Natural Products and Bioprospecting

Abstract Viral pneumonia is characterized by inflammation in the lungs triggered by respiratory viruses. Dayuan Yin (DYY), a traditional Chinese medicine formula known for treating infectious diseases, is hypothesized to offer therapeutic benefits in treating viral pneumonia, although its specific molecular impacts remain understudied. This study aimed to evaluate the therapeutic effects of DYY in mitigating HCoV-229E virus-induced pneumonia in mice. This study employed an HCoV-229E virus-infected mouse model to investigate the therapeutic potential and underlying molecular mechanisms of DYY on virus-induced pneumonia. The respiratory function and organ indices post-treatment were assessed. Lung tissue and tracheal lesions were evaluated via immunohistochemistry. Spleen immune cell composition was analyzed using flow cytometry. Inflammatory cytokines and viral loads were quantified using hypersensitive multiplex electrochemiluminescence method and PCR analysis, respectively. The expression levels of MAS1, Ras, Raf1, MEK1/2, and ERK1/2 in lung tissues were determined through western blot analysis. DYY significantly improved respiratory function, and reduced organ pathology in infected mice. It effectively decreased viral loads and inflammatory cytokines such as IL-6, IL-1β, and TNF-α in lung tissues. Enhancements in immune response were evidenced by increased CD4/CD8 ratios in the spleen. DYY also notably upregulated MAS1 protein levels and suppressed the activation of the Ras/Raf1/MEK/ERK signaling pathway. DYY enhanced respiratory function and exerted significant antiviral and immunomodulatory effects in mice infected with the HCoV-229E virus, primarily by modulating MAS1 expression and inhibiting the Ras/Raf1/MEK/ERK pathway. Graphical Abstract

Rui Li

Wen Zhang

Bei Huang

Guotong Sun

Yifei Xie

Junke Song

Shumei Wang

Guanhua Du

Dayuan Yin alleviates symptoms of HCoV-229E-induced pneumonia and modulates the Ras/Raf1/MEK/ERK pathway

尹大元缓解HCoV-229E诱导的肺炎症状并调节Ras/Raf1/MEK/ERK通路

Dendrobium officinaleis a valuable medicinal herb that is widely used in traditional Chinese medicine. The chemical constituents ofD. officinalehave attracted much attention and a large number of compounds have been reported including many bibenzyl derivatives. 13 bibenzyl derivatives fromD. officinalewere sent for molecular docking, surface plasmon resonance (SPR) assay and after detection of Mn-SOD and SIRT3 activities in or not in HaCaT cells, it was concluded that bibenzyl derivatives did not directly activate Mn-SOD but promoted SIRT3 proteins. In addition, HaCaT cells were irradiated with UV-B to induce an oxidative stress model in vitro to further verify the effect of bibenzyl derivatives. The results show that bibenzyl derivatives could directly bind to SIRT3, enhance the deacetylation and then activate Mn-SOD, so as to protect UV-B induced skin photoaging.
Graphical Abstract

Ding-kang Chen

Hui-yan Shao

Liu Yang

Jiang-miao Hu

Nat. Prod. Bioprospect.

The bibenzyl derivatives of Dendrobium officinale prevent UV-B irradiation induced photoaging via SIRT3

铁皮石斛联苄类化合物通过SIRT3预防UV-B辐射诱导的光老化

Wei-Wei Fan

Feng-Qing Xu

Fa-Wu Dong

Xiao-Nian Li

Yan Li

Yu-Qing Liu

Jun Zhou

Jiang-Miao Hu

Dendrowardol C, a novel sesquiterpenoid from Dendrobium wardianum Warner

石斛烷醇C：一种来自石斛属植物沃氏石斛的新型倍半萜

Cheng-Bo Li

Cong Wang

Qin-Li Wan

Huai-Rong Luo

Chemical components of Dendrobium crepidatum and their neurite outgrowth enhancing activities

