota, food enrichment and characterization, prebiotics, screening of new probiotics, probiotics survival, functional food, synbiotics, etc. Keywords: Prebiotics, Probiotics, Synbiotics

Arely Prado-Barragán

Gpe. Virginia Nevarez-Moorillón

Applied food biotechnology

Scope of special issue includes "Prebiotics, Probiotics and Synbiotics"

特刊范围包括“益生元、益生菌和合生元”

本期特刊范围包括“益生元、益生菌和合生元”

Background and Objective:
Edible film is one of the solutions for food packaging that carry antimicrobial and antioxidant compounds, usually found in Medinilla speciosa fruits. The objective of this study was to assess effects of Medinilla speciosa fruit extract on physical, chemical and bioactivity of edible films as well as effects of coating on sausage quality during storage.
Material and Methods:
The edible film included 2% w w-1 chitosan, 2% w w-1 sorbitol and Medinilla speciosa fruit extract. Variations of Medinilla speciosa extract included 0, 2.5, 5 and 10% (w w-1), while the storage temperature included 4 and 27 oC. Seven parameters of edible film characteristics were assessed, including tensile strength, elongation, water vapor permeability, antimicrobial and antioxidant activities, surface microstructure and Fourier-transform infrared response. Parameters assessed in storage treatment included total plate count, yeast mold count and thiobarbituric acid reactive substances. Data were analyzed using Kruskal-Wallis test. Organoleptic characteristics were analyzed using Friedman test and SPSS Software.
Results and Conclusion:
Results showed that the higher the concentration of Medinilla speciosa extract was, the higher the value of tensile strength, water vapor permeability and antioxidant activity and lower the elongation value were. The film control with 0% Medinilla speciosa extract was the only film that met Japanese standard for the water vapor permeability value, including 6.74 gm-2 h-1. Furthermore, shelf life of sausages coated with edible films revealed that the higher the concentration of Medinilla speciosa extract was, the lower the total plate count, yeast mold count and thiobarbituric acid reactive substances values were. Study demonstrated that the Medinilla speciosa extract edible film inhibited microbiological and oxidative damages. ​Oon Day 15 of storage, sausages coated with edible films with 10% Medinilla speciosa extract included lower total plate count, yeast mold count and thiobarbituric acid reactive substances values, respectively including 2.4 ±0.02 log CFU g-1, 1.3 ±0.08 log CFU g-1 and 13.38 ±0.22 mg malonaldehyde kg-1 sausage, compared to the control film. Organoleptic assessment showed no major differences in consumer acceptance. In conclusion, edible film with 10% Medinilla speciosa extract is the best physical, chemical and bioactivity film. Moreover, this film extends the sausage shelf life.
Conflict of interest: The authors declare no conflict of interest.

Dwi Retno Fatmawati

Artini Pangastuti

Ari Susilowati

Use of Edible Film Incorporated with Parijoto Fruit Extract (Medinilla speciosa Blume) to Inhibit Microbiological and Oxidative Damages of Sausages

使用含帕里乔托果提取物（美丁花）的可食用膜抑制香肠的微生物和氧化损伤

Background and Objective:
Ganoderma lucidum is one of the medicinal fungi frequently used as supplement. The intracellular polysaccharides of this fungus include high molecular weights and help strengthen the immune system. Furthermore, these polysaccharides act as antioxidants by inhibiting free radicals and enhancing activity of the enzymes. Addition of various elicitors to the fungi submerged culture media affects the cell growth and metabolite production. Fungal extracts are one of these elicitors.
Material and Methods:
In this study, Ganoderma lucidum was first cultured in various culture media to investigate the base media. Using three various methods (soaking the fruit body in water, disintegrating the fruit body with a blender and boiling the fruit body), Agaricus bisporus fruit body extract was prepared as elicitor and the extract with the highest sugar content was used. For the optimization of growth and antioxidant activity of the intracellular polysaccharides, effects of six independent factors were investigated using Placket-Burman method, including Agaricus bisporus extract, peptone, maltose, pH, vitamin B1 and CaCl2. Response surface method was used to optimize three factors of vitamin B1, Agaricus bisporus extract and maltose. Then, stirred tank bioreactor was used to culture Ganoderma lucidum.
Results and Conclusion:
The YPG culture medium was selected as the base medium based on mycelial growth and antioxidant activity of the intracellular polysaccharides (IC50). Sugar content of the Agaricus bisporus extract was 30.66 µg.ml-1. Placket-Burman method revealed that the extracts of Agaricus bisporus, maltose and vitamin B1 significantly increased antioxidant activity of the intracellular polysaccharides. After optimizing these factors using RSM, the IC50 was reported as 1.047 mg.ml-1. Ganoderma lucidum cultivation in bioreactor significantly increased the cell growth (5.29 g.l-1). Intracellular polysaccharides included an IC50 of 1.14 mg.ml-1, which was significantly higher than that the intracellular polysaccharides included in YPG culture media.
Conflict of interest: The authors declare no conflict of interest.

