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Conjugation of plasmids from Escherichia coli is essential for the genetic manipulation of Streptomyces spp. To facilitate intergeneric conjugation from E. coli to Streptomyces the conjugative machinery required for genetic transfer is usually provided by the non-transferable helper plasmid, pUZ8002. Here we present the complete nucleotide sequence of pUZ8002, describe the previously undocumented creation process, and provide details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence.

Daniel E. Larcombe

Robyn E. Braes

James T. Croxford

James W. Wilson

David H. Figurski

Paul A. Hoskisson

Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002

链霉菌间基因转移辅助质粒pUZ8002的序列及起源

Syeda Wardah Noor

Sara Arshad

Syed Muhammad Abdullah

Bushra Khudadad Kayani

Sahar Fazal

16S rRNA gene sequencing reveals bacterial diversity in Khewra Salt Mine walls

16S rRNA基因测序揭示凯沃拉盐矿壁中的细菌多样性

A potentially new species of Streptomyces was isolated from station 177 of the Sundarbans Seasonal Time Series in the Indian Sundarbans mangrove. The isolate was cultured from a sediment sample on TYS medium of salinity 15. Sequencing and annotation of the 16S rRNA showed 100% identity against S. laurentii NPS17 against GenBank/ENA/DDBJ. Annotation of the whole genome against the GTDB database showed closest identity with S. terrae SKN60 and belongs to the same clade as S. roseicoloratus TRM44457T and S. laurentii ATCC 31255. The genome is ~7.2 Mb and has a G+C% of 73%. The average amino acid identity was 85.01% with S. roseicoloratus and 80.34% with S. roseolus . The assembly reflected the presence of all essential genes and had 19 biosynthetic gene clusters predicted.

Anwesha Ghosh

Simran Kaur Bhambra

Raghu Chandrasekaran

Punyasloke Bhadury

Genome description of a potentially new species of Streptomyces isolated from the Indian Sundarbans mangrove

从印度孙德尔本斯红树林分离出的可能为新种的链霉菌的基因组描述

Salmonella enterica serovar Typhi primarily persists in chronic carriers by forming biofilms on gallstones in the gallbladder. We have developed a gallstone mouse model to study chronic carriage. To better understand the infection timeline and differentiate between mice that have maintained long-term gallbladder carriage from those that have cleared infection, we utilized bioluminescent S . Typhimurium and in vivo imaging to detect and track the organ-specific presence of bacteria in living mice. The mice infected with our bioluminescent S . Typhimurium showed luminescence in the abdomen as early as 3 days in comparison to the mice infected with non-luminescent WT S . Typhimurium. With our methods, we achieve image resolution such that we can confidently identify the presence of S . Typhimurium in the gallbladder at &gt;60 days post-infection. Using these methods, we have determined that the minimum number of bacteria necessary to detect luminescence in the mice is 10 3 c.f.u. and that one out of six initially infected mice will remain persistently infected for greater than 60 days, with gallbladder bacterial loads reaching upwards of 10 3 per milligram of tissue. Given that our limit of detection of luminescence is 10 3 c.f.u., our sensitivity is robust enough to identify the bacterial loads present in the average chronically infected mouse. The quantification of individual organs’ bacterial c.f.u. and comparison of luminescence between WT and luminescent S . Typhimurium validate that our technique is specific and sensitive enough to detect organ-specific infection in our model of typhoidal chronic carriage.

Aliyah N. Bennett

Baileigh Laipply

John S. Gunn

Methods for detecting and monitoring Salmonella infection and chronic carriage in living mice using bioluminescent in vivo imaging

利用生物发光活体成像检测和监测活体小鼠中沙门氏菌感染及慢性携带的方法

The use of membrane-specific dyes for in vivo fluorescent microscopy is commonplace. However, most of these reagents are non-specific and cannot track specific lipid species movement, instead often acting as non-covalent lipid-associated probes or requiring the uptake of whole lipids and acyl tails into the membrane. This issue has been solved in eukaryotic cell biology by the use of click-chemistry-liable phospholipid headgroup pulse labels. Here, we describe a method for in vivo phospholipid labelling by fluorescent imaging in Pseudomonas aeruginosa using a phosphatidylcholine mimic, ‘propargyl-choline’ (PCho). This click-chemistry-liable headgroup mimic is visible by microscopy and allows the covalent labelling of lipids. Fluorescence of the cell membranes, visible in heterogeneous patches, is dependent on PCho concentration and is localized in the membrane fraction of cells, demonstrating that it is suitable for membrane labelling and cell imaging.

Chris L.B. Graham

Jack Bryant

David I. Roper

Manuel Banzhaf

Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline

使用磷脂酰胆碱模拟物炔丙基胆碱对铜绿假单胞菌PAO1的膜染色和磷脂追踪

We announce the deposition of the first two enterotoxigenic Escherichia coli (ETEC) genomes from Malawi. They were isolated from the faeces of asymptomatically infected children obtained in 2014. Both genomes encode the porcine variant of the heat-labile toxin and no known ETEC colonization factors.

Publication Information	Publisher: Microbiology Society，Publishing cycle: ，Journal Type: journal，Open Access Journals: Yes
Basic data	Year of publication: 2019，Proportion of original research papers: 0.00%，Self Citation Rate:N.A.， Gold OA Rate: 0.00%
Journal Fees	$Details
Average review cycle	网友分享经验：12 Weeks

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Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002

16S rRNA gene sequencing reveals bacterial diversity in Khewra Salt Mine walls

Genome description of a potentially new species of Streptomyces isolated from the Indian Sundarbans mangrove

Methods for detecting and monitoring Salmonella infection and chronic carriage in living mice using bioluminescent in vivo imaging

Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline

16S rRNA gene sequencing reveals bacterial diversity in Khewra Salt Mine walls

Genome description of a potentially new species of Streptomyces isolated from the Indian Sundarbans mangrove

Methods for detecting and monitoring Salmonella infection and chronic carriage in living mice using bioluminescent in vivo imaging

Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline

Enterotoxigenic Escherichia coli in Blantyre, Malawi

ISSN	2516-8290
h-index	2