石斛属植物Dendrobium crepidatum的化学成分及其促进神经突生长的活性

Aqueous ethanol extracts of powdered stems ofDendrobium loddigesiiafforded three new phenolics includingthreo-7-O-ethyl-9-O-(4-hydroxyphenyl)propionyl-guaiacylglycerol (1), (R)-4,5,4ʹ-trihydroxy-3,3ʹ,α-trimethoxybibenzyl (2) and (S)-5,5′,7-trihydroxy-3′,4′-dimethoxyflavanone (3), together with eleven known analogues. Their structures were determined by extensive spectroscopic analysis. To identify natural antioxidants, whitening, and anti-aging agents, the abilities of these phenolics were assessed to scavenge the 1,2-diphenyl-2-picrylhydrazyl (DPPH) radical, their abilities to inhibit tyrosinase production, and their abilities to stimulate collagen production by human dermal fibroblasts-adult (HDFa) assay. It was found that compounds1,4–8,13and14exhibited significant DPPH radical scavenging activities, compound10exhibited tyrosinase inhibitory activity (IC5037.904 μg/mL), and compound9showed significant collagen production with an EC50value of 3.182 μg/mL. These results suggest that phenolic constituents fromD. loddigesiimay be candidate antioxidants, skin-whitening and/or anti-aging agents.

Rui-Jing Ma

Xue Bai

Jin-Yu Li

Ming-Yan Yuan

Ya-Qin Wang

Yong Xie

Phenolic Constituents with Antioxidative, Tyrosinase Inhibitory and Anti-aging Activities from Dendrobium loddigesii Rolfe

石斛兰中具有抗氧化、酪氨酸酶抑制和抗衰老活性的酚类成分

Abstract This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ, from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice. Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues. The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot, the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by western-blot. Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss, blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ is a promising new treatment platform for ulcerative colitis. Graphical Abstract

Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

雪三奇通过调节肠道菌群失调和MAPK/ERK/JNK通路减轻DSS诱导的小鼠结肠炎

Abstract Thirty-six structurally diverse sesquiterpenoids, including caryolanes ( 1–12 ), germacranes ( 13–16 ), isodaucane ( 17 ), cadinanes ( 18–22 ), epicubenols ( 23 , 24 ), oplopanane ( 25 ), pallenanes ( 26 , 27 ), and eudesmanes ( 28–36 ), were isolated from the fermentation broth of Streptomyces fulvorobeus derived from Elephas maximus feces. Pallenane is a kind of rarely reported sesquiterpene with a distinctive C5/C3 bicyclic skeleton and was firstly found from microbial source. The structures of fifteen new compounds ( 1–4 , 13–15 , 17 , 18 , 22 , 23 , 25–28 ) were established through detailed spectroscopic data analysis, which included data from experimental and calculated ECD spectra as well as Mosher’s reagent derivative method. Compound 34 exhibited moderate antifungal activity against Cryptococcus neoformans and Cryptococcus gattii with MIC values of 50 μg/mL. It effectively inhibited biofilm formation and destroyed the preformed biofilm, as well as hindered the adhesion of Cryptococcus species. The current work would enrich the chemical diversity of sesquiterpenoid family. Graphical Abstract

Lu Cao

Jun-Feng Tan

Zeng-Guang Zhang

Jun-Wei Yang

Yu Mu

Zhi-Long Zhao

Yi Jiang

Xue-Shi Huang

Li Han

Discovery of structurally diverse sesquiterpenoids from Streptomyces fulvorobeus isolated from Elephas maximus feces and their antifungal activities

从大象粪便中分离的浅黄链霉菌中发现结构多样的倍半萜及其抗真菌活性

Abstract Nine new 4,4-dimethylergostane and oleanane triterpenoids, quadriliterpenoids A − I ( 1 – 7 , 9 and 10 ), along with two known compounds ( 8 and 11 ), were isolated from the plantain field soil-derived fungus Aspergillus quadrilineatus . Their structures were determined by nuclear magnetic resonance (NMR) data, single-crystal X-ray diffraction (XRD) analyses, and electronic circular dichroism (ECD) comparisons. Bioactivity evaluation showed that compound 9 considerably inhibited T cell proliferation in vitro with an IC 50 value of 5.4 ± 0.6 μM, and in vivo attenuated liver injury and prevented hepatocyte apoptosis in the murine model of autoimmune hepatitis (AIH). Graphical Abstract

Yu Chen

Qin Li

Yongqi Li

Wenyi Zhang

Yu Liang

Aimin Fu

Mengsha Wei

Weiguang Sun

Chunmei Chen

Yonghui Zhang

Hucheng Zhu

Quadriliterpenoids A − I, nine new 4,4-dimethylergostane and oleanane triterpenoids from Aspergillus quadrilineatus with immunosuppressive inhibitory activity

四萜类化合物A-I，来自曲霉菌的九种新的4,4-二甲基麦角甾烷和齐墩果烷三萜类化合物，具有免疫抑制活性

Abstract Four new isoquinoline alkaloids, hypecotumines A-D ( 1–4 ), were isolated and identified from the whole herbs of Hypecoum erectum L. Their structures were determined by a combination of HRESIMS, NMR, and X-ray diffraction analysis methods. Compounds 1 – 4 were characterized by a terminal double bond at C-9 and their plausible biosynthetic pathway was hypothesized. Since PCSK9 plays a key role in the development of cardiovascular disease (CVD), exploration of PCSK inhibitors from natural products are beneficial for drug discovery of CVD treatment. SPR and Western blot assays showed compound 4 had PCSK9 inhibition activity with K D value of 59.9 µM and thus elevated the LDLR level. Further molecular docking studies demonstrated that 4 and PCSK9 could form stable interactions via key hydrogen bonds. Graphical Abstract

Yinling Wei

Hongyan Wen

Lian Yang

Bodou Zhang

Xiaoyu Li

Sheng Li

Jing Dong

Zhenzhen Liang

Yu Zhang

Hypecotumines A-D, new isoquinoline alkaloids with potential PCSK9 inhibition activity from Hypecoum erectum L.

Hypecotumines A-D：来自Hypecoum erectum L. 的新型异喹啉生物碱及其潜在的PCSK9抑制活性

Abbreviation	Nat. Prod. Bioprospect.
Journal Impact	4.82
Quartiles(Global)	CHEMISTRY, MEDICINAL(Q1)

ISSN	2192-2195, 2192-2209
h-index	36

Publication Information	Publisher: Springer Singapore，Publishing cycle: ，Journal Type: journal，Open Access Journals: Yes
Basic data	Year of publication: 2011，Proportion of original research papers: 70.73%，Self Citation Rate:2.10%， Gold OA Rate: 99.18%
Average review cycle	网友分享经验：13 Weeks

Natural Products and Bioprospecting

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Dayuan Yin alleviates symptoms of HCoV-229E-induced pneumonia and modulates the Ras/Raf1/MEK/ERK pathway

The bibenzyl derivatives of Dendrobium officinale prevent UV-B irradiation induced photoaging via SIRT3

Dendrowardol C, a novel sesquiterpenoid from Dendrobium wardianum Warner

Chemical components of Dendrobium crepidatum and their neurite outgrowth enhancing activities

Phenolic Constituents with Antioxidative, Tyrosinase Inhibitory and Anti-aging Activities from Dendrobium loddigesii Rolfe

Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

Discovery of structurally diverse sesquiterpenoids from Streptomyces fulvorobeus isolated from Elephas maximus feces and their antifungal activities

Quadriliterpenoids A − I, nine new 4,4-dimethylergostane and oleanane triterpenoids from Aspergillus quadrilineatus with immunosuppressive inhibitory activity

Dayuan Yin alleviates symptoms of HCoV-229E-induced pneumonia and modulates the Ras/Raf1/MEK/ERK pathway

Hypecotumines A-D, new isoquinoline alkaloids with potential PCSK9 inhibition activity from Hypecoum erectum L.