细胞和组织库提供了一个向参与细胞和组织库和移植的科学家和临床医生传播信息的论坛。 CELL AND TISSUE BANKING 是一份国际同行评审期刊，发表以下领域的原创论文：关于组织银行一般方面的基础研究，例如储存细胞/组织的质量保证和控制、保存和灭菌方法对细胞/组织、生物技术等；储存细胞/组织的临床应用；细胞/组织采购、加工、储存和分配的实践标准；伦理道德问题;法医问题。

Cell and Tissue Banking is an international peer-reviewed journal that provides a forum for scientists and clinicians involved in cell and tissue banking and transplantation to disseminate information. The journal publishes original research papers in the following areas: fundamental research on general aspects of tissue banking, such as quality assurance and control for the storage of cells/tissues, the effects of preservation and sterilization methods on cells/tissues, biotechnology, etc.; clinical applications of stored cells/tissues; practical standards for the procurement, processing, storage, and distribution of cells/tissues; ethical issues; and forensic matters.

《Cell and Tissue Banking》是一个国际同行评审期刊，旨在为参与细胞和组织库及移植的科学家和临床医生提供一个信息交流的平台。期刊发表原创研究论文，涵盖以下领域：关于组织银行的基础研究，包括细胞和组织的质量保证与控制、保存和灭菌方法对细胞和组织的影响、生物技术等；细胞和组织的临床应用；细胞和组织的采购、加工、储存和分配的实践标准；伦理道德问题以及法医问题。

Cell and Tissue Banking

Wound healing is a complex process that involves interaction of soluble mediators, extracellular matrix and infiltrating blood cells. Chronic and non-healing skin defects contribute significantly to morbidity and mortality of many patients. Recently, despite the current medical progress, the chronic and non-healing wounds still represent a serious medical problem. In many cases, conventional therapeutic approaches, such as dermal substitutes and growth factor therapy failed and do not produce the expected results, patients are exposed to a high risk of infection, sepsis or amputation. For that reason clinicians and researchers are forced to searching for alternative methods to induce healing process which may result into complete wound closure. Mesenchymal stem cells (MSCs) represent a unique tool of tissue engineering and regenerative medicine and a promising therapeutic strategy. Due to their unique biological properties, MSCs seem to be the perspective modality method for these patients. Many preclinical and clinical studies suggest the possibility of using these cells in tissue regeneration, healing acute and chronic wounds and scar remodelling. The objective of the present review is to summarize the current information and preclinical data about MSCs, their biological characteristics and mode of action during regenerative and healing processes, as well as their clinical application in chronic wounds treatment.

Peter Zahorec

Jan Koller

Lubos Danisovic

Martin Bohac

Cell and tissue banking

Cell Tissue Bank

Mesenchymal stem cells for chronic wounds therapy.

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are life-threatening diseases in critically ill patients. Although pathophysiology of ALI/ARDS has been investigated in many studies, effective therapeutic strategies are still limited. Mesenchymal stem cell (MSC)-based therapy is emerging as a promising therapeutic intervention for patients with ALI. During the last two decades, researchers have focused on the efficacy and mechanism of MSC application in ALI animal models. MSC derived from variant resources exhibited therapeutic effects in preclinical studies of ALI with different mechanisms. Based on this, clinical studies on MSC treatment in ALI/ARDS has been tried recently, especially in COVID-19 caused lung injury. Emerging clinical trials of MSCs in treating COVID-19-related conditions have been registered in past two years. The advantages and potential of MSCs in the defense against COVID-19-related ALI or ARDS have been confirmed. This review provides a brief overview of recent research progress in MSC-based therapies in preclinical study and clinical trials in ALI treatment, as well as the underlying mechanisms.