Maryam Esmaelifar

Ashrafalsadat Hatamian-Zarmi

Hale Alvandi

Majid Azizi

Zahra Beagom Mokhtari-Hosseini

Bahman Ebrahimi-Hoseinzadeh

Optimization of Antioxidant Activities and Intracellular Polysaccharide Contents Using Agaricus bisporus Extract as Elicitor in Submerged Fermenting Ganoderma lucidum

优化抗氧化活性和胞内多糖含量：以双孢蘑菇提取物为激发子在深层发酵灵芝中的应用

Background and Objective:
Food wastes cause economic losses and environmental problems. Hence, ability to transform food wastes into high-value added products is highly attractive. The aim of this study was to produce melanin pigments by fermentation that include wide potential uses in agriculture, cosmetics and pharmaceutical industries using domestic wastes such as melon peel, watermelon peel and carrot peel and industrial by-products such as whey and molasses.
Material and Methods:
Two Aureobasidium pullulans strains were assessed for melanin production. Fourier-transform infrared spectroscopy, scanning electron microscope, zeta potential, ultraviolet absorbance and solubility assays were carried out to characterize produced melanin nanoparticles.
Results and Conclusion:
The highest intracellular (0.19 g l-1) and extracellular (3.52 g l-1) melanin concentrations were produced by Aureobasidium pullulans NBRC 100716 using carrot peel extracts as fermentation media. Results of characterization were compared with those of synthetic melanin used as standard and the produced nanoparticles were validated. Particle sizes of the nanoparticles ranged 10-760 nm with negative charges, as suggested by previous literature. Results showed that carrot peel was a good candidate, which could be used for the production of high value-added melanin. When carrot peel extract was used as a fermentation medium, characteristics of the melanin produced by Aureobasidium pullulans NBRC 100716 strain were similar to those of synthetic melanin.
Conflict of interest: The authors declare no conflict of interest

Gamze Nur Mujdeci

Natural Melanin Synthesized by Aureobasidium pullulans Using Food Wastes and its Characterization

利用食品废弃物由出芽短梗霉合成的天然黑色素及其特性

Background and Objective: Conventional applied techniques used for detecting pathogenic microorganisms that generally are based on plating, serological and biochemical assays are unreliable and expensive while lacking sensitivity and specificity compared to new analytical methods. Investigation of reliable and rapid analytical diagnosis methods seems a necessity today. In the present study, a high accurate method was developed aiming to pre-concentrate and improve identification of Staphylococcus aureus as a major bacterial human pathogen by using a molecular imprinted polymer (MIP) based membrane.
Materials and Methods: Cellulose acetate was used as the basic membrane with a pore size of 1.2 μm, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linking monomer, antibody buffer medium as the template molecule, and 2,2'-Azobis(2-methylpropionitrile) as the initiator agent. After selecting the best membrane composition resulting from the optimum ratio of antibody to imprinted monomer, electron microscopy testing was used to evaluate the characterization and stabilization of the molecular imprinting of templates on the membrane.
Results and Conclusion: According to the results, the suspension of Staphylococcus aureus with a dilution of 3×105 after being adjacent to MIPs modified membranes had the highest bacterial mass absorption in MIP4 filter and reduced to a level of 1.3×104. The manufactured nano membrane could lead to a significant development in quality control of food industry compared to traditional methods due to a very shorter required time of bacterial mass diagnosis with a very higher accuracy.
Keywords: Antibody; Molecular Imprinted Polymer; Nano Membrane; Staphylococcus aureus

Sogand Vahid

Hamed Ahari

Behrouz Akbari Adergani

Seyedeh Fatemeh Seyed Reihani

Designing a Molecularly Imprinted Polymer-Based Nanomembrane for the Selective Removal of Staphylococcus aureus from Aqueous Media