Jinfeng Liang

Weiyou Dai

Shihang Xue

Feifei Wu

Enhai Cui

Ruolang Pan

Recent progress in mesenchymal stem cell-based therapy for acute lung injury

间充质干细胞治疗急性肺损伤的最新进展

Parkinson disease (PD) is considered as one of the most worldwide neurodegenerative disorders. The major reasons associated to neurodegeneration process of PD pathogenesis are oxidative stress. Many studies reported that natural antioxidant molecules, especially, curcumin can suppress inflammatory pathways and preserve dopaminergic neurons damage in PD. Further, the poor pharmacokinetics, instability of chemical structure because of fast hydrolytic degradation at physiologic condition and especially, the presence of the blood brain barrier (BBB) has regarded as a considerable restriction factor for transfer of neurotherapeutic molecules to the brain tissue. The present research aims to the fabrication of nanoformulated curcumin loaded human endometrial stem cells derived exosomes (hEnSCs EXOs-Cur) to study on enhancing curcumin penetration to the brain across BBB and to improve anti- Parkinsonism effects of curcumin against neural death and alpha-synuclein aggregation. hEnSCs EXOs-Cur characterization results demonstrated the accurate size and morphology of formulated curcumin loaded exosomes with a proper stability and sustained release profile. In vivo studies including behavioral, Immunohistochemical and molecular evaluations displayed that novel formulation of hEnSCs EXO-Cur is able to cross BBB, enhance motor uncoordinated movements, suppress the aggregation of αS protein and rescue neuronal cell death through elevation of BCL2 expression level as an anti-apoptotic protein and the expression level reduction of BAX and Caspase 3 as apoptotic markers.

Mahsa Mobahat

Esmaeil Sadroddiny

Vajihe Taghdiri Nooshabadi

Somayeh Ebrahimi-Barough

Arash Goodarzi

Ziba Veisi Malekshahi

Jafar Ai

Curcumin-loaded human endometrial stem cells derived exosomes as an effective carrier to suppress alpha-synuclein aggregates in 6OHDA-induced Parkinson's disease mouse model.

姜黄素负载人子宫内膜干细胞来源外泌体作为有效载体抑制6OHDA诱导的帕金森病小鼠模型中α-突触核蛋白聚集。

Amniotic membrane is arguably one of the most popular biological wound dressings on the market today. Various growth factors and cytokines inherent to amniotic membrane tissue have been recognized as key mediators in wound healing and tissue regeneration, giving the tissue its clinical utility. Sterilization methodologies using irradiation are recognized as the gold standard in the field and routinely used to prepare tissue allografts, including amniotic membrane for transplantation. However, irradiation is not always compatible in preserving the physical structure or biochemical factors of biological materials and can potentially result in detrimental effects to the critical quality attributes of allograft tissues. Alternatively, a novel sterilization technique involving supercritical carbon dioxide (SCCO2) has been shown to have minimal effect on the inherent biophysical properties of sensitive biological tissues and tissue-derived products. At BioBridge Global, we have developed a process utilizing SCCO2 technology for the sterilization of an amniotic membrane tissue allograft product. This process, first and foremost, meets industry standards for sterilization while simultaneously maintaining the biochemical composition of the tissue. Our results show that upon SCCO2 sterilization, most of the growth factors tested were conserved, with many at quantities significantly greater than commercially available gamma and electron beam irradiated tissue. The SCCO2-sterilized amniotic membrane allograft is unique in that it is designed to overcome limitations associated with traditional tissue sterilization methodologies, namely, the conservation of key biological factors inherent to native amniotic membrane tissue. It is anticipated that by retaining these biological factors, clinical outcomes associated with the use of SCCO2-sterilized amniotic membrane will be improved.

Jennifer O’Connell

Komali Pentakota

Donny Villeareal

Jose Faz

Xiaoli Li

Anthony Trinh

Rachel Beddard

Scott Jones

Anand Srinivasan

Development of a sterilization process for amniotic membrane allograft tissue using supercritical carbon dioxide and NovaKill

利用超临界二氧化碳和NovaKill开发羊膜同种异体移植物组织灭菌工艺

Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitoring tumors dynamically with exosomes, to facilitate clinical and basic scientific research. The osteosarcoma biological specimen and clinical data of osteosarcoma were collected in Ruijin Hospital in two stages. In the first stage (2015-2017), the collection of tissue specimens and blood samples were performed at diagnostic biopsy, definitive surgery, recurrence and lung metastasis, according to the Children's Oncology Group protocol. In the second stage (2017-2019), the tissue specimens were collected the same as before, but the blood samples were collected at the beginning of each MAP-I (methotrexate, cisplatin, doxorubicin, ifosfamide) chemotherapy cycle, and every 6 months after the last chemotherapy up to 3 years, according to our modified protocol, to dynamically monitor the status of possible alteration of gene expression profiling in the osteosarcoma. A total of 268 patients with osteosarcoma were enrolled in this study, 161 were men and 107 were women, with the mean age of 24.51 ± 15.58 years. Local recurrence occurred in 29 patients and lung metastasis in 51. The numbers of tissue and blood specimens reached 360 and 1023, respectively. 11 specimens were from recurrent osteosarcoma and 25 were from lung metastasis. The corresponding clinical and demographic data were collected in our electronic database. The osteosarcoma biobank built with our modified protocol mentioned above has the potential of monitoring tumors dynamically with exosomes and could provide specimens to the researches improving the biological understanding and outcome of this disease.

Shizhao Zang

Jun Wang

Junxiang Wen

Qiyuan Bao

Yuhui Shen

Weibin Zhang

Establishment of a dynamic osteosarcoma biobank: Ruijin experience.

建立动态骨肉瘤生物样本库：瑞金经验。

Non-healing wounds cost the National Health Service over £5.6 billion annually in wound management. Skin allografts are used to treat non-healing wounds, ulcers and burns, offering the best protection against infection. In order to allow host cells to repopulate and to avoid immunogenicity, cell components are removed through decellularisation. Decellularisation of human dermis has so far been performed in NHS Blood and Transplant using a combination of two enzymes (RNase T1 and the recombinant human DNase Pulmozyme)®. This study aims at validating a new method to remove DNA from donated dermis via the use of a single enzyme, Benzonase, known for its effectiveness of DNA digestion. Skin samples were decellularised by removing the epidermis, lysing of dermal cells, removal of cellular fragments by a detergent wash and removal of nucleic acids by a nuclease incubation with either Benzonase or Pulmozyme + RNase T1. DNA quantification with PicoGreen, as well as histology on wax-embedded biopsies, stained with DAPI and haemotoxylin and eosin, were performed. In vitro toxicity test on human osteosarcoma immortalised cells and skin fibroblasts, and biomechanical (tensile) testing, were also performed. The effectiveness of DNA digestion with the new methodology was comparable to previous procedure. Mean DNA removal percentage following decellularisation with Pulmozyme + RNase was 99.9% (3.83 ng/mg). Mean DNA removal percentage with Benzonase was 99.8% (9.97 ng/mg). Histology staining showed complete decellularisation following either method. Benzonase was proven to be non-toxic to both cell lines used, and a one-way Anova test showed no significant difference in neither stress nor strain between acellular dermal matrix decellularised with either Benzonase or Pulmozyme + RNase T1. Benzonase was able to effectively decellularise dermis after prior removal of epidermis. It performed just as well as the combination of Pulmozyme + RNase T1, but represents significant advantages in terms of cost effectiveness, procurement and storage; Benzonase has been successfully used in the decellularisation of other tissues, thus would be better for Tissue Banking use. Switching to this combined DNase/RNase can have far-reaching consequences in the production of acellular human dermal matrix by NHSBT and in the treatment of patients requiring it.

Irit Stern

Valentina Barrera

Michael Randles

Paul Rooney

Advances in preparation of acellular human dermis for tissue banking and transplantation

无细胞人真皮制备在组织库与移植中的进展

This study aimed to investigate whether extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ASCs) promote skin wound healing by delivering basic fibroblast growth factor (bFGF) to enhance vascular endothelial growth factor (VEGF) expression. ASCs were isolated and transfected with either a bFGF knockdown lentivirus (Lv-sh-bFGF) or a control lentivirus (Lv-sh-NC). EVs were extracted from ASCs cultures and characterized by transmission electron microscopy, nanoparticle tracking analysis, and Western blotting for surface markers. EVs were extracted from the conditioned mediums of ASCs and subjected to different treatments. These EVs or control treatments were injected at the wound edges. Wound healing was assessed using histological techniques, including H&E and Masson's trichrome staining to evaluate tissue regeneration, collagen organization, and immunohistochemistry for CD31 to quantify microvessel density. Protein expression of bFGF and VEGF was measured by Western blotting. ASC-derived EVs significantly promoted angiogenesis and improved skin wound healing. EVs encapsulating bFGF enhanced VEGF expression in the wound tissue, while knockdown of bFGF reduced both bFGF and VEGF expression, leading to delayed wound healing. Further knockdown of VEGF partially reversed the pro-angiogenic and wound-healing effects of bFGF-encapsulated EVs. This study demonstrates that ASC-derived EVs promoted skin wound repair by enhancing angiogenesis and accelerating tissue regeneration through the bFGF/VEGF axis. These findings highlight the therapeutic potential of ASCs-derived EVs in improving skin wound healing.