设计一种基于分子印迹聚合物的纳米膜用于选择性去除水介质中的金黄色葡萄球菌

Background and Objective:
Black soybean (Glycine max (L.) Merr.) Detam-1 variety includes high quantities of isoflavone majorly in glucoside form; in which, the biochemical antioxidant activity was lower than that in isoflavone aglycone form. Fermentation by lactic acid bacteria can increase antioxidant activity and isoflavone aglycone of black soymilk. However, the biochemical ability is strain-dependent and sucrose or skim milk supplementation during processing may affect this ability. The objective of the current study was to investigate antioxidant properties of the fermented black soymilk and fermented black soymilk supplemented with 2% sucrose or skim milk using three Indonesian indigenous lactic acid bacteria, namely Lactobacillus plantarum WGK 4, Streptococcus thermophilus Dad 11 and Lactobacillus plantarum Dad 13. Furthermore, cell growth and acid production were investigated.
Material and Methods:
Fermentation of black soymilk and black soymilk supplemented with 2% sucrose or skim milk was carried out using three indigenous lactic acid bacteria at 37 °C for 18 h. Viable cell, pH, titratable acidity, β-glucosidase activity, isoflavone aglycone, total phenolic content and antioxidant activity of black soymilk were assessed at the beginning of the experiment and after 18 h of fermentation.
Results and Conclusion:
Results showed that all strains could grow (9 log CFU ml-1) and produce acid in black soymilk and black soymilk supplemented with 2% sucrose or skim milk. Fermentation increased isoflavone aglycone through β-glucosidase activity, which resulted in increased total phenolic content and antioxidant activity. Fermented black soymilk with no sucrose or skim milk exhibited the highest β-glucosidase activity (19.66-21.54 mU ml-1), daidzein formation (62-74%), genistein formation (67-80%) and antioxidant capacity (32.81-38.47%). All three lactic acid bacteria strains enhanced antioxidant activity and isoflavone aglycone of the black soymilk. Sucrose or skim milk addition did not affect the cell growth but increased acid production and decreased β-glucosidase activity and isoflavone aglycone formation. These three lactic acid bacteria included similar abilities to enhance antioxidant activity and isoflavone aglycone formation in fermented black soymilk.

Benediktus Yudo Leksono

Muhammad Nur Cahyanto

Tyas Utami

Antioxidant Activity of Isoflavone Aglycone from Fermented Black Soymilk Supplemented with Sucrose and Skim Milk Using Indonesian Indigenous Lactic Acid Bacteria

Abbreviation	Appl. Food Biotechnol.
Journal Impact	2.47
Quartiles(Global)	FOOD SCIENCE & TECHNOLOGY(Q2)

ISSN	2345-5357, 2423-4214
h-index	24

Publication Information	Publisher: National Nutrition and Food Technology Research Institute，Publishing cycle: ，Journal Type: journal，Open Access Journals: Yes
Basic data	Year of publication: 2014，Proportion of original research papers: 88.46%，Self Citation Rate:53.80%， Gold OA Rate: 0.00%
Journal Fees	$200Details
Average review cycle	网友分享经验：9 Weeks

Applied Food Biotechnology

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Scope of special issue includes "Prebiotics, Probiotics and Synbiotics"

Scope of special issue includes "Prebiotics, Probiotics and Synbiotics"

Scope of special issue includes "Prebiotics, Probiotics and Synbiotics"

Scope of special issue includes "Prebiotics, Probiotics and Synbiotics"

Scope of special issue includes "Prebiotics, Probiotics and Synbiotics"

Use of Edible Film Incorporated with Parijoto Fruit Extract (Medinilla speciosa Blume) to Inhibit Microbiological and Oxidative Damages of Sausages

Optimization of Antioxidant Activities and Intracellular Polysaccharide Contents Using Agaricus bisporus Extract as Elicitor in Submerged Fermenting Ganoderma lucidum

Natural Melanin Synthesized by Aureobasidium pullulans Using Food Wastes and its Characterization

Designing a Molecularly Imprinted Polymer-Based Nanomembrane for the Selective Removal of Staphylococcus aureus from Aqueous Media

Antioxidant Activity of Isoflavone Aglycone from Fermented Black Soymilk Supplemented with Sucrose and Skim Milk Using Indonesian Indigenous Lactic Acid Bacteria