Yonghu Ding

Mengsheng Song

Rong Huang

Weiting Chen

Adipose-mesenchymal stem cell-derived extracellular vesicles enhance angiogenesis and skin wound healing via bFGF-mediated VEGF expression

脂肪间充质干细胞衍生的细胞外囊泡通过bFGF介导的VEGF表达促进血管生成和皮肤伤口愈合

Abstract It is common practice that allograft tissues for knee multiligament reconstruction, meniscus transplantation, cartilage replacement and other advanced procedures are made available through commercial banks. The aim was to present the 10-year experience with a local, non-commercial tissue bank, established in 2014. The allograft bank was connected to an existing organ donor program. Following organ procurement, the musculoskeletal tissue is removed and stored immediately, fresh frozen to − 80 degrees Celsius (except hyaline cartilage, which is stored at 5 degrees Celsius). The donor is tested for contagious disease and the grafts for bacteria. When all results are negative, the grafts are released. When thawed before use a swap is cultured. Consecutive, prospectively collected data were analyzed. There were 31 donations, resulting in 1160 grafts. Only 40 grafts (3.4%) had a positive bacteria culture and were discarded. 552 recipients have been treated by use of these allografts. All grafts had negative bacterial cultures in swaps obtained before thawing, and there were no recorded transplantation related complications. The expenses for local grafts were 10–15% of the costs for grafts obtained from foreign banks. Through the local donation program, it was possible to establish a tissue bank with controlled quality grafts at minimal costs, minimizing the need for transportation of frozen grafts retrieved in other countries. Centers for specialized orthopedic surgery using allograft tissue can be self-providing and reduce costs by retrieving and handling allograft tissues locally.

Helia Azkia

Lene H. Harritshøj

Connie Nielsen

Niels Agerlin

Mette G. Jensen

Jens G. Hillingsø

Pia C. Andersen

Michael R. Krogsgaard

A local, non-commercial tissue bank connected to an organ donor program can produce musculoskeletal allografts of uniform quality at very low costs – ten years’ experience

一个与器官捐献计划相连的本地非商业性组织库能够以极低成本生产出质量一致的肌肉骨骼同种异体移植物——十年经验

Chander Gupt

Arundeep Kaur Lamba

Farrukh Faraz

Shruti Tandon

Jeyaseelan Augustine

Archita Datta

Sachin Dhingra

Correction to: Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane

更正：对本国制备的经冷冻干燥和化学处理的牛心包膜脱细胞的组织学评估

Abbreviation	Cell Tissue Bank
Journal Impact	1.52
Quartiles(Global)	ENGINEERING, BIOMEDICAL(Q4)

ISSN	1389-9333, 1573-6814
h-index	53

Publication Information	Publisher: Springer Netherlands，Publishing cycle: Quarterly，Journal Type: journal，Open Access Journals: No
Basic data	Year of publication: 2000，Proportion of original research papers: 89.02%，Self Citation Rate:7.10%， Gold OA Rate: 20.44%
Average review cycle	网友分享经验：较慢,6-12周
Average recruitment ratio	网友分享经验：容易

Cell and Tissue Banking

Journal Citation Format

A journal article with 1 author

A journal article with 2 authors

A journal article with 3 authors

A journal article with 5 or more authors

Books Citation Format

Thesis Citation Format

Web sites Citation Format

Patent Citation Format

Staying up late manually editing references? ivySCI automatically matches journals and helps you generate references with a single click.

Share Submission Experience

Mesenchymal stem cells for chronic wounds therapy.

Recent progress in mesenchymal stem cell-based therapy for acute lung injury

Curcumin-loaded human endometrial stem cells derived exosomes as an effective carrier to suppress alpha-synuclein aggregates in 6OHDA-induced Parkinson's disease mouse model.

Development of a sterilization process for amniotic membrane allograft tissue using supercritical carbon dioxide and NovaKill

Establishment of a dynamic osteosarcoma biobank: Ruijin experience.

Development of a sterilization process for amniotic membrane allograft tissue using supercritical carbon dioxide and NovaKill

Advances in preparation of acellular human dermis for tissue banking and transplantation

Adipose-mesenchymal stem cell-derived extracellular vesicles enhance angiogenesis and skin wound healing via bFGF-mediated VEGF expression

A local, non-commercial tissue bank connected to an organ donor program can produce musculoskeletal allografts of uniform quality at very low costs – ten years’ experience

Correction to: Